##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088505_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	936212
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.779032954074506	31.0	31.0	31.0	28.0	34.0
2	30.898388399208727	31.0	31.0	33.0	28.0	34.0
3	30.85390381665691	31.0	31.0	34.0	27.0	34.0
4	34.64945760148343	35.0	35.0	37.0	32.0	37.0
5	34.43415914344187	35.0	35.0	37.0	30.0	37.0
6	34.71853704075573	35.0	35.0	37.0	32.0	37.0
7	34.56430915220057	35.0	35.0	37.0	30.0	37.0
8	34.72620303948251	35.0	35.0	37.0	32.0	37.0
9	36.14074162689647	38.0	35.0	39.0	32.0	39.0
10	35.84290203500917	37.0	35.0	39.0	30.0	39.0
11	36.05848568486625	37.0	35.0	39.0	31.0	39.0
12	35.76983845539258	37.0	35.0	39.0	30.0	39.0
13	35.94357581402503	37.0	35.0	39.0	30.0	39.0
14	36.61296373043712	38.0	36.0	40.0	31.0	41.0
15	36.70255882214712	38.0	36.0	40.0	31.0	41.0
16	36.47638889482297	38.0	35.0	40.0	30.0	41.0
17	36.60293074645486	38.0	36.0	40.0	31.0	41.0
18	36.59783361033612	38.0	36.0	40.0	30.0	41.0
19	36.68773525654446	38.0	35.0	40.0	30.0	41.0
20	36.590672839057824	38.0	35.0	40.0	30.0	41.0
21	36.44294775115038	38.0	35.0	40.0	30.0	41.0
22	36.28159861228013	38.0	35.0	40.0	30.0	41.0
23	36.09216181804976	38.0	34.0	40.0	29.0	41.0
24	36.19491098170072	38.0	35.0	40.0	30.0	41.0
25	36.1011960966106	38.0	34.0	40.0	29.0	41.0
26	35.776370095662095	38.0	34.0	40.0	28.0	41.0
27	35.68633493268619	38.0	34.0	40.0	27.0	41.0
28	35.49685648122434	38.0	34.0	40.0	27.0	41.0
29	35.2725226764878	38.0	34.0	40.0	27.0	41.0
30	35.09261684319364	37.0	33.0	40.0	27.0	41.0
31	34.75703900398628	37.0	33.0	40.0	25.0	41.0
32	34.55768244799255	37.0	33.0	40.0	25.0	41.0
33	34.32219411842617	36.0	33.0	39.0	25.0	40.0
34	34.05454106548517	36.0	33.0	39.0	24.0	40.0
35	33.7892144087023	36.0	33.0	39.0	23.0	40.0
36	33.692304734397766	36.0	32.0	39.0	22.0	40.0
37	33.48200407599988	36.0	32.0	39.0	21.0	40.0
38	33.27612869734633	36.0	31.0	39.0	20.0	40.0
39	33.037251178151955	36.0	31.0	39.0	18.0	40.0
40	32.7997803916207	36.0	31.0	39.0	18.0	40.0
41	32.58373103527833	36.0	31.0	39.0	15.0	40.0
42	32.22506227222039	35.0	30.0	39.0	15.0	40.0
43	31.78126535442827	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	2.0
14	16.0
15	25.0
16	49.0
17	101.0
18	228.0
19	474.0
20	989.0
21	1707.0
22	2905.0
23	4735.0
24	7176.0
25	10147.0
26	13886.0
27	19144.0
28	24308.0
29	30331.0
30	37382.0
31	44559.0
32	53656.0
33	64248.0
34	77392.0
35	94639.0
36	121922.0
37	145047.0
38	130078.0
39	51064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14711625144732	25.736264863086568	16.317778451889104	18.79884043357701
2	23.629049830593924	26.803010429261747	30.305422276151127	19.2625174639932
3	24.153076439951633	25.06782651792543	32.479822946084866	18.299274096038076
4	18.516532580227555	19.71412457862108	31.806257557049044	29.96308528410232
5	11.586585089701904	42.32962192323961	33.197715901953835	12.886077085104656
6	31.763852631668893	38.83073491901407	12.57994984042076	16.825462608896274
7	23.532917757943714	36.15260218839323	21.03391112269443	19.280568930968627
8	28.089043934493468	36.036389193900526	18.171204812585184	17.70336205902082
9	24.43185945063725	14.298791299406544	22.142954800835707	39.1263944491205
10	16.98749855801891	30.662499519339637	31.48079708442105	20.869204838220405
11	33.750582133106604	23.108548063900056	22.44181873336381	20.69905106962953
12	19.9080977385464	30.34216609058632	31.070313134204646	18.679423036662637
13	33.30794734525941	21.61615104271255	26.37212511696069	18.703776495067356
14	19.486077939611967	24.349292681572123	29.78545457652754	26.379174802288368
15	22.498750283055546	31.595407877702915	26.240744617672068	19.665097221569475
16	19.995257484415923	29.010950511209	28.58583312326695	22.407958881108126
17	20.82509089821536	28.515122643162016	28.712407018923063	21.947379439699553
