##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088501_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448199
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96549300645472	31.0	31.0	33.0	30.0	34.0
2	31.10689225098673	31.0	31.0	34.0	28.0	34.0
3	31.105616032164285	31.0	31.0	34.0	28.0	34.0
4	34.895611101318835	35.0	35.0	37.0	32.0	37.0
5	34.7266147403274	35.0	35.0	37.0	32.0	37.0
6	34.94448225007196	35.0	35.0	37.0	32.0	37.0
7	34.76394637203563	35.0	35.0	37.0	32.0	37.0
8	34.90643664979172	35.0	35.0	37.0	32.0	37.0
9	36.35470181771936	38.0	35.0	39.0	32.0	39.0
10	36.0938801737621	38.0	35.0	39.0	31.0	39.0
11	36.26725628571237	38.0	35.0	39.0	32.0	39.0
12	36.000899154170355	37.0	35.0	39.0	31.0	39.0
13	36.155692002882645	38.0	35.0	39.0	32.0	39.0
14	36.84684035439615	38.0	36.0	40.0	31.0	41.0
15	36.91679588754103	38.0	36.0	40.0	31.0	41.0
16	36.729147097606194	38.0	36.0	40.0	31.0	41.0
17	36.83763685327276	38.0	36.0	40.0	31.0	41.0
18	36.85682029634158	38.0	36.0	40.0	31.0	41.0
19	36.93668437457469	39.0	36.0	40.0	31.0	41.0
20	36.826164270781504	39.0	36.0	40.0	31.0	41.0
21	36.68567979848237	39.0	35.0	40.0	30.0	41.0
22	36.534289456246	38.0	35.0	40.0	30.0	41.0
23	36.335148003453824	38.0	35.0	40.0	30.0	41.0
24	36.465449498994865	38.0	35.0	40.0	30.0	41.0
25	36.3896974335061	38.0	35.0	40.0	30.0	41.0
26	36.08080116198385	38.0	35.0	40.0	29.0	41.0
27	36.01456942117229	38.0	35.0	40.0	29.0	41.0
28	35.82053953712525	38.0	34.0	40.0	29.0	41.0
29	35.5876385266366	38.0	34.0	40.0	27.0	41.0
30	35.38627038436052	38.0	34.0	40.0	27.0	41.0
31	35.03533698201022	37.0	34.0	40.0	26.0	41.0
32	34.84846017059387	37.0	33.0	40.0	26.0	41.0
33	34.61887688281321	37.0	33.0	40.0	25.0	41.0
34	34.36460590050402	37.0	33.0	40.0	24.0	41.0
35	34.12016537297049	36.0	33.0	40.0	24.0	41.0
36	34.02881978763897	37.0	33.0	40.0	23.0	41.0
37	33.82890189402475	37.0	33.0	40.0	22.0	41.0
38	33.6372281062653	36.0	33.0	40.0	21.0	41.0
39	33.418410125859275	36.0	32.0	40.0	20.0	41.0
40	33.16345864225489	36.0	31.0	40.0	18.0	41.0
41	32.942654936758004	36.0	31.0	39.0	17.0	41.0
42	32.579086521835166	36.0	31.0	39.0	15.0	41.0
43	32.11024120981975	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	3.0
15	4.0
16	24.0
17	43.0
18	75.0
19	197.0
20	369.0
21	726.0
22	1275.0
23	1883.0
24	3000.0
25	4426.0
26	6115.0
27	8240.0
28	10755.0
29	13655.0
30	16599.0
31	19708.0
32	24028.0
33	28829.0
34	34876.0
35	43247.0
36	56313.0
37	71363.0
38	71034.0
39	31408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.05876630693063	24.846552535815565	16.59887683819018	19.49580431906363
2	22.089295156838816	26.46123708442009	31.571913368838395	19.8775543899027
3	22.947173019127664	24.754852197349837	33.14509849419566	19.15287628932684
4	16.91012251254465	18.801916113155094	32.87780651005468	31.41015486424557
5	11.562944138652696	41.714060049219206	33.52841037128597	13.194585440842127
6	31.92242731465264	38.63239320034182	12.381999959839268	17.063179525166277
7	23.865961325214915	35.881606161548774	20.617404322633472	19.635028190602835
8	28.505641467294662	35.33341216736316	18.026367751824523	18.134578613517657
9	24.33227204879975	14.339166307823087	21.90053971561739	39.428021927759765
10	16.967239998304326	30.237238369563517	31.436705570516665	21.35881606161549
11	33.446973331042685	22.946726788770167	22.382022271357144	21.224277608830004
12	20.43221872427203	30.09377530962809	30.711135009225814	18.762870956874067
13	33.71515777589865	21.130792348934293	25.865519557160994	19.288530318006064
14	19.400087907380428	24.279839981793803	29.73969152095386	26.58038058987191
15	22.572562634008555	31.467718580362742	25.94695659740428	20.01276218822443
16	20.218251267852004	28.5951106539729	28.17855461524903	23.008083462926066
