##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1227910
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.852042087775164	31.0	31.0	31.0	28.0	34.0
2	30.981394401869842	31.0	31.0	33.0	28.0	34.0
3	30.942414346328313	31.0	31.0	34.0	27.0	34.0
4	34.743524362534714	35.0	35.0	37.0	32.0	37.0
5	34.57482551652809	35.0	35.0	37.0	32.0	37.0
6	34.814037673770876	35.0	35.0	37.0	32.0	37.0
7	34.646926077644125	35.0	35.0	37.0	31.0	37.0
8	34.81127036997825	35.0	35.0	37.0	32.0	37.0
9	36.227453966495915	38.0	35.0	39.0	32.0	39.0
10	35.95303238836723	37.0	35.0	39.0	30.0	39.0
11	36.15157136923716	38.0	35.0	39.0	32.0	39.0
12	35.874203321090306	37.0	35.0	39.0	30.0	39.0
13	36.03740013518906	37.0	35.0	39.0	31.0	39.0
14	36.696059157430106	38.0	36.0	40.0	31.0	41.0
15	36.792444071633916	38.0	36.0	40.0	31.0	41.0
16	36.57844304550008	38.0	35.0	40.0	30.0	41.0
17	36.73634549763419	38.0	36.0	40.0	31.0	41.0
18	36.73170264921696	38.0	36.0	40.0	31.0	41.0
19	36.841517700808694	39.0	36.0	40.0	31.0	41.0
20	36.74828529778241	39.0	36.0	40.0	30.0	41.0
21	36.60881009194485	38.0	35.0	40.0	30.0	41.0
22	36.46182619247339	38.0	35.0	40.0	30.0	41.0
23	36.26465294687721	38.0	35.0	40.0	30.0	41.0
24	36.357601941510374	38.0	35.0	40.0	30.0	41.0
25	36.26080820255556	38.0	35.0	40.0	30.0	41.0
26	35.95370996245653	38.0	34.0	40.0	29.0	41.0
27	35.86243128568054	38.0	34.0	40.0	29.0	41.0
28	35.6672980918797	38.0	34.0	40.0	27.0	41.0
29	35.427369269734754	38.0	34.0	40.0	27.0	41.0
30	35.228223566873794	37.0	34.0	40.0	27.0	41.0
31	34.86837797558453	37.0	33.0	40.0	26.0	41.0
32	34.65256981374856	37.0	33.0	40.0	25.0	41.0
33	34.40834263097458	36.0	33.0	40.0	25.0	41.0
34	34.14427848946584	36.0	33.0	39.0	24.0	40.0
35	33.872224348690054	36.0	33.0	39.0	24.0	40.0
36	33.74743507260304	36.0	33.0	39.0	22.0	40.0
37	33.526927054914445	36.0	32.0	39.0	21.0	40.0
38	33.31724312042414	36.0	32.0	39.0	20.0	40.0
39	33.06756358365027	36.0	31.0	39.0	18.0	40.0
40	32.80120937202238	36.0	31.0	39.0	17.0	40.0
41	32.56765642433077	36.0	31.0	39.0	15.0	40.0
42	32.18277153863068	35.0	30.0	39.0	14.0	40.0
43	31.708093427042698	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	0.0
13	2.0
14	9.0
15	16.0
16	49.0
17	102.0
18	293.0
19	478.0
20	1217.0
21	2041.0
22	3686.0
23	5762.0
24	8797.0
25	12999.0
26	17987.0
27	24183.0
28	31129.0
29	39379.0
30	47709.0
31	56894.0
32	68215.0
33	82003.0
34	98328.0
35	121669.0
36	158883.0
37	195413.0
38	180224.0
39	70439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.03966903111792	25.274409362249678	17.07918332776832	18.606738278864086
2	22.711273627545992	27.094575335325878	30.928406805059	19.265744232069125
3	23.50807469602821	24.843270272251225	33.551726103704674	18.096928928015895
4	17.313483887255583	19.443770308898863	32.35921199436441	30.88353380948115
5	11.076056062740754	42.56427588341165	33.28688584668257	13.072782207165021
6	31.316220244154703	39.09773517603082	12.33925939197498	17.2467851878395
7	22.939303369139434	36.74349097246541	20.587258023796533	19.729947634598627
8	28.50103020579684	35.61954866399003	17.844304550007735	18.03511658020539
9	24.22661269962782	14.206741536431824	22.41491640266795	39.15172936127241
10	16.886823952895572	31.03378912135254	31.146989600214997	20.93239732553689
11	33.57680937527995	23.063009503953875	22.464431432271095	20.895749688495087
12	19.981757620672525	30.69076723864127	31.052601575034	18.274873565652207
13	33.63788877034962	21.549380654933994	26.48581736446482	18.326913210251565
14	19.160199037388733	24.672166526862718	30.51901197970535	25.648622456043196
15	22.05120896482641	32.020262071324446	26.578413727390444	19.3501152364587
16	19.412741976203467	29.10457606827862	28.89690612504174	22.585775830476177
17	20.527155899047976	28.45933333876261	29.062797762050963	21.950713000138446
18	21.419729458999438	25.38850567223982	32.151216294353816	21.04054857440692
19	21.827658378871416	25.01258235538435	34.652539681247	18.507219584497236
20	22.014642766978035	24.729906915001916	34.154376135058754	19.10107418296129
