##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1758530
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.273581912165273	31.0	31.0	33.0	30.0	34.0
2	31.413471479019407	31.0	31.0	34.0	30.0	34.0
3	31.561511034784736	31.0	31.0	34.0	30.0	34.0
4	35.28982843625073	37.0	35.0	37.0	33.0	37.0
5	35.14820219160321	37.0	35.0	37.0	32.0	37.0
6	35.22713743865615	37.0	35.0	37.0	32.0	37.0
7	35.057120435818554	37.0	35.0	37.0	32.0	37.0
8	35.1014233479099	37.0	35.0	37.0	32.0	37.0
9	36.63296503329485	39.0	35.0	39.0	32.0	39.0
10	36.46584419941656	38.0	35.0	39.0	32.0	39.0
11	36.508173872495775	38.0	35.0	39.0	32.0	39.0
12	36.34124979386192	38.0	35.0	39.0	32.0	39.0
13	36.366295712896566	38.0	35.0	39.0	32.0	39.0
14	37.2897397257937	39.0	36.0	40.0	32.0	41.0
15	37.31305806554338	39.0	36.0	40.0	32.0	41.0
16	37.17787583947957	39.0	36.0	40.0	31.0	41.0
17	37.24565005999329	39.0	36.0	40.0	32.0	41.0
18	37.11277487446902	39.0	36.0	40.0	31.0	41.0
19	37.20568770507185	39.0	36.0	40.0	31.0	41.0
20	37.108044787407664	39.0	36.0	40.0	31.0	41.0
21	36.94061801618397	39.0	36.0	40.0	31.0	41.0
22	36.8427442238688	39.0	36.0	40.0	30.0	41.0
23	36.643660898591435	39.0	35.0	40.0	30.0	41.0
24	36.84981433356269	39.0	36.0	40.0	30.0	41.0
25	36.79937788948724	39.0	35.0	40.0	30.0	41.0
26	36.59969235668428	39.0	35.0	40.0	30.0	41.0
27	36.544153355359306	39.0	35.0	40.0	30.0	41.0
28	36.353532780219844	38.0	35.0	40.0	30.0	41.0
29	36.18476966557295	38.0	35.0	40.0	29.0	41.0
30	36.000445258255475	38.0	35.0	40.0	29.0	41.0
31	35.735629758946395	38.0	34.0	40.0	27.0	41.0
32	35.599520053681196	38.0	34.0	40.0	27.0	41.0
33	35.40952158905449	38.0	34.0	40.0	27.0	41.0
34	35.288343104752265	38.0	34.0	40.0	26.0	41.0
35	35.03661865307956	38.0	33.0	40.0	25.0	41.0
36	34.88732634643708	38.0	33.0	40.0	25.0	41.0
37	34.72857443432867	38.0	33.0	40.0	24.0	41.0
38	34.6430689268878	38.0	33.0	40.0	24.0	41.0
39	34.55005032612466	38.0	33.0	40.0	24.0	41.0
40	34.428803034352555	38.0	33.0	40.0	23.0	41.0
41	34.22631800424218	38.0	33.0	40.0	22.0	41.0
42	33.99557869356792	38.0	33.0	40.0	21.0	41.0
43	33.662686448340374	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	2.0
13	1.0
14	4.0
15	13.0
16	30.0
17	71.0
18	202.0
19	439.0
20	898.0
21	1924.0
22	3489.0
23	5785.0
24	9112.0
25	13715.0
26	19550.0
27	27234.0
28	35817.0
29	45866.0
30	57552.0
31	69951.0
32	84774.0
33	102536.0
34	122964.0
35	148795.0
36	185242.0
37	241333.0
38	309061.0
39	272166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.076581008001	21.703525103353368	13.700932028455584	23.518961860190046
2	19.356223664083068	23.87266637475619	34.2745929839127	22.496516977248042
3	19.647262201952767	24.958573353880798	32.500952500099515	22.89321194406692
4	14.078406396251413	16.740061301200434	36.271999909014916	32.90953239353323
5	12.385344577573314	39.01679243459025	35.06235321546974	13.535509772366694
6	32.31295457001018	37.9647773993051	13.556720670105143	16.165547360579573
7	26.255451996838268	32.884966420817385	21.504495231812935	19.35508635053141
8	27.166895077138292	34.41982792445963	18.90306107942429	19.51021591897778
9	24.824825280205626	14.199985214923828	21.266682968160907	39.708506536709635
10	16.256873638777844	27.74823289906911	33.639460230988384	22.355433231164664
11	33.7466520332323	22.4304959255742	23.21927973932773	20.603572301865764
12	21.116330116631506	27.427851671566593	30.63837409654655	20.817444115255356
13	31.240467890795152	21.111610265392116	25.945647785366187	21.702274058446545
14	20.69819678936384	22.814339249259323	27.415227491143167	29.07223647023366
15	22.878426867895342	29.705037730377075	25.236703382939158	22.17983201878842
16	22.163340972289355	28.377849681267875	26.114652579142806	23.34415676729996
