##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088500_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564540
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37341729549722	31.0	31.0	34.0	30.0	34.0
2	31.510936337549154	31.0	31.0	34.0	30.0	34.0
3	31.662838771389097	31.0	31.0	34.0	30.0	34.0
4	35.37062741346937	37.0	35.0	37.0	33.0	37.0
5	35.2540847415595	37.0	35.0	37.0	33.0	37.0
6	35.33592128104297	37.0	35.0	37.0	33.0	37.0
7	35.15795160661778	37.0	35.0	37.0	32.0	37.0
8	35.21298579374358	37.0	35.0	37.0	32.0	37.0
9	36.74985297764552	39.0	35.0	39.0	32.0	39.0
10	36.62274240974953	39.0	35.0	39.0	32.0	39.0
11	36.65313706734687	39.0	35.0	39.0	32.0	39.0
12	36.493881744429096	38.0	35.0	39.0	32.0	39.0
13	36.504573635172	38.0	35.0	39.0	32.0	39.0
14	37.410314592411524	39.0	36.0	41.0	32.0	41.0
15	37.45987352534807	39.0	36.0	41.0	32.0	41.0
16	37.31819534488256	39.0	36.0	41.0	32.0	41.0
17	37.41916250398555	39.0	36.0	41.0	32.0	41.0
18	37.29042051936089	39.0	36.0	40.0	32.0	41.0
19	37.38828603819039	39.0	36.0	41.0	32.0	41.0
20	37.30413079675488	39.0	36.0	41.0	31.0	41.0
21	37.13370531760371	39.0	36.0	40.0	31.0	41.0
22	37.04935876997201	39.0	36.0	40.0	31.0	41.0
23	36.8145746979842	39.0	35.0	40.0	30.0	41.0
24	37.01709710560811	39.0	36.0	40.0	31.0	41.0
25	36.978315088390545	39.0	36.0	41.0	31.0	41.0
26	36.77697063095618	39.0	35.0	40.0	30.0	41.0
27	36.716427533921426	39.0	35.0	40.0	30.0	41.0
28	36.525790909412976	39.0	35.0	40.0	30.0	41.0
29	36.33529776455167	38.0	35.0	40.0	30.0	41.0
30	36.11668083749601	38.0	35.0	40.0	30.0	41.0
31	35.81800403868636	38.0	35.0	40.0	28.0	41.0
32	35.677379813653594	38.0	34.0	40.0	28.0	41.0
33	35.45524852091969	38.0	34.0	40.0	27.0	41.0
34	35.32852233676976	38.0	34.0	40.0	27.0	41.0
35	35.06474651929004	38.0	33.0	40.0	25.0	41.0
36	34.903891664009635	38.0	33.0	40.0	25.0	41.0
37	34.70628122010841	38.0	33.0	40.0	24.0	41.0
38	34.59417756049173	38.0	33.0	40.0	24.0	41.0
39	34.455678959861125	38.0	33.0	40.0	23.0	41.0
40	34.29697275658058	38.0	33.0	40.0	22.0	41.0
41	34.0570287313565	38.0	33.0	40.0	21.0	41.0
42	33.77469621284585	37.0	33.0	40.0	19.0	41.0
43	33.43375314415276	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	1.0
16	5.0
17	20.0
18	46.0
19	133.0
20	287.0
21	519.0
22	1019.0
23	1695.0
24	2867.0
25	4110.0
26	5983.0
27	8344.0
28	11026.0
29	14333.0
30	17921.0
31	21883.0
32	26622.0
33	32520.0
34	39134.0
35	46989.0
36	59970.0
37	79347.0
38	96344.0
39	93418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47837177170794	21.438870584900982	14.199879547950545	22.882878095440535
2	18.986431430899493	24.16994367095334	34.62500442838417	22.218620469762993
3	19.863782902894393	24.648740567541715	33.10961136500514	22.377865164558756
4	13.787154143196231	16.36093102348815	36.101959117157335	33.74995571615828
5	12.15343465476317	39.10582066815461	34.91975767881815	13.820986998264074
6	32.19594714280653	37.32330747156977	13.148581145711551	17.33216423991214
7	25.637510185283595	33.56183795656641	21.175293159032133	19.625358699117864
8	27.694937471215503	34.12193998653771	18.696106564636693	19.48701597761009
9	24.490912955680734	14.423601516278739	21.91518758635349	39.17029794168704
10	16.515747334112728	27.739221312927338	33.09172069295355	22.653310660006376
11	33.00191306196195	22.48574060296879	23.624721011797213	20.887625323272044
12	21.195309455485884	27.53055585078117	30.862117830446028	20.412016863286926
13	31.993481418500018	21.006837425160306	26.271300527863396	20.72838062847628
14	20.3400999043469	23.36557197009955	27.807064158429874	28.487263967123678
15	22.26573847734439	29.987069118220145	25.87859141956283	21.86860098487264
16	21.55259149041698	28.700003542707336	26.61972579445212	23.127679172423566
17	21.63921068480533	26.881177595918803	26.85195026038899	24.62766145888688
18	23.203670244801078	25.481453927091085	29.60321677826195	21.711659049845892
19	22.716016579870338	24.730754242392035	30.820845289970595	21.732383887767032