21	20.951209779218345	25.196146297367072	35.19354024317743	18.659103680237152
22	19.08902118233421	26.006792028731745	34.74220423320927	20.161982555724766
23	20.36126426203875	26.05451539607952	35.2275818260296	18.35663851585214
24	19.957733058611787	26.046452915930317	33.5667109152951	20.429103110162796
25	20.005945061120116	26.451205707258673	34.28997239211343	19.25287683950778
26	19.2492120758036	27.917844141671623	34.03254310169312	18.800400680831657
27	18.56153952651253	27.35322621364758	33.89450366883566	20.19073059100423
28	19.038773199990228	27.848213631292197	33.759558925328406	19.353454243389173
29	19.07542083703203	26.664413515648537	33.025628914171236	21.234536733148197
30	19.439128274873564	27.39394581036069	34.699204339080225	18.467721575685513
31	20.242525918023308	27.355750828643792	33.9353047047422	18.466418548590692
32	19.474391445627123	27.216408368691518	33.58242867962636	19.726771506055005
33	19.134464252266046	27.140181283644566	34.071633914537706	19.653720549551675
34	18.389377071609484	27.535975763696037	34.69798275117883	19.37666441351565
35	17.75936347126418	29.414533638458845	33.75125212759893	19.074850762678047
36	20.583756138479202	27.060533752473713	33.01406454870471	19.34164556034237
37	19.391404907525796	28.409411113192334	32.836934303002664	19.362249676279205
38	19.40231775944491	26.20004723473219	34.25381339023218	20.143821615590717
39	18.470246190681728	26.307791287635084	34.933586337760914	20.288376183922274
40	17.591028658452167	26.47246133674292	34.52313280289272	21.413377201912194
41	18.641431375263657	25.73388929156046	34.745706118526606	20.87897321464928
42	18.398009626112664	27.241735957847073	33.410103346336456	20.950151069703807
43	18.396462281437564	27.174385744883583	32.84361231686361	21.58553965681524
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	586.0
1	486.5
2	387.0
3	640.5
4	894.0
5	894.0
6	1712.0
7	2530.0
8	2963.5
9	3397.0
10	5352.0
11	7307.0
12	7307.0
13	14726.5
14	22146.0
15	40348.5
16	58551.0
17	51309.0
18	44067.0
19	44067.0
20	47762.5
21	51458.0
22	39367.0
23	27276.0
24	28356.0
25	29436.0
26	29436.0
27	30721.5
28	32007.0
29	33030.5
30	34054.0
31	36448.5
32	38843.0
33	38843.0
34	41994.5
35	45146.0
36	48677.5
37	52209.0
38	54488.0
39	56767.0
40	56767.0
41	58834.0
42	60901.0
43	62711.0
44	64521.0
45	69921.5
46	75322.0
47	75322.0
48	102954.5
49	130587.0
50	118328.0
51	106069.0
52	91797.0
53	77525.0
54	77525.0
55	67438.5
56	57352.0
57	49672.0
58	41992.0
59	37005.0
60	32018.0
61	32018.0
62	27912.5
63	23807.0
64	20366.0
65	16925.0
66	14338.0
67	11751.0
68	11751.0
69	9896.5
70	8042.0
71	6744.0
72	5446.0
73	4559.0
74	3672.0
75	3672.0
76	2919.0
77	2166.0
78	1704.5
79	1243.0
80	966.0
81	689.0
82	689.0
83	544.0
84	399.0
85	307.0
86	215.0
87	161.0
88	107.0
89	107.0
90	80.0
91	53.0
92	42.5
93	32.0
94	21.0
95	10.0
96	10.0
97	6.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1227910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.821811849800426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.7623877900588	44.24481087936507
2	9.412211272950207	9.943401059006929
3	2.7201005310072106	4.310419153842155
4	1.184848668512408	2.503434137545959
5	0.6409130022107475	1.692709300743342
6	0.4225928958184019	1.3393273459189166
7	0.27649933399468535	1.0223637057803678
8	0.20250424302530012	0.8557312819094932
9	0.1593009671996158	0.7573109145218402
>10	1.0589449819175887	10.565439218709525
>50	0.09521347459768191	3.478259163629391
>100	0.053322350169980666	5.184723026939653
>500	0.005890257839196396	2.1916940412343417
>1k	0.0044951967720183025	4.620750643195609
>5k	3.1001357048402085E-4	1.1879590871289998
>10k+	4.650203557260313E-4	6.1016670405284525
