##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088498_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2626160
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325489688366282	31.0	31.0	34.0	30.0	34.0
2	31.449837024400647	31.0	31.0	34.0	30.0	34.0
3	31.593667560240046	31.0	31.0	34.0	30.0	34.0
4	35.29870419167149	37.0	35.0	37.0	33.0	37.0
5	35.18948426600055	37.0	35.0	37.0	33.0	37.0
6	35.27657911170682	37.0	35.0	37.0	32.0	37.0
7	35.08755559448015	37.0	35.0	37.0	32.0	37.0
8	35.160951731806136	37.0	35.0	37.0	32.0	37.0
9	36.67140387485911	39.0	35.0	39.0	32.0	39.0
10	36.548678679136074	38.0	35.0	39.0	32.0	39.0
11	36.577144195327016	38.0	35.0	39.0	32.0	39.0
12	36.41561367167271	38.0	35.0	39.0	32.0	39.0
13	36.44902519267676	38.0	35.0	39.0	32.0	39.0
14	37.31937886495872	39.0	36.0	41.0	32.0	41.0
15	37.35042267036281	39.0	36.0	41.0	32.0	41.0
16	37.20366009687148	39.0	36.0	40.0	32.0	41.0
17	37.33801405854936	39.0	36.0	40.0	32.0	41.0
18	37.22454762847656	39.0	36.0	40.0	31.0	41.0
19	37.33014058549365	39.0	36.0	40.0	31.0	41.0
20	37.236475690742374	39.0	36.0	40.0	31.0	41.0
21	37.07257478599933	39.0	36.0	40.0	31.0	41.0
22	36.9862582630152	39.0	36.0	40.0	31.0	41.0
23	36.772263685380935	39.0	35.0	40.0	30.0	41.0
24	36.9631762725805	39.0	36.0	40.0	31.0	41.0
25	36.92195753495598	39.0	36.0	40.0	30.0	41.0
26	36.701992262466874	39.0	35.0	40.0	30.0	41.0
27	36.62984357388735	39.0	35.0	40.0	30.0	41.0
28	36.417878575562796	38.0	35.0	40.0	30.0	41.0
29	36.23210314679989	38.0	35.0	40.0	30.0	41.0
30	36.016136107472505	38.0	35.0	40.0	29.0	41.0
31	35.691640265634994	38.0	34.0	40.0	28.0	41.0
32	35.54050743290584	38.0	34.0	40.0	27.0	41.0
33	35.31255597526427	38.0	34.0	40.0	27.0	41.0
34	35.18030165717245	38.0	34.0	40.0	26.0	41.0
35	34.904692021811314	38.0	33.0	40.0	25.0	41.0
36	34.72005627989155	38.0	33.0	40.0	24.0	41.0
37	34.52992696560758	37.0	33.0	40.0	24.0	41.0
38	34.385079736192765	37.0	33.0	40.0	23.0	41.0
39	34.24437201084473	37.0	33.0	40.0	23.0	41.0
40	34.05466460535535	37.0	33.0	40.0	21.0	41.0
41	33.80132589027325	37.0	33.0	40.0	20.0	41.0
42	33.49819203704268	37.0	32.0	40.0	18.0	41.0
43	33.139697124318396	37.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	5.0
14	10.0
15	20.0
16	39.0
17	118.0
18	262.0
19	684.0
20	1379.0
21	2809.0
22	5046.0
23	8636.0
24	13831.0
25	20876.0
26	29778.0
27	40732.0
28	54805.0
29	69898.0
30	86625.0
31	105307.0
32	127010.0
33	154211.0
34	184885.0
35	220717.0
36	277841.0
37	369377.0
38	440426.0
39	410829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72076339598501	21.355857982758096	14.403577847503579	22.519800773753314
2	19.178534438114966	24.265733999451673	34.60520303408779	21.95052852834557
3	20.07794650744814	24.710109056569287	33.24230054528285	21.96964389069973
4	13.763098973406038	16.305708715386725	35.8595439729491	34.07164833825814
5	12.053835257562373	39.26744752794956	34.6313629024888	14.04735431199927
6	32.27248149389222	37.062897919395624	12.847998598714472	17.816621987997685
7	25.62163006062083	33.58081761964237	20.802312121119808	19.995240198616994
8	27.97784598044293	33.77280896822737	18.35611691595333	19.89322813537637
9	24.35483748134158	14.283440460596461	21.99945928656289	39.362262771499076
10	16.692128430864837	27.778391263289365	32.66834465531422	22.861135650531576
11	32.763502604563314	22.3830231212112	23.677879489444663	21.17559478478082
12	21.57397112133305	27.630228165839092	30.533440460596463	20.262360252231396
13	32.55231973680202	20.777713467572426	26.39142321869193	20.27854357693362
14	20.43100953483413	23.360952874158468	28.01192615834526	28.196111432662136
15	22.2752612178999	29.628126237548358	26.25213239101959	21.844480153532153
16	21.420591281567003	28.40885551527706	26.834541688244435	23.336011514911505
17	21.612925335851585	26.464191062235354	26.940704298291042	24.98217930362202
18	23.2132086392299	24.992841258719956	30.147173058762604	21.64677704328754
19	22.674627593139792	24.150965668504583	31.520280561732722	21.654126176622903
20	23.915184147195905	23.715539037987025	31.401018978280078	20.968257836536996
21	22.52539830017973	24.787217838974016	32.172982605781826	20.514401255064428
22	20.702051664788133	25.266739269503763	31.611440277820087	22.41976878788802
23	22.094426843756665	25.553012763883387	32.744273006975966	19.60828738538398
24	21.98190513906236	25.562113504127698	29.703445334633077	22.752536022176866
