FastQCFastQC Report
Wed 25 May 2016
SRR2088503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930717
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT514831.756669101793179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT372421.270747056095829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT362721.2376493533834894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146760.500764829903399No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG93280.3182838875264995No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83220.283957816466073No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA76600.2613694873984762No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG74510.25423812671097207No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA66870.2281694206571293No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64210.2190931434184877No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA57480.19612947957786442No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC48870.16675100325278763No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46100.1572993912411195No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT40670.13877150199080976No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39810.1358370664926023No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT35420.12085779691454344No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33220.11335110145401278No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.10731162374258585No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA31160.10632210479551592No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA31120.10618561942350627No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG31060.1059808913654918No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG30050.10253463572224818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109150.023.8983061
GTATTGG7750.019.0967751
ATTGGAC7100.017.718313
TATACCG1603.45608E-1117.343755
ACGTTTA1107.817762E-716.81818226
CTAGTAC3600.016.4444453
TTAACGG1504.6802597E-916.03333335
TTGGACC12100.015.5950414
GGACCCT12000.015.4166676
GACCCTC11500.014.9608697
TGATACT14100.014.826241535
CGTATAC1252.9623516E-614.7999993
TTTTTAC19200.014.5494791
TGGACCC12900.014.4844965
TTATACG908.279408E-414.3888894
GTATCAA181400.014.3798242
GTATTAG4650.014.322581
GATACTG14550.014.24055136
CCGTATA1304.449337E-614.2307692
CGGTATA1956.7484507E-1014.23076826
Produced by FastQC (version 0.11.5)