FastQCFastQC Report
Wed 25 May 2016
SRR2088504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336675
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT181931.3610638337666225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128190.9590214524847102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117510.8791217012362765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52300.3912693811135841No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG28460.21291637832681842No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA23120.1729665027026016No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA23000.17206875268857424No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG21420.1602483775038809No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA20040.14992425234256643No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG17820.13331587708306059No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17600.13167000205734378No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16450.12306656442291507No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC14180.10608412665756449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34400.024.899711
GACCGTC400.001931350723.12500222
TAGTTCG603.7268706E-521.5833349
GTATTGG3000.017.8833331
GTTCTAT752.0675843E-417.2666661
CGTATAC752.0675843E-417.2666663
TATACCG650.00158011617.0769235
CAATGCG803.3830805E-416.187519
CGAACAC1059.3452945E-615.85714232
TAGTACT1954.1836756E-1115.1794864
GTATCAA57250.015.0585162
CCGTCGG750.00410487214.824
TAGGAAC2750.014.79999930
GTCTAGA908.275258E-414.3888881
ATGCGAC908.275258E-414.38888821
GTACTAG1458.9123023E-714.0344831
ATCGTCA1203.3011485E-513.87500110
CGTTTAT1203.3011485E-513.8750011
TATACTG2151.9645086E-1013.7674425
ATCAACG92200.013.7646432
Produced by FastQC (version 0.11.5)