25	21.699058701678496	26.059684101501812	31.054657751241354	21.186599445578334
26	21.59441922807445	26.813865111036645	30.992209157096294	20.599506503792608
27	20.11077009778536	26.941694336978706	30.55556401742468	22.391971547811256
28	20.858706247905687	26.309059615560358	30.687581868583784	22.144652267950164
29	21.2324077740884	26.269876930575442	29.841974594084135	22.655740701252018
30	21.177041764401256	26.507752764492643	32.024895665153686	20.29030980595242
31	22.122262162244493	26.269724616931185	31.61045023913242	19.9975629816919
32	21.59910287263533	26.06585280409419	30.325456179364547	22.00958814390593
33	21.093155024827123	26.16885490602248	31.12727328114053	21.61071678800987
34	20.095196027660158	27.10272793736863	31.690986078532912	21.1110899564383
35	19.557262314558137	29.703521491455202	30.253297590398148	20.48591860358851
36	22.451792731592896	26.68298961220946	30.131751302281657	20.733466353915983
37	21.281643159594235	27.715676120266853	29.900653425533857	21.10202729460505
38	21.00869710908703	25.826948853078257	31.6258339172023	21.538520120632406
39	20.53858104609011	25.556820604989795	32.43412434885917	21.47047400006093
40	19.128118621866147	26.49373990922107	31.777271758004083	22.600869710908704
41	21.086986322234747	25.183195235629206	31.803926645748927	21.92589179638712
42	19.528931976726476	27.54752185700795	31.09067992810796	21.832866238157614
43	19.892276175099767	27.42822219514424	30.137424985530203	22.54207664422579
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	244.0
1	408.5
2	573.0
3	1355.5
4	2138.0
5	2138.0
6	3501.0
7	4864.0
8	5554.5
9	6245.0
10	9675.5
11	13106.0
12	13106.0
13	25625.5
14	38145.0
15	61336.0
16	84527.0
17	76162.5
18	67798.0
19	67798.0
20	70579.5
21	73361.0
22	55750.5
23	38140.0
24	41362.0
25	44584.0
26	44584.0
27	48141.5
28	51699.0
29	55224.5
30	58750.0
31	64302.0
32	69854.0
33	69854.0
34	77257.5
35	84661.0
36	93659.0
37	102657.0
38	109241.0
39	115825.0
40	115825.0
41	120106.5
42	124388.0
43	133191.0
44	141994.0
45	156299.5
46	170605.0
47	170605.0
48	237832.5
49	305060.0
50	283288.0
51	261516.0
52	236292.0
53	211068.0
54	211068.0
55	185070.5
56	159073.0
57	133080.5
58	107088.0
59	94526.0
60	81964.0
61	81964.0
62	72074.5
63	62185.0
64	54051.0
65	45917.0
66	39638.5
67	33360.0
68	33360.0
69	28667.0
70	23974.0
71	20233.5
72	16493.0
73	13529.0
74	10565.0
75	10565.0
76	8310.0
77	6055.0
78	4781.5
79	3508.0
80	2678.0
81	1848.0
82	1848.0
83	1435.5
84	1023.0
85	811.5
86	600.0
87	464.0
88	328.0
89	328.0
90	281.0
91	234.0
92	155.5
93	77.0
94	59.5
95	42.0
96	42.0
97	29.0
98	16.0
99	12.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2626160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.725108296657126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1832390616874	35.4147894231406
2	11.480551958753603	10.26937859321308
3	3.772063954308387	5.06117906575078
4	1.6876271687941438	3.0191723155479577
5	0.8731798688289815	1.952653209791853
6	0.55907754562669	1.5002882264629784
7	0.37041550451351796	1.1596811487889582
8	0.2604378167163674	0.9318487645747577
9	0.2015389340662841	0.8112465586896658
>10	1.3521009010425113	11.758368683416455
>50	0.14582935242279496	4.487838355058433
>100	0.09873512955556951	8.612834044517252
>500	0.008159696892444704	2.38895743482362
>1k	0.006184191329010654	5.759919740464648
>5k	4.294577311812954E-4	1.6729002118044969
>10k+	4.294577311812954E-4	5.198944223954565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43207	1.6452539068449752	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35583	1.354944100892558	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29786	1.134203551954184	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15625	0.5949751728759862	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11135	0.4240031071983428	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9703	0.36947482255460445	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9054	0.3447619337740275	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8611	0.3278931976726475	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8455	0.32195296554665365	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7725	0.29415572546988755	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4952	0.18856429158924057	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4927	0.187612331312639	