FastQCFastQC Report
Wed 25 May 2016
SRR2088500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564540
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79481.4078718957026959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66151.1717504516951855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55550.9839869628370002No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30060.5324689127431184No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG20010.3544478690615368No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG18950.3356715201757183No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17260.30573564317851704No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16690.2956389272682184No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA16650.294930385800829No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG14760.26145180146668084No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA10110.17908385588266554No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA9130.16172458993162575No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC9020.15977610089630495No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA8850.1567647996599001No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.15056506182024304No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7470.13232011903496652No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.12328621532575194No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC6870.12169199702412584No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.11850356042087364No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA6310.11177241648067454No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG6280.11124101038013251No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG5910.10468700180678073No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC5790.10256137740461262No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG5780.10238424203776526No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT5730.10149856520352853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG358.8637834E-426.428575
TATCGCC358.8637834E-426.4285733
GGTATCA21250.023.9411771
ACTGAGC802.719571E-823.1258
TCTATCG400.001929976523.12531
CTAGCCT502.699743E-422.1999994
ACGGTGC603.7219645E-521.5833328
TTTGGAC1254.110916E-1019.243
CGAACTA609.2288817E-418.524
TTGGACC3300.018.54
TATGCTC701.2178034E-418.55
TCGCCTA500.00703055718.49999835
CCCTAGA500.00703055718.4999982
CGGAGTG500.00703055718.49999825
GTACGGA500.00703055718.4999981
TAATACT953.5998128E-617.5263164
TACACAG953.5998128E-617.5263165
TACACTA852.7191432E-517.4117645
ATTGGAC2350.017.3191473
GACCGGA650.001578587817.07692322
Produced by FastQC (version 0.11.5)