20	23.85127714599497	24.283841711836185	30.73511177241648	21.129769369752367
21	22.666772947886777	25.16207886066532	31.36217097105608	20.80897722039182
22	21.05785241081234	25.603500194848905	30.928366457646934	22.41028093669182
23	22.18921599886633	25.9308463527828	31.91270769121763	19.96722995713324
24	22.09462571296985	26.00790023736139	29.206787827257592	22.690686222411166
25	21.84132213837815	26.34817727707514	30.511744074821976	21.298756509724733
26	21.80731214794346	27.201969745279342	30.220533531724946	20.770184575052255
27	20.40085733517554	27.27742941155631	29.905941120204062	22.415772133064088
28	21.07290891699437	26.74832607078329	29.96067594855989	22.21808906366245
29	21.4475502178765	26.544974669642542	29.3228114925426	22.684663619938355
30	21.411768873773337	26.6944769192617	31.320367024480106	20.573387182484854
31	22.156268820632725	26.503524993800266	31.066177773054164	20.27402841251284
32	21.660821199560704	26.458709745987885	29.84943493817976	22.031034116271655
33	21.239239026464023	26.381301590675594	30.622099408367877	21.75735997449251
34	20.276154036915013	27.217911928295603	31.137740461260492	21.36819357352889
35	19.84128671130478	29.48914160201226	29.97803521451093	20.691536472172032
36	22.529315903213234	26.795798349098384	29.70577815566656	20.969107592021825
37	21.32142983668119	27.717433662805114	29.560172884118042	21.40096361639565
38	21.130655046586604	25.966627696885958	31.231090799589044	21.67162645693839
39	20.551599532362633	25.792149360541327	31.8912743118291	21.764976795266943
40	19.39171715024622	26.49697098522691	31.45711552768626	22.654196336840613
41	21.011620080065185	25.31317532858611	31.493959683990507	22.181244907358202
42	19.724731639919227	27.36351719984412	30.954759627307187	21.956991532929464
43	20.0699684699047	27.230311403974916	29.97148120593758	22.728238920182804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	64.5
2	100.0
3	266.5
4	433.0
5	433.0
6	717.0
7	1001.0
8	1116.0
9	1231.0
10	1916.0
11	2601.0
12	2601.0
13	4989.0
14	7377.0
15	11778.5
16	16180.0
17	14768.0
18	13356.0
19	13356.0
20	13990.5
21	14625.0
22	11457.5
23	8290.0
24	9245.5
25	10201.0
26	10201.0
27	11004.5
28	11808.0
29	12601.5
30	13395.0
31	14954.5
32	16514.0
33	16514.0
34	18061.0
35	19608.0
36	21441.5
37	23275.0
38	24770.0
39	26265.0
40	26265.0
41	27414.5
42	28564.0
43	29996.0
44	31428.0
45	34331.5
46	37235.0
47	37235.0
48	49924.0
49	62613.0
50	58374.0
51	54135.0
52	49277.5
53	44420.0
54	44420.0
55	38763.0
56	33106.0
57	28183.5
58	23261.0
59	20482.0
60	17703.0
61	17703.0
62	15640.0
63	13577.0
64	11711.0
65	9845.0
66	8669.5
67	7494.0
68	7494.0
69	6435.5
70	5377.0
71	4545.5
72	3714.0
73	3061.5
74	2409.0
75	2409.0
76	1978.5
77	1548.0
78	1193.5
79	839.0
80	659.0
81	479.0
82	479.0
83	366.5
84	254.0
85	186.0
86	118.0
87	97.5
88	77.0
89	77.0
90	54.0
91	31.0
92	24.0
93	17.0
94	11.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	564540.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.669186924288155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.35863982022032	52.99977648704509
2	7.1712806184537525	8.701515286549908
3	2.0105561958833147	3.6593642900949153
4	0.8984322055565673	2.1802860567084745
5	0.5679440886560099	1.7228353038607973
6	0.35734973112074325	1.300807058082509
7	0.26914737470960365	1.143026667050686
8	0.19143412634627688	0.9291322235992053
9	0.1490350281730907	0.8137650584249811
>10	0.9067204761556101	10.440851834006354
>50	0.06985641320675269	2.9410497468790058
>100	0.04138552031968267	4.963778090568473
>500	0.004989743584642529	2.0483709193115254
>1k	0.002348114628067073	2.5729732602914224
>5k	8.805429855251523E-4	3.582467717526668
