FastQCFastQC Report
Wed 25 May 2016
SRR2088502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227910
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT318142.5909064996620277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT251822.050801768859281No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT175261.4273033039880774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91110.741992491306366No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG53980.4396087661147804No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA48880.398074777467404No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG33760.2749387170069467No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT31890.2597095878362421No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA31730.25840656074142243No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30900.25164710768704546No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG29240.23812820157829157No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA24260.19757148325202986No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA24190.19700140889804627No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC22410.18250523246817765No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT22310.18169084053391538No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG21520.17525714425324332No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.1628783868524566No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT17780.14479888591183393No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.14260002768932575No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14880.12118151981822772No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG14690.11963417514312938No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14120.11499214111783437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC13940.11352623563616226No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13500.10994291112540822No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13360.10880276241744102No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC12400.10098459984852311No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12350.10057740388139196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67200.026.4561021
TAGACTG1054.0017767E-1122.9047645
ACGGTGT951.6763443E-719.47368429
TCGAACG500.007035149718.526
GATAGAC953.6067922E-617.5263163
TATGCGG852.72336E-517.4117645
GTATCAA102700.017.31112
GACCCTC3450.017.159427
ATAGACA1655.4569682E-1116.8181824
CGGTGTG1107.808667E-716.81818230
GGACCCT3650.016.7260276
ACCGTGA1352.2195309E-816.4444458
GAACTAG803.382801E-416.18756
CTAGGAC803.382801E-416.18753
TACCGTG1151.2422879E-616.0869567
CTAGACT1059.344056E-615.8571444
GTATTGG1902.7284841E-1115.5789481
TTATGCG957.0603004E-515.5789484
GCCGATA957.0603004E-515.5789482
TAGGAAC2150.015.48837230
Produced by FastQC (version 0.11.5)