##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088503_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2930717
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.648836445142948	31.0	31.0	34.0	30.0	34.0
2	31.801466671807617	31.0	31.0	34.0	30.0	34.0
3	31.92187952640941	31.0	31.0	34.0	30.0	34.0
4	35.60304219069941	37.0	35.0	37.0	33.0	37.0
5	35.51853249563162	37.0	35.0	37.0	33.0	37.0
6	35.56972065197697	37.0	35.0	37.0	33.0	37.0
7	35.42258737366999	37.0	35.0	37.0	33.0	37.0
8	35.46669910469008	37.0	35.0	37.0	33.0	37.0
9	37.0444846772991	39.0	37.0	39.0	33.0	39.0
10	36.894647282559184	39.0	37.0	39.0	33.0	39.0
11	36.96424765680207	39.0	37.0	39.0	33.0	39.0
12	36.805815778186705	39.0	37.0	39.0	32.0	39.0
13	36.87374386540905	39.0	37.0	39.0	33.0	39.0
14	37.86501494344217	40.0	37.0	41.0	33.0	41.0
15	37.87354971496736	40.0	37.0	41.0	33.0	41.0
16	37.762713697706054	40.0	37.0	41.0	32.0	41.0
17	37.814889666931336	40.0	37.0	41.0	32.0	41.0
18	37.783213800581905	40.0	37.0	41.0	32.0	41.0
19	37.85529411403421	40.0	37.0	41.0	32.0	41.0
20	37.75837585137016	40.0	37.0	41.0	32.0	41.0
21	37.62862739732291	40.0	37.0	41.0	32.0	41.0
22	37.508518222673835	39.0	36.0	41.0	32.0	41.0
23	37.346950933849975	39.0	36.0	41.0	31.0	41.0
24	37.496403098627404	39.0	36.0	41.0	32.0	41.0
25	37.44152028326174	39.0	36.0	41.0	32.0	41.0
26	37.23979387979119	39.0	36.0	41.0	31.0	41.0
27	37.192202795425146	39.0	36.0	41.0	31.0	41.0
28	37.04810290451108	39.0	36.0	41.0	31.0	41.0
29	36.83052952570992	39.0	35.0	41.0	30.0	41.0
30	36.6938660402898	39.0	35.0	40.0	30.0	41.0
31	36.42573745605598	38.0	35.0	40.0	30.0	41.0
32	36.269099336442245	38.0	35.0	40.0	30.0	41.0
33	36.08611271576205	38.0	35.0	40.0	30.0	41.0
34	35.90540983656901	38.0	35.0	40.0	29.0	41.0
35	35.69703284213385	38.0	35.0	40.0	28.0	41.0
36	35.61687600679288	38.0	35.0	40.0	27.0	41.0
37	35.46773264016962	38.0	35.0	40.0	26.0	41.0
38	35.323430409691554	38.0	34.0	40.0	25.0	41.0
39	35.18125530373625	38.0	34.0	40.0	25.0	41.0
40	34.98112407305106	38.0	34.0	40.0	24.0	41.0
41	34.83211275602523	38.0	34.0	40.0	24.0	41.0
42	34.53827339862566	38.0	33.0	40.0	22.0	41.0
43	34.14102897004385	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	6.0
15	20.0
16	47.0
17	94.0
18	218.0
19	446.0
20	1091.0
21	2202.0
22	3992.0
23	6772.0
24	11089.0
25	16712.0
26	24768.0
27	34934.0
28	47216.0
29	62235.0
30	78055.0
31	96149.0
32	116821.0
33	143176.0
34	176981.0
35	219714.0
36	288747.0
37	420974.0
38	555131.0
39	623124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.9732157693834	21.51152772512665	15.010422364220085	22.504834141269868
2	18.539865841703584	24.23512744492218	35.33466383823481	21.890342875139428
3	19.111807793110014	24.542833716117933	33.94206946627737	22.40328902449469
4	13.357243295753223	16.611566384608274	36.23127036830919	33.799919951329315
5	12.048860398325733	39.160212330293234	34.89347487321362	13.897452398167411
6	31.725956480956707	38.316255032471574	13.580158029587983	16.377630456983734
7	25.111772989340153	34.466480386881436	21.14103818280646	19.280708440971953
8	27.46112981908523	34.3485911468081	18.96607553714671	19.22420349695996
9	24.572178071100005	14.505938307929425	21.974656713698387	38.94722690727218
10	16.147516119775467	28.77128702634884	32.950571481313276	22.130625372562417
11	33.247802500207285	22.960695283782094	23.344867484646247	20.446634731364373
12	21.503099753405053	28.117692701137642	30.70129255059427	19.677914994863034
13	32.15315569534691	21.473209456934942	25.94030744012472	20.43332740759343
14	20.403573596495328	23.259222913710197	28.864438292745426	27.47276519704905
15	22.223264818813963	30.72104880819267	25.44994961983706	21.60573675315631