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7948	1.4078718957026959	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6615	1.1717504516951855	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5555	0.9839869628370002	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3006	0.5324689127431184	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2001	0.3544478690615368	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1895	0.3356715201757183	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1726	0.30573564317851704	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1669	0.2956389272682184	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1665	0.294930385800829	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1476	0.26145180146668084	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1011	0.17908385588266554	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	913	0.16172458993162575	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	902	0.15977610089630495	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	885	0.1567647996599001	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	850	0.15056506182024304	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	747	0.13232011903496652	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	696	0.12328621532575194	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	687	0.12169199702412584	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	669	0.11850356042087364	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	631	0.11177241648067454	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	628	0.11124101038013251	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	591	0.10468700180678073	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	579	0.10256137740461262	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	578	0.10238424203776526	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	573	0.10149856520352853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.7713536684734474E-4	0.0
10	0.0	0.0	0.0	1.7713536684734474E-4	0.0
11	3.542707336946895E-4	0.0	0.0	3.542707336946895E-4	0.0
12	3.542707336946895E-4	0.0	0.0	0.0010628122010840685	0.0
13	3.542707336946895E-4	0.0	0.0	0.0010628122010840685	0.0
14	3.542707336946895E-4	0.0	0.0	0.0012399475679314132	0.0
15	3.542707336946895E-4	0.0	0.0	0.0019484890353207922	0.0
16	3.542707336946895E-4	0.0	0.0	0.003542707336946895	0.0
17	3.542707336946895E-4	0.0	0.0	0.004959790271725653	0.0
18	3.542707336946895E-4	0.0	0.0	0.0061997378396570655	0.0
19	3.542707336946895E-4	0.0	0.0	0.007971091508130513	0.0
20	3.542707336946895E-4	0.0	0.0	0.012930881779856166	0.0
21	3.542707336946895E-4	0.0	0.0	0.02090197328798668	0.0
22	3.542707336946895E-4	0.0	0.0	0.031707230665674706	0.0
23	3.542707336946895E-4	0.0	0.0	0.03772983313848443	0.0
24	3.542707336946895E-4	0.0	0.0	0.044283841711836185	1.7713536684734474E-4
25	3.542707336946895E-4	0.0	0.0	0.04924363198356184	1.7713536684734474E-4
26	3.542707336946895E-4	0.0	0.0	0.06022602472809721	1.7713536684734474E-4
27	3.542707336946895E-4	0.0	0.0	0.11832642505402628	1.7713536684734474E-4
28	3.542707336946895E-4	0.0	0.0	0.2324016013037163	1.7713536684734474E-4
29	3.542707336946895E-4	0.0	0.0	0.37995536188755447	1.7713536684734474E-4
30	3.542707336946895E-4	0.0	0.0	0.6019059765472774	1.7713536684734474E-4
31	3.542707336946895E-4	0.0	0.0	0.802068941084777	3.542707336946895E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCG	35	8.8637834E-4	26.42857	5
TATCGCC	35	8.8637834E-4	26.42857	33
GGTATCA	2125	0.0	23.941177	1
ACTGAGC	80	2.719571E-8	23.125	8
TCTATCG	40	0.0019299765	23.125	31
CTAGCCT	50	2.699743E-4	22.199999	4
ACGGTGC	60	3.7219645E-5	21.583332	8
TTTGGAC	125	4.110916E-10	19.24	3
CGAACTA	60	9.2288817E-4	18.5	24
TTGGACC	330	0.0	18.5	4
TATGCTC	70	1.2178034E-4	18.5	5
TCGCCTA	50	0.007030557	18.499998	35
CCCTAGA	50	0.007030557	18.499998	2
CGGAGTG	50	0.007030557	18.499998	25
GTACGGA	50	0.007030557	18.499998	1
TAATACT	95	3.5998128E-6	17.526316	4
TACACAG	95	3.5998128E-6	17.526316	5
TACACTA	85	2.7191432E-5	17.411764	5
ATTGGAC	235	0.0	17.319147	3
GACCGGA	65	0.0015785878	17.076923	22
>>END_MODULE