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31814	2.5909064996620277	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25182	2.050801768859281	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17526	1.4273033039880774	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9111	0.741992491306366	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5398	0.4396087661147804	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4888	0.398074777467404	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3376	0.2749387170069467	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3189	0.2597095878362421	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3173	0.25840656074142243	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3090	0.25164710768704546	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2924	0.23812820157829157	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2426	0.19757148325202986	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2419	0.19700140889804627	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2241	0.18250523246817765	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	2231	0.18169084053391538	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	2152	0.17525714425324332	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2000	0.1628783868524566	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1778	0.14479888591183393	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1751	0.14260002768932575	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1488	0.12118151981822772	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1469	0.11963417514312938	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1412	0.11499214111783437	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1394	0.11352623563616226	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1350	0.10994291112540822	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1336	0.10880276241744102	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1240	0.10098459984852311	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1235	0.10057740388139196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	8.143919342622831E-5	0.0	0.0	0.0	0.0
10	8.143919342622831E-5	0.0	0.0	1.6287838685245663E-4	0.0
11	8.143919342622831E-5	0.0	0.0	1.6287838685245663E-4	0.0
12	8.143919342622831E-5	0.0	0.0	3.2575677370491325E-4	0.0
13	8.143919342622831E-5	0.0	0.0	4.886351605573699E-4	0.0
14	2.4431758027868494E-4	0.0	0.0	4.886351605573699E-4	0.0
15	2.4431758027868494E-4	0.0	0.0	8.14391934262283E-4	0.0
16	3.2575677370491325E-4	0.0	0.0	9.772703211147398E-4	0.0
17	3.2575677370491325E-4	0.0	0.0	0.001303027094819653	0.0
18	3.2575677370491325E-4	0.0	0.0	0.0017102230619507944	0.0
19	3.2575677370491325E-4	0.0	0.0	0.0020359798356557077	0.0
20	3.2575677370491325E-4	0.0	0.0	0.003909081284458959	0.0
21	3.2575677370491325E-4	0.0	0.0	0.006433696280672036	0.0
22	3.2575677370491325E-4	0.0	0.0	0.011727243853376876	0.0
23	3.2575677370491325E-4	0.0	0.0	0.014170419656163726	0.0
24	3.2575677370491325E-4	0.0	0.0	0.017102230619507944	0.0
25	3.2575677370491325E-4	0.0	0.0	0.019952602389425936	0.0
26	3.2575677370491325E-4	0.0	0.0	0.02557190673583569	0.0
27	3.2575677370491325E-4	0.0	0.0	0.07085209828081862	0.0
28	3.2575677370491325E-4	0.0	0.0	0.17818895521658754	0.0
29	3.2575677370491325E-4	0.0	0.0	0.3475824775431424	0.0
30	3.2575677370491325E-4	0.0	0.0	0.5824531113843848	0.0
31	3.2575677370491325E-4	0.0	0.0	0.8656171869273807	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6720	0.0	26.456102	1
TAGACTG	105	4.0017767E-11	22.904764	5
ACGGTGT	95	1.6763443E-7	19.473684	29
TCGAACG	50	0.0070351497	18.5	26
GATAGAC	95	3.6067922E-6	17.526316	3
TATGCGG	85	2.72336E-5	17.411764	5
GTATCAA	10270	0.0	17.3111	2
GACCCTC	345	0.0	17.15942	7
ATAGACA	165	5.4569682E-11	16.818182	4
CGGTGTG	110	7.808667E-7	16.818182	30
GGACCCT	365	0.0	16.726027	6
ACCGTGA	135	2.2195309E-8	16.444445	8
GAACTAG	80	3.382801E-4	16.1875	6
CTAGGAC	80	3.382801E-4	16.1875	3
TACCGTG	115	1.2422879E-6	16.086956	7
CTAGACT	105	9.344056E-6	15.857144	4
GTATTGG	190	2.7284841E-11	15.578948	1
TTATGCG	95	7.0603004E-5	15.578948	4
GCCGATA	95	7.0603004E-5	15.578948	2
TAGGAAC	215	0.0	15.488372	30
>>END_MODULE