17	21.078137166749592	28.154904406301668	28.45254005475247	22.31441837219628
18	21.9344532227872	25.444724330040895	31.21091300962296	21.409909437548947
19	22.126332276511103	25.102242530661606	33.618325788321705	19.15309940450559
20	22.777159252921138	24.71982311428629	32.687935492939516	19.815082139853057
21	21.472158572419843	25.347446112106454	33.79681793132068	19.38357738415302
22	19.892056876521366	26.06966994571608	33.09199708165346	20.946276096109095
23	20.852567721034628	26.189259681525396	33.635282541906605	19.322890055533367
24	20.610041521734765	26.328037322707097	32.1044893005116	20.95743185504653
25	20.690809216441806	26.31308860573094	32.753531355491646	20.242570822335615
26	20.002498890001984	27.70577355148048	32.87825274041219	19.41347481810535
27	19.46122146635758	27.09309927063648	32.596458269652544	20.8492209933534
28	19.667603006700148	27.59243104067613	32.69373648758699	20.046229465036735
29	20.01588580072691	26.57034040682822	31.686148340357747	21.727625452087132
30	19.867960437216503	27.381140966401084	33.59802230705557	19.15287628932684
31	21.019234759559925	27.236785445750662	32.806186537676346	18.93779325701307
32	20.135475536536227	26.972617074112172	32.03800097724449	20.853906412107122
33	19.81999067378553	27.056954611679185	32.87713716451844	20.245917550016845
34	19.21579476973398	27.377794238719854	33.34835642203575	20.058054569510418
35	18.430652455717215	29.31911940901251	32.62434766699614	19.625880468274136
36	21.22226957222127	27.12924392959377	31.80551496098831	19.842971537196647
37	19.83226200861671	28.430674767235093	31.669637817130337	20.067425407017865
38	20.004953156968224	26.29970169500601	33.27584398894241	20.419501159083353
39	19.15979285986805	26.439818027260216	33.73635371787978	20.664035394991956
40	18.092856075091646	26.604923259534267	33.41283670869413	21.889383956679957
41	19.366620630568118	25.912150629519477	33.55027565880334	21.17095308110906
42	18.827351243532448	27.475964917369293	32.431353037378486	21.265330801719774
43	18.831367316749926	27.479534760229274	31.965042313793653	21.724055609227154
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	270.0
1	199.0
2	128.0
3	230.0
4	332.0
5	332.0
6	592.0
7	852.0
8	956.5
9	1061.0
10	1776.5
11	2492.0
12	2492.0
13	4963.0
14	7434.0
15	13393.0
16	19352.0
17	16747.5
18	14143.0
19	14143.0
20	15302.5
21	16462.0
22	12496.0
23	8530.0
24	9007.0
25	9484.0
26	9484.0
27	10100.0
28	10716.0
29	11181.0
30	11646.0
31	12526.0
32	13406.0
33	13406.0
34	14738.5
35	16071.0
36	17468.0
37	18865.0
38	19554.0
39	20243.0
40	20243.0
41	21141.5
42	22040.0
43	23289.5
44	24539.0
45	26420.0
46	28301.0
47	28301.0
48	38085.0
49	47869.0
50	43931.0
51	39993.0
52	35649.0
53	31305.0
54	31305.0
55	27753.0
56	24201.0
57	20355.5
58	16510.0
59	14515.5
60	12521.0
61	12521.0
62	10962.5
63	9404.0
64	8067.5
65	6731.0
66	5669.0
67	4607.0
68	4607.0
69	3900.0
70	3193.0
71	2702.0
72	2211.0
73	1802.0
74	1393.0
75	1393.0
76	1109.0
77	825.0
78	656.5
79	488.0
80	375.5
81	263.0
82	263.0
83	197.0
84	131.0
85	109.0
86	87.0
87	66.5
88	46.0
89	46.0
90	43.0
91	40.0
92	25.0
93	10.0
94	6.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	448199.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.818843410054846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.22661483084096	53.050218285860836
2	7.208272677217134	8.767976144252913
3	2.1141035544713067	3.8573199909609217
4	0.9872601364123088	2.4017607856579835
5	0.5771309485925284	1.75502183947727
6	0.3708724098853062	1.3533618613154466
7	0.250217049792935	1.0652538098917685
8	0.19519137486218377	0.9497050930189176
9	0.14085850309589681	0.7710166118487667
>10	0.8273077271471951	9.103509970144016
>50	0.05623068531488809	2.3335687552525153
