FastQCFastQC Report
Wed 25 May 2016
SRR2088501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448199
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104832.3389164188228886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81711.8230741255558358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57051.2728720947614787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.6876409809035718No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG17260.38509679852030015No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA15450.3447129511667808No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA12260.2735392091459374No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG11540.25747491627602914No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG10720.23917947161863368No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.23137044036242832No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9750.2175372992800073No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA9430.2103976135600481No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT9010.20102677605260164No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC7810.17425295460275458No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.15372635815787183No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT6410.14301682957793302No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT6210.1385545260029585No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.12829122778051713No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG5580.12449826974178882No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG5490.12249023313305027No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA5470.12204400277555283No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA5200.11601989294933723No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA4770.10642594026314205No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.10620282508439331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA451.3214527E-424.6666684
GGTATCA27150.022.5543271
TGTGCTA502.6985392E-422.210
AATACTG502.6985392E-422.25
GTAACGA502.6985392E-422.23
TTGGAGT450.003822220520.5555574
AGTACCG450.003822220520.5555575
TATACTG759.245099E-619.7333345
GGACCCT1254.0927262E-1019.2400026
GGCGTCG500.0070283518.58
GACCCTC1205.14774E-918.57
ACGGCTT500.0070283518.515
AAATTCG500.0070283518.526
TGCTAGG500.0070283518.52
TAATCCT500.0070283518.54
ACAGTGC1054.781141E-717.6190478
ATTGGAC1054.781141E-717.6190473
TTGGACC1603.274181E-1117.343754
TGGACCC1401.8590072E-917.1785725
ATATAAC650.001577901717.0769233
Produced by FastQC (version 0.11.5)