FastQCFastQC Report
Wed 25 May 2016
SRR2088498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626160
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT432071.6452539068449752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT355831.354944100892558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT297861.134203551954184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156250.5949751728759862No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG111350.4240031071983428No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG97030.36947482255460445No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA90540.3447619337740275No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA86110.3278931976726475No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA84550.32195296554665365No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG77250.29415572546988755No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA49520.18856429158924057No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA49270.187612331312639No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC47770.1819005696530295No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45610.17367563286319188No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45320.17257135894233405No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.15029548846985713No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA39000.14850580314984616No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG37100.14127090504767417No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.13734882870807566No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35240.13418832058975844No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC33450.12737228500929113No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG31180.11872848569774881No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA31140.11857617205349255No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30940.1178146038322113No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT29240.11134127395132055No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT28500.10852347153257988No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC26750.10185974959636886No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA26660.10151704389679227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117450.023.1860351
GTATTGG13150.021.9467681
ATTGGAC16000.017.6906243
TATTGGA17300.017.0028922
TTGGACC20250.016.9925944
TGGACCC19550.016.7493615
GGACCCT20200.016.6683186
CTAATAC5350.016.5981313
TAATACG1059.352167E-615.8571424
GACCCTC21700.015.2603687
TAATACT6000.015.1083344
GTATCAA181900.014.94035152
GTCGGGT2301.8189894E-1214.4782624
TTTTTAC17450.014.4183391
CTCTAAT5200.014.2307691
ATACTGG5400.014.0462956
GGTAAGA3300.014.0151512
TAGCGAA3300.014.01515110
GGCGTAT800.006301465413.8751
ACCGTTA4000.013.8749998
Produced by FastQC (version 0.11.5)