17	22.043070064201352	26.954331174333106	26.71708756745691	24.28551119400863
18	22.935121948445577	25.907320318675257	29.124780356320336	22.03277737655883
19	23.090365248247117	24.993375148561583	29.90537551250192	22.010884090689384
20	23.742045913348083	24.92371469352243	29.794885500958184	21.539353892171302
21	22.780617902452615	25.653017008524166	30.086890755346797	21.479474333676425
22	21.666221218858933	26.113572131268732	29.730342956901502	22.489863692970832
23	22.58517056859991	26.266199609901452	30.516852143551716	20.63177767794692
24	22.59660056979409	26.41052469960706	28.107169055972886	22.885705674625967
25	22.448920405111085	26.307086032083614	29.414397252250456	21.829596310554837
26	22.238858592119552	27.140281940029453	29.236692009803644	21.384167458047347
27	21.28772326886661	27.097973875907716	28.8696240610055	22.74467879422017
28	21.66690360698993	26.63013994643253	29.25682245966802	22.446133986909523
29	22.233911278169835	26.67415398088176	28.581826866758032	22.510107874190375
30	22.04016991464462	26.694682490489214	30.11805314666227	21.147094448203898
31	22.500781903066766	26.622974871057075	29.899347750678125	20.976895475198035
32	21.997861850522877	26.362075142306356	29.07627393334205	22.563789073828712
33	21.788311828629595	26.415415147879195	29.681381608502555	22.114891414988655
34	21.21277430581224	26.84696877505644	30.188168527122084	21.752088392009234
35	20.776842021461107	28.72342240393965	29.0012681046101	21.498467469989137
36	22.916071946455276	26.589424121283116	28.863653164859286	21.63085076740232
37	21.72968331504154	27.23445150210687	28.96737616077064	22.068489022080943
38	21.630225244948907	25.866377030815514	30.263060624498873	22.240337099736713
39	21.176550869191882	25.869334046049826	30.898136511745605	22.055978573012684
40	20.24708136909805	26.409159923345065	30.4958687085236	22.84788999903328
41	21.425281342939844	25.22186144109	30.685629474618004	22.667227741352153
42	20.25879569868015	27.01711088238472	30.20465957362115	22.519433845313984
43	20.711730820628592	26.503670679487985	29.59790279381074	23.186695706072687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	133.0
1	224.5
2	316.0
3	642.0
4	968.0
5	968.0
6	1628.0
7	2288.0
8	2616.5
9	2945.0
10	4587.0
11	6229.0
12	6229.0
13	12128.5
14	18028.0
15	28565.5
16	39103.0
17	35822.5
18	32542.0
19	32542.0
20	34774.0
21	37006.0
22	30037.5
23	23069.0
24	25908.0
25	28747.0
26	28747.0
27	32268.5
28	35790.0
29	39403.0
30	43016.0
31	48533.0
32	54050.0
33	54050.0
34	61268.5
35	68487.0
36	74656.5
37	80826.0
38	86483.0
39	92140.0
40	92140.0
41	95393.5
42	98647.0
43	104891.5
44	111136.0
45	115069.5
46	119003.0
47	119003.0
48	147137.5
49	175272.0
50	166524.5
51	157777.0
52	145609.0
53	133441.0
54	133441.0
55	119672.5
56	105904.0
57	91482.0
58	77060.0
59	68884.0
60	60708.0
61	60708.0
62	53774.0
63	46840.0
64	40715.5
65	34591.0
66	29582.5
67	24574.0
68	24574.0
69	21040.0
70	17506.0
71	14671.0
72	11836.0
73	9783.0
74	7730.0
75	7730.0
76	6188.5
77	4647.0
78	3706.5
79	2766.0
80	2168.5
81	1571.0
82	1571.0
83	1218.0
84	865.0
85	675.0
86	485.0
87	364.5
88	244.0
89	244.0
90	200.0
91	156.0
92	106.5
93	57.0
94	37.5
95	18.0
96	18.0
97	12.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1758530.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.494133021115864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.61977764744861	45.56787286316196
2	9.649485885216766	10.516807348287648
3	2.705937067353538	4.423730834853956
4	1.1557812046935183	2.5193317884749646
5	0.6320716024522173	1.7222096991450497
6	0.38194902329125024	1.2488388529511203
7	0.2720630431358876	1.0378087763943649
8	0.19337461316606194	0.8430225514222561
9	0.15635575689927822	0.766842427357787
