FastQCFastQC Report
Wed 25 May 2016
SRR2088499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758530
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT193171.0984742938704486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160570.9130921849499297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT132510.7535270936520845No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71380.40590720658732005No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG46830.2663019681211012No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG43700.248503011037628No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA40510.23036285988865698No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38690.22001330656855442No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA38080.21654450023599253No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG32400.1842447953688592No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21900.12453583390672893No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA20410.11606284794686472No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.11583538523653278No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19070.10844284715074523No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA18680.10622508572500895No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.10110717474254063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.024.1655121
TATACCG1002.8770773E-718.55
TGTAACG500.007036327418.52
TAATACT6200.017.604844
AAGGTCG852.7244441E-517.4117645
ACGTTCG2450.017.36734622
GTATTGG6450.017.2093031
TTCGTTA2400.016.95833230
ATACTGG5650.016.0442496
CGTTCGC2550.015.96078423
AATACTG7000.015.8571435
CTCTAAT4900.015.8571421
TCGAACC2003.6379788E-1215.72531
CGTTACC2600.015.65384532
CTAATAC5100.015.5980393
CGCCACG3000.015.41666718
TCTAATA5200.015.2980762
GTAAGAT2800.015.1964293
TTGGACC10350.015.1932374
ATAAGAC1353.9753286E-715.0740753
Produced by FastQC (version 0.11.5)