18	21.720294121416945	25.86347963922701	31.185992061627065	21.230234177728978
19	22.07790543167573	25.398093594185934	33.53567354402635	18.988327430111983
20	22.409561082318962	25.091966349502037	33.15563141681585	19.342841151363153
21	21.230981871627367	25.5441075312002	34.08405361178878	19.140856985383653
22	19.77426053073449	26.14824420109975	33.70892490162485	20.36857036654091
23	20.81601175802062	26.227820194571315	34.179117550298436	18.77705049710963
24	20.536481053436614	26.39188559856101	32.553844642025524	20.51778870597685
25	20.624495306618588	26.68626336769877	33.11354693167787	19.575694394004778
26	19.809936211029125	28.000388800827164	32.97073739708527	19.218937591058435
27	19.318060439302208	27.35363357871935	32.85879693915481	20.469509042823635
28	19.59492080853482	27.93598031215152	32.924594002213176	19.544504877100486
29	19.766569964922475	26.77769565013053	32.084399687250325	21.371334697696675
30	20.144582637265916	27.339854648306154	33.688096285883965	18.827466428543964
31	20.844317312745403	27.3010813790039	32.92673027049429	18.927871037756404
32	20.17577215417021	27.299479177793064	32.51998478976984	20.00476387826689
33	19.886735055735237	27.280252763263025	32.91476716812004	19.918245012881698
34	19.087770718597923	27.353954018961517	33.76553601107442	19.792739251366143
35	18.671625657436564	28.886940137490225	33.02414410411318	19.417290100960038
36	21.171059546342068	26.895617659248117	32.12808637359914	19.805236420810672
37	20.082310416871394	28.083382823548515	32.12509559800558	19.709211161574515
38	20.125676662978044	26.139592314561234	33.29331390753376	20.44141711492696
39	19.180057508342127	26.296608033223244	34.08373317154661	20.439601286888013
40	18.456396628114145	26.315193567268953	33.56558129996197	21.66282850465493
41	19.431282658201347	25.630412769757278	33.73958035145886	21.19872422058252
42	19.079866525957794	27.08841587161882	32.63598415743443	21.195733444988957
43	19.195865893622386	26.894015458037284	32.116764151709226	21.793354496631103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	708.0
1	505.0
2	302.0
3	452.5
4	603.0
5	603.0
6	1219.0
7	1835.0
8	2071.5
9	2308.0
10	3744.5
11	5181.0
12	5181.0
13	10248.5
14	15316.0
15	27317.0
16	39318.0
17	34407.5
18	29497.0
19	29497.0
20	32636.5
21	35776.0
22	27781.0
23	19786.0
24	20889.5
25	21993.0
26	21993.0
27	22951.0
28	23909.0
29	24940.5
30	25972.0
31	28022.0
32	30072.0
33	30072.0
34	32879.0
35	35686.0
36	38014.5
37	40343.0
38	42127.5
39	43912.0
40	43912.0
41	45620.0
42	47328.0
43	49623.5
44	51919.0
45	55641.5
46	59364.0
47	59364.0
48	77769.5
49	96175.0
50	87677.0
51	79179.0
52	69743.0
53	60307.0
54	60307.0
55	53085.0
56	45863.0
57	40009.5
58	34156.0
59	30142.5
60	26129.0
61	26129.0
62	22784.5
63	19440.0
64	16687.5
65	13935.0
66	11968.0
67	10001.0
68	10001.0
69	8506.5
70	7012.0
71	6086.5
72	5161.0
73	4253.5
74	3346.0
75	3346.0
76	2641.5
77	1937.0
78	1527.0
79	1117.0
80	871.5
81	626.0
82	626.0
83	480.5
84	335.0
85	263.0
86	191.0
87	141.5
88	92.0
89	92.0
90	70.5
91	49.0
92	36.5
93	24.0
94	14.5
95	5.0
96	5.0
97	3.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	936212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.91263322227575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.44777604203247	46.37249752233014
2	8.698294229672552	9.552924813868929
3	2.5102835670035626	4.135388423963182
4	1.166667333320043	2.562591014680562
5	0.6705726509694121	1.8411455015786231
6	0.4408142235342986	1.452376186566073
7	0.33396129333659463	1.2837085808000483
8	0.24743311252345557	1.086976300403728
9	0.19405832105331675	0.9590628066958272
>10	1.1527651220499178	11.771683607478343
>50	0.08371382374952788	3.139719630962665
>100	0.04429412268836273	4.54402076670518
>500	0.004878207326310857	1.8979494357092377
