FastQCFastQC Report
Wed 25 May 2016
SRR2088497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245988
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT285261.2700869283362155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT248931.1083318343642086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT206450.9191945816273284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103530.46095526779306034No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG89470.39835475523466735No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA74200.33036685859407977No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG72350.32212994904692277No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA69880.31113256170558345No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA63450.28250373554978925No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG56930.25347419487548467No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA41010.18259224893454462No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA34570.15391889894336033No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34170.15213794552775883No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA32750.14581556090237346No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31460.14007198613705862No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG30720.13677722231819583No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC29370.13076650454054073No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28190.12551269196451628No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC27500.12244054732260368No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG26470.1178545922774298No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.11402554243388656No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG24910.11090887395658391No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24490.10903887287020232No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA24160.10756958630233109No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT22650.10084648715843539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71700.025.4665281
GTATTGG10450.018.5885181
TGGACCC14700.017.2414975
TTGGACC15600.017.0769234
ATTGGAC12250.016.9142863
GGACCCT15600.016.1282066
TAATACT4900.015.8571424
GTATCAA115250.015.8273322
GTATTAG4800.015.8020831
TTAACGG2700.015.75925935
ATTAGAC1659.749783E-1015.6969713
CGAACTA3900.015.65384724
CGGTATA855.366983E-415.23529326
TAACGGC2950.015.05084736
ATAATAC2109.094947E-1214.976193
TATTGGA13350.014.8277152
CCGAATT1001.09410175E-414.814
CGTTTAG908.2783506E-414.38888826
CGATAGA1051.6567411E-414.09523837
AGCGCGT800.006301087313.8750018
Produced by FastQC (version 0.11.5)