16	21.196041787726347	28.691954903868233	26.77508609667873	23.33691721172669
17	21.384869299901695	27.51040103838071	27.39449083620152	23.71023882551608
18	22.6029330024018	25.698762453010644	30.426956952854884	21.271347591732674
19	22.33651355623897	25.06410547316578	31.94539083780522	20.65399013279003
20	23.160305140346203	24.868658420447964	31.233005438600863	20.73803100060497
21	22.150415751503814	25.458855290360688	31.949826612395533	20.440902345739968
22	20.662929924656662	26.08658563757606	31.52655135245061	21.723933085316666
23	21.92303112173574	26.088223462040176	31.863192522512406	20.125552893711678
24	21.337713603872363	26.115520536442105	30.353869036143717	22.192896823541815
25	21.373779863425913	26.346624392597445	31.39354635742721	20.886049386549434
26	20.85626145410833	27.370878866843846	31.220141692288948	20.55271798675887
27	19.719918368099002	27.327305911829768	31.042096524502366	21.910679195568868
28	20.61266918641411	27.044883555798805	30.752372201068884	21.590075056718202
29	20.60888171734084	26.908671154533177	30.09151002979817	22.390937098327814
30	20.802145004106503	27.342319302750827	31.758508242181012	20.097027450961658
31	21.80974826296773	27.09401828972228	31.25808462570763	19.83814882160236
32	20.947160711866754	27.037786316454298	30.513966377511032	21.501086594167912
33	20.604650670808542	26.827087023414407	31.187043989576612	21.38121831620044
34	19.92601127983357	27.258892619109933	31.90157903339012	20.913517067666376
35	19.349531189807816	29.353055924539966	30.856237569168226	20.441175316483985
36	21.67124973172094	27.32467856841858	30.41661818592515	20.587453513935326
37	20.724348342061006	28.311877264164366	30.33227022602319	20.63150416775144
38	20.36552829904764	26.84343114671256	31.564528407212293	21.226512147027503
39	20.035028970726277	26.480925998654936	32.359009757680454	21.125035272938327
40	18.91444994518406	26.673882193333576	32.30223184292444	22.109436018557915
41	19.990705346166145	25.920141726410296	32.525453668846225	21.563699258577337
42	19.25122760061787	27.502962585606184	31.70473300560921	21.54107680816674
43	19.061205841437438	27.49726432132478	31.20942759058619	22.232102246651586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1529.0
1	1276.5
2	1024.0
3	2035.5
4	3047.0
5	3047.0
6	4571.5
7	6096.0
8	6563.5
9	7031.0
10	10612.0
11	14193.0
12	14193.0
13	25898.0
14	37603.0
15	65708.0
16	93813.0
17	86257.0
18	78701.0
19	78701.0
20	80025.5
21	81350.0
22	65007.5
23	48665.0
24	53731.5
25	58798.0
26	58798.0
27	65172.0
28	71546.0
29	75912.0
30	80278.0
31	86805.0
32	93332.0
33	93332.0
34	102509.5
35	111687.0
36	121604.0
37	131521.0
38	137974.0
39	144427.0
40	144427.0
41	148527.0
42	152627.0
43	159499.0
44	166371.0
45	177113.5
46	187856.0
47	187856.0
48	243379.0
49	298902.0
50	276477.5
51	254053.0
52	228536.0
53	203019.0
54	203019.0
55	181590.5
56	160162.0
57	139560.0
58	118958.0
59	106198.5
60	93439.0
61	93439.0
62	82389.0
63	71339.0
64	61825.5
65	52312.0
66	44583.5
67	36855.0
68	36855.0
69	31497.0
70	26139.0
71	22038.0
72	17937.0
73	14555.5
74	11174.0
75	11174.0
76	8887.5
77	6601.0
78	5212.0
79	3823.0
80	3047.0
81	2271.0
82	2271.0
83	1713.5
84	1156.0
85	852.0
86	548.0
87	416.0
88	284.0
89	284.0
90	222.0
91	160.0
92	105.0
93	50.0
94	40.0
95	30.0
96	30.0
97	18.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2930717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.46644309670268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36898023999179	36.24963854914781
2	12.745522512172238	12.099692381235332
3	4.5409111810050415	6.466227065410704
4	2.06213702720002	3.915292394367735
5	1.1157314121836728	2.647990079381002
6	0.6723571861203502	1.914864246938444