>1k	0.0037074375679962512	3.506700795035072
>5k	1.9512829305243427E-4	0.6719381759912285
>10k+	5.853848791573028E-4	5.221316437231155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20242	2.162117127317317	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17532	1.872652775226124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10955	1.170140950981188	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6271	0.669826919543864	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3481	0.3718174943282077	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3256	0.34778447616565483	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2216	0.23669852554763235	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2123	0.22676487804044382	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2088	0.22302640854849118	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2047	0.21864705857220376	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1959	0.20924747813529415	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1616	0.1726104771141579	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1559	0.16652211251297783	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1469	0.15690890524795664	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1459	0.15584077110739877	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1453	0.155199890623064	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1325	0.1415277736239228	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1297	0.13853699803036065	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1139	0.1216604786095457	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1108	0.11834926277381617	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1079	0.11525167376619826	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1043	0.11140639086018979	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1010	0.10788154819634868	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	964	0.10296813114978232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.0681341405579079E-4	0.0
9	0.0	0.0	0.0	1.0681341405579079E-4	0.0
10	0.0	0.0	0.0	1.0681341405579079E-4	0.0
11	0.0	0.0	0.0	2.1362682811158158E-4	0.0
12	0.0	0.0	0.0	2.1362682811158158E-4	0.0
13	0.0	0.0	0.0	3.204402421673723E-4	0.0
14	0.0	0.0	0.0	4.2725365622316315E-4	0.0
15	0.0	0.0	0.0	5.340670702789539E-4	0.0
16	0.0	0.0	0.0	9.61320726502117E-4	0.0
17	0.0	0.0	0.0	0.0013885743827252801	0.0
18	0.0	0.0	0.0	0.0016022012108368618	0.0
19	0.0	0.0	0.0	0.0022430816951716065	0.0
20	0.0	0.0	0.0	0.004272536562231631	0.0
21	0.0	0.0	0.0	0.0077973792260727275	0.0
22	0.0	0.0	0.0	0.012817609686694894	0.0
23	0.0	0.0	0.0	0.015274318209978082	0.0
24	0.0	0.0	0.0	0.021149055983046575	0.0
25	0.0	0.0	0.0	0.02563521937338979	0.0
26	0.0	0.0	0.0	0.03172358397456986	0.0
27	0.0	0.0	0.0	0.07701247153422515	0.0
28	0.0	0.0	0.0	0.19205051847231183	0.0
29	0.0	0.0	0.0	0.35430009442305804	0.0
30	0.0	0.0	0.0	0.5715585786125364	0.0
31	0.0	0.0	0.0	0.8783267037807676	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	25	0.0054958295	29.599998	14
GGTATCA	4675	0.0	26.394651	1
GCCGATA	50	2.7015837E-4	22.199999	2
CGTTTAT	100	5.347829E-10	22.199999	1
CCGTATA	65	6.90038E-5	19.923079	2
TATACCG	65	6.90038E-5	19.923079	5
CGAGTTC	75	9.2627615E-6	19.733334	14
CGTATAC	75	9.2627615E-6	19.733334	3
CGTTCGC	130	6.9667294E-10	18.500002	23
AGCCGTC	90	2.1513479E-6	18.5	12
TATTACC	90	2.1513479E-6	18.5	2
CTCGTTT	50	0.007033932	18.499998	9
TTCGTTA	100	2.873512E-7	18.499998	30
CGTGTCT	100	2.873512E-7	18.499998	35
GTATCAA	6975	0.0	17.744087	2
GTATACT	75	2.0667506E-4	17.266666	4
TGTATAC	65	0.0015796375	17.076925	3
CCGAGTT	65	0.0015796375	17.076925	13
GATAGAC	65	0.0015796375	17.076925	3
ACGTTCG	110	7.803792E-7	16.818182	22
>>END_MODULE