>10	1.056182132906242	11.071441330181912
>50	0.0958827832394951	3.6112625154362
>100	0.07052420441932274	7.4376809111344935
>500	0.007251856125500571	2.7659989215346665
>1k	0.0029427821958553043	3.273437588990866
>5k	1.0509936413768943E-4	0.4088145833298364
>10k+	3.1529809241306834E-4	2.7848990073428546
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19317	1.0984742938704486	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16057	0.9130921849499297	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13251	0.7535270936520845	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7138	0.40590720658732005	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4683	0.2663019681211012	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4370	0.248503011037628	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4051	0.23036285988865698	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3869	0.22001330656855442	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3808	0.21654450023599253	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3240	0.1842447953688592	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2190	0.12453583390672893	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2041	0.11606284794686472	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2037	0.11583538523653278	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1907	0.10844284715074523	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1868	0.10622508572500895	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1778	0.10110717474254063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.686567758298124E-5	0.0	0.0	0.0	0.0
7	5.686567758298124E-5	0.0	0.0	0.0	0.0
8	5.686567758298124E-5	0.0	0.0	0.0	0.0
9	5.686567758298124E-5	0.0	0.0	2.843283879149062E-4	0.0
10	1.1373135516596248E-4	0.0	0.0	3.980597430808687E-4	0.0
11	1.1373135516596248E-4	0.0	0.0	6.255224534127936E-4	0.0
12	1.1373135516596248E-4	0.0	0.0	8.529851637447186E-4	0.0
13	1.1373135516596248E-4	0.0	0.0	8.529851637447186E-4	0.0
14	1.1373135516596248E-4	0.0	0.0	0.0010235821964936624	0.0
15	1.1373135516596248E-4	0.0	0.0	0.001194179229242606	0.0
16	1.1373135516596248E-4	0.0	0.0	0.0015922389723234747	0.0
17	1.1373135516596248E-4	0.0	0.0	0.0024452241360681934	0.0
18	1.1373135516596248E-4	0.0	0.0	0.003070746589480987	0.0
19	1.1373135516596248E-4	0.0	0.0	0.004037463108391668	0.0
20	1.1373135516596248E-4	0.0	0.0	0.006482687244459861	0.0
21	1.1373135516596248E-4	0.0	0.0	0.010292687642519605	0.0
22	1.1373135516596248E-4	0.0	0.0	0.016263583788732636	0.0
23	1.1373135516596248E-4	0.0	0.0	0.01973239012129449	0.0
24	1.1373135516596248E-4	0.0	0.0	0.02621507736575435	0.0
25	1.1373135516596248E-4	0.0	0.0	0.030821197249975833	0.0
26	1.1373135516596248E-4	0.0	0.0	0.03844119804609532	0.0
27	1.1373135516596248E-4	0.0	0.0	0.08410433714522926	0.0
28	1.1373135516596248E-4	0.0	0.0	0.1782738992226462	0.0
29	1.1373135516596248E-4	0.0	0.0	0.30081943441397074	0.0
30	1.1373135516596248E-4	0.0	0.0	0.46976736251300805	0.0
31	1.1373135516596248E-4	0.0	0.0	0.6598693226729143	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5045	0.0	24.165512	1
TATACCG	100	2.8770773E-7	18.5	5
TGTAACG	50	0.0070363274	18.5	2
TAATACT	620	0.0	17.60484	4
AAGGTCG	85	2.7244441E-5	17.411764	5
ACGTTCG	245	0.0	17.367346	22
GTATTGG	645	0.0	17.209303	1
TTCGTTA	240	0.0	16.958332	30
ATACTGG	565	0.0	16.044249	6
CGTTCGC	255	0.0	15.960784	23
AATACTG	700	0.0	15.857143	5
CTCTAAT	490	0.0	15.857142	1
TCGAACC	200	3.6379788E-12	15.725	31
CGTTACC	260	0.0	15.653845	32
CTAATAC	510	0.0	15.598039	3
CGCCACG	300	0.0	15.416667	18
TCTAATA	520	0.0	15.298076	2
GTAAGAT	280	0.0	15.196429	3
TTGGACC	1035	0.0	15.193237	4
ATAAGAC	135	3.9753286E-7	15.074075	3
>>END_MODULE