7	0.4140279771065961	1.3756704791041219
8	0.3255449187862977	1.2361967490392398
9	0.23855436713434924	1.019099456374726
>10	1.3383487734020079	11.791080440089676
>50	0.10809892046629681	3.554081429769798
>100	0.05998829468438155	5.3360558525312
>500	0.004466365914115397	1.4913350303446953
>1k	0.004538404074002323	4.361814733921109
>5k	5.042671193335914E-4	1.7649892296297918
>10k+	2.881526396191951E-4	4.7759718827146465
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	51483	1.756669101793179	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37242	1.270747056095829	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	36272	1.2376493533834894	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14676	0.500764829903399	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9328	0.3182838875264995	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8322	0.283957816466073	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7660	0.2613694873984762	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7451	0.25423812671097207	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6687	0.2281694206571293	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6421	0.2190931434184877	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5748	0.19612947957786442	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4887	0.16675100325278763	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4610	0.1572993912411195	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4067	0.13877150199080976	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3981	0.1358370664926023	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3542	0.12085779691454344	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3322	0.11335110145401278	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3145	0.10731162374258585	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3116	0.10632210479551592	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3112	0.10618561942350627	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3106	0.1059808913654918	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3005	0.10253463572224818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	3.412134300241204E-5	0.0
12	0.0	0.0	0.0	1.3648537200964815E-4	0.0
13	0.0	0.0	0.0	1.7060671501206018E-4	0.0
14	0.0	0.0	0.0	3.753347730265324E-4	0.0
15	0.0	0.0	0.0	6.483055170458287E-4	0.0
16	0.0	0.0	0.0	7.847908890554769E-4	0.0
17	0.0	0.0	0.0	8.87154918062713E-4	0.0
18	0.0	0.0	0.0	0.0011601256620820094	0.0
19	0.0	0.0	0.0	0.0016719458071181898	0.0
20	0.0	0.0	0.0	0.0029685568412098472	0.0
21	0.0	0.0	0.0	0.005425293537383514	0.0
22	0.0	0.0	0.0	0.008462093064598185	0.0
23	0.0	0.0	0.0	0.010338766929730847	0.0
24	0.0	0.0	0.0	0.012863746311909337	0.0
25	0.0	0.0	0.0	0.01508163360706612	0.0
26	0.0	0.0	0.0	0.01992686431340863	0.0
27	0.0	0.0	0.0	0.060019442341242776	0.0
28	0.0	0.0	0.0	0.15869836630421838	0.0
29	0.0	0.0	0.0	0.3144622971102293	0.0
30	0.0	0.0	0.0	0.5272771134162733	0.0
31	0.0	0.0	0.0	0.7852003451715058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10915	0.0	23.898306	1
GTATTGG	775	0.0	19.096775	1
ATTGGAC	710	0.0	17.71831	3
TATACCG	160	3.45608E-11	17.34375	5
ACGTTTA	110	7.817762E-7	16.818182	26
CTAGTAC	360	0.0	16.444445	3
TTAACGG	150	4.6802597E-9	16.033333	35
TTGGACC	1210	0.0	15.595041	4
GGACCCT	1200	0.0	15.416667	6
GACCCTC	1150	0.0	14.960869	7
TGATACT	1410	0.0	14.8262415	35
CGTATAC	125	2.9623516E-6	14.799999	3
TTTTTAC	1920	0.0	14.549479	1
TGGACCC	1290	0.0	14.484496	5
TTATACG	90	8.279408E-4	14.388889	4
GTATCAA	18140	0.0	14.379824	2
GTATTAG	465	0.0	14.32258	1
GATACTG	1455	0.0	14.240551	36
CCGTATA	130	4.449337E-6	14.230769	2
CGGTATA	195	6.7484507E-10	14.230768	26
>>END_MODULE