>100	0.03785464435073142	4.8678601858147
>500	0.00441024982726968	1.8552318643705576
>1k	0.00257264573257398	2.4234245630729623
>5k	7.3504163787828E-4	3.1015900421693807
>10k+	3.6752081893914E-4	2.3431801968911516
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10483	2.3389164188228886	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8171	1.8230741255558358	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5705	1.2728720947614787	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3082	0.6876409809035718	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1726	0.38509679852030015	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1545	0.3447129511667808	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1226	0.2735392091459374	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1154	0.25747491627602914	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1072	0.23917947161863368	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1037	0.23137044036242832	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	975	0.2175372992800073	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	943	0.2103976135600481	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	901	0.20102677605260164	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	781	0.17425295460275458	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	689	0.15372635815787183	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	641	0.14301682957793302	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	621	0.1385545260029585	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	575	0.12829122778051713	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	558	0.12449826974178882	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	549	0.12249023313305027	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	547	0.12204400277555283	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	520	0.11601989294933723	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	477	0.10642594026314205	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	476	0.10620282508439331	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.2311517874872545E-4	0.0
11	0.0	0.0	0.0	2.2311517874872545E-4	0.0
12	0.0	0.0	0.0	8.924607149949018E-4	0.0
13	0.0	0.0	0.0	8.924607149949018E-4	0.0
14	0.0	0.0	0.0	0.0011155758937436272	0.0
15	0.0	0.0	0.0	0.0011155758937436272	0.0
16	0.0	0.0	0.0	0.0013386910724923528	0.0
17	0.0	0.0	0.0	0.00245426696623598	0.0
18	0.0	0.0	0.0	0.0026773821449847055	0.0
19	0.0	0.0	0.0	0.003569842859979607	0.0
20	0.0	0.0	0.0	0.006024109826215587	0.0
21	0.0	0.0	0.0	0.010263298222441371	0.0
22	0.0	0.0	0.0	0.01963413572988784	0.0
23	0.0	0.0	0.0	0.022980863411118723	0.0
24	0.0	0.0	0.0	0.028558742879836858	0.0
25	0.0	0.0	0.0	0.030566779488575387	0.0
26	0.0	0.0	0.0	0.0359215437785448	0.0
27	0.0	0.0	0.0	0.08009834917079243	0.0
28	0.0	0.0	0.0	0.2016961215888478	0.0
29	0.0	0.0	0.0	0.36434708689666867	0.0
30	0.0	0.0	0.0	0.6166903540614772	0.0
31	0.0	0.0	0.0	0.9096405837585537	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAA	45	1.3214527E-4	24.666668	4
GGTATCA	2715	0.0	22.554327	1
TGTGCTA	50	2.6985392E-4	22.2	10
AATACTG	50	2.6985392E-4	22.2	5
GTAACGA	50	2.6985392E-4	22.2	3
TTGGAGT	45	0.0038222205	20.555557	4
AGTACCG	45	0.0038222205	20.555557	5
TATACTG	75	9.245099E-6	19.733334	5
GGACCCT	125	4.0927262E-10	19.240002	6
GGCGTCG	50	0.00702835	18.5	8
GACCCTC	120	5.14774E-9	18.5	7
ACGGCTT	50	0.00702835	18.5	15
AAATTCG	50	0.00702835	18.5	26
TGCTAGG	50	0.00702835	18.5	2
TAATCCT	50	0.00702835	18.5	4
ACAGTGC	105	4.781141E-7	17.619047	8
ATTGGAC	105	4.781141E-7	17.619047	3
TTGGACC	160	3.274181E-11	17.34375	4
TGGACCC	140	1.8590072E-9	17.178572	5
ATATAAC	65	0.0015779017	17.076923	3
>>END_MODULE