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4777	0.1819005696530295	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4561	0.17367563286319188	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4532	0.17257135894233405	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3947	0.15029548846985713	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3900	0.14850580314984616	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3710	0.14127090504767417	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3607	0.13734882870807566	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3524	0.13418832058975844	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3345	0.12737228500929113	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3118	0.11872848569774881	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3114	0.11857617205349255	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3094	0.1178146038322113	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2924	0.11134127395132055	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2850	0.10852347153257988	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2675	0.10185974959636886	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2666	0.10151704389679227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.8078411064063116E-5	0.0	0.0	3.8078411064063116E-5
6	0.0	3.8078411064063116E-5	0.0	0.0	7.615682212812623E-5
7	3.8078411064063116E-5	3.8078411064063116E-5	0.0	0.0	7.615682212812623E-5
8	3.8078411064063116E-5	3.8078411064063116E-5	0.0	0.0	7.615682212812623E-5
9	3.8078411064063116E-5	3.8078411064063116E-5	0.0	1.903920553203156E-4	1.1423523319218936E-4
10	3.8078411064063116E-5	3.8078411064063116E-5	0.0	3.046272885125049E-4	1.1423523319218936E-4
11	7.615682212812623E-5	7.615682212812623E-5	0.0	5.711761659609468E-4	1.1423523319218936E-4
12	7.615682212812623E-5	7.615682212812623E-5	0.0	8.377250434093886E-4	1.1423523319218936E-4
13	7.615682212812623E-5	7.615682212812623E-5	0.0	9.138818655375149E-4	1.1423523319218936E-4
14	7.615682212812623E-5	1.1423523319218936E-4	0.0	0.0011042739208578305	1.5231364425625246E-4
15	7.615682212812623E-5	1.1423523319218936E-4	0.0	0.0014469796204343984	1.5231364425625246E-4
16	7.615682212812623E-5	1.1423523319218936E-4	0.0	0.0020562341974594083	1.5231364425625246E-4
17	7.615682212812623E-5	1.1423523319218936E-4	0.0	0.003274743351509428	1.5231364425625246E-4
18	1.1423523319218936E-4	1.1423523319218936E-4	0.0	0.003960154750662565	1.5231364425625246E-4
19	1.1423523319218936E-4	1.1423523319218936E-4	0.0	0.00525482072684071	1.5231364425625246E-4
20	1.1423523319218936E-4	1.5231364425625246E-4	0.0	0.008491485667286075	1.5231364425625246E-4
21	1.1423523319218936E-4	1.5231364425625246E-4	0.0	0.015650226947329943	1.5231364425625246E-4
22	2.2847046638437872E-4	1.5231364425625246E-4	0.0	0.025322143357601974	1.5231364425625246E-4
23	2.2847046638437872E-4	1.5231364425625246E-4	0.0	0.030120023151673926	1.5231364425625246E-4
24	2.2847046638437872E-4	1.903920553203156E-4	0.0	0.03724068602065373	1.5231364425625246E-4
25	2.2847046638437872E-4	1.903920553203156E-4	0.0	0.042800134036006944	1.5231364425625246E-4
26	2.2847046638437872E-4	1.903920553203156E-4	0.0	0.053081305023303986	1.903920553203156E-4
27	2.2847046638437872E-4	1.903920553203156E-4	0.0	0.11038931367471898	1.903920553203156E-4
28	2.2847046638437872E-4	1.903920553203156E-4	0.0	0.22340603771285833	1.903920553203156E-4
29	2.2847046638437872E-4	1.903920553203156E-4	0.0	0.37400615347122795	1.903920553203156E-4
30	2.2847046638437872E-4	2.2847046638437872E-4	0.0	0.5865217656197642	1.903920553203156E-4
31	3.046272885125049E-4	2.6654887744844184E-4	0.0	0.7886038931367472	1.903920553203156E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11745	0.0	23.186035	1
GTATTGG	1315	0.0	21.946768	1
ATTGGAC	1600	0.0	17.690624	3
TATTGGA	1730	0.0	17.002892	2
TTGGACC	2025	0.0	16.992594	4
TGGACCC	1955	0.0	16.749361	5
GGACCCT	2020	0.0	16.668318	6
CTAATAC	535	0.0	16.598131	3
TAATACG	105	9.352167E-6	15.857142	4
GACCCTC	2170	0.0	15.260368	7
TAATACT	600	0.0	15.108334	4
GTATCAA	18190	0.0	14.9403515	2
GTCGGGT	230	1.8189894E-12	14.47826	24
TTTTTAC	1745	0.0	14.418339	1
CTCTAAT	520	0.0	14.230769	1
ATACTGG	540	0.0	14.046295	6
GGTAAGA	330	0.0	14.015151	2
TAGCGAA	330	0.0	14.015151	10
GGCGTAT	80	0.0063014654	13.875	1
ACCGTTA	400	0.0	13.874999	8
>>END_MODULE