##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088497_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2245988
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.326433622975724	31.0	31.0	34.0	30.0	34.0
2	31.463396064449142	31.0	31.0	34.0	30.0	34.0
3	31.610644847612722	31.0	31.0	34.0	30.0	34.0
4	35.32247456353284	37.0	35.0	37.0	33.0	37.0
5	35.20336751576589	37.0	35.0	37.0	33.0	37.0
6	35.28178423036989	37.0	35.0	37.0	32.0	37.0
7	35.10940040641357	37.0	35.0	37.0	32.0	37.0
8	35.16650845863825	37.0	35.0	37.0	32.0	37.0
9	36.691938247221266	39.0	35.0	39.0	32.0	39.0
10	36.55193037540717	38.0	35.0	39.0	32.0	39.0
11	36.578745745747526	38.0	35.0	39.0	32.0	39.0
12	36.42210822141525	38.0	35.0	39.0	32.0	39.0
13	36.443220088442146	38.0	35.0	39.0	32.0	39.0
14	37.361458298085296	39.0	36.0	41.0	32.0	41.0
15	37.38509466657881	39.0	36.0	41.0	32.0	41.0
16	37.243540927199966	39.0	36.0	40.0	32.0	41.0
17	37.34249960373786	39.0	36.0	40.0	32.0	41.0
18	37.212142718482916	39.0	36.0	40.0	31.0	41.0
19	37.30674295677448	39.0	36.0	40.0	31.0	41.0
20	37.21474780809158	39.0	36.0	40.0	31.0	41.0
21	37.046811024814026	39.0	36.0	40.0	31.0	41.0
22	36.96018545067917	39.0	36.0	40.0	31.0	41.0
23	36.7471286578557	39.0	35.0	40.0	30.0	41.0
24	36.94897924654985	39.0	36.0	40.0	31.0	41.0
25	36.9162693656422	39.0	36.0	40.0	30.0	41.0
26	36.714504707950354	39.0	35.0	40.0	30.0	41.0
27	36.662963916102846	39.0	35.0	40.0	30.0	41.0
28	36.4620095922151	39.0	35.0	40.0	30.0	41.0
29	36.28742317412203	38.0	35.0	40.0	30.0	41.0
30	36.094218669022275	38.0	35.0	40.0	29.0	41.0
31	35.814033734819596	38.0	35.0	40.0	28.0	41.0
32	35.67239540015352	38.0	34.0	40.0	27.0	41.0
33	35.47456486855673	38.0	34.0	40.0	27.0	41.0
34	35.349436862529984	38.0	34.0	40.0	27.0	41.0
35	35.08210818579619	38.0	33.0	40.0	25.0	41.0
36	34.93473206446339	38.0	33.0	40.0	25.0	41.0
37	34.76713366233479	38.0	33.0	40.0	24.0	41.0
38	34.67494216353783	38.0	33.0	40.0	24.0	41.0
39	34.571043567463406	38.0	33.0	40.0	24.0	41.0
40	34.42156503062349	38.0	33.0	40.0	23.0	41.0
41	34.21284797603549	38.0	33.0	40.0	22.0	41.0
42	33.949302934833135	38.0	33.0	40.0	21.0	41.0
43	33.62614136852022	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	6.0
15	16.0
16	48.0
17	96.0
18	193.0
19	450.0
20	1157.0
21	2204.0
22	4082.0
23	7117.0
24	11397.0
25	16921.0
26	24342.0
27	33562.0
28	44389.0
29	57245.0
30	71747.0
31	87350.0
32	106342.0
33	128663.0
34	155552.0
35	188474.0
36	236414.0
37	312560.0
38	392692.0
39	362963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26985540439219	21.574736819609008	13.973004308126313	23.182403467872494
2	19.041998443446715	23.788150248353954	34.911005757822394	22.25884555037694
3	19.7338988454079	24.779339871806975	32.91509126495778	22.571670017827344
4	13.78012705321667	16.347059734958513	36.24511796144948	33.62769525037533
5	12.180296599981833	38.94949572304037	35.15780137738937	13.712406299588423
6	32.430093126054096	37.33897954931193	13.23836102419069	16.99256630044328
7	26.117993506643845	33.09087136707765	21.204432080670067	19.586703045608438
8	27.331624211705495	34.25574847238721	18.725077783140424	19.68754953276687
9	24.415268469822635	14.316772841172792	21.69374903160658	39.574209657397994
10	16.28962398730536	27.63318414880222	33.48855826478147	22.58863359911095
11	33.16722974477157	22.241392206904045	23.720696637738047	20.870681410586343
12	21.29147617885759	27.074320966986466	30.957556318199387	20.676646535956557
13	32.036190754358444	20.779229452695205	26.367772223181955	20.816807569764396
14	20.504250245326332	22.88765567759044	27.545828383766967	29.06226569331626
15	22.396335154061376	29.599312195790894	25.66273728978071	22.34161536036702
16	21.817881484674007	28.50504989340994	26.49760372717931	23.17946489473675
17	21.864720559504324	26.487007054356482	26.714790996211914	24.933481389927284
18	23.347097134980242	25.299957079022683	29.49726356507693	21.855682220920148
19	22.856399945146634	24.355250339716864	30.605417304099575	22.182932411036923
20	24.04389515883433	24.003734659312517	30.57589800123598	21.37647218061717
21	22.880843530775767	24.97515569985236	31.109338073044025	21.034662696327853
22	21.30242904236354	25.306591130495796	30.67937139468243	22.711608432458235
23	22.500654500380232	25.669193245912265	31.743001298315036	20.08715095539246
24	22.45225709131126	25.512825536022454	28.86680605595399	23.168111316712288
25	22.29718057264776	25.975962471749625	30.027052682382987	21.699804273219627
26	22.123938329145123	26.600186643917954	30.100027248587256	21.175847778349663
27	20.8056320870815	26.82004534307396	29.646284842127386	22.728037727717158
28	21.526962744235497	26.153657098791268	29.815252797432578	22.504127359540657
29	21.891969146763028	26.102855402611237	29.116718343998276	22.88845710662746
30	21.783687179094457	26.25036286925843	30.993086338840637	20.97286361280648
31	22.636407674484456	26.007040108851875	30.724384992261754	20.63216722440191
32	22.197803372057198	25.824447859917328	29.510398096516987	22.467350671508484
33	21.736046675227115	25.95721793705042	30.267214250476847	22.039521137245615
34	21.007458632904537	26.72160314302659	30.6763882977113	21.594549926357576
35	20.44022497003546	29.097484047109777	29.468367595908795	20.993923386945966
36	22.965438818016835	26.255661205669846	29.455010445291784	21.323889531021536
37	21.892369861281537	27.212344856695587	29.28435058424177	21.610934697781108
38	21.691433792166297	25.527117687182653	30.80679861156872	21.974649909082327
39	21.21133327515552	25.379832839712414	31.568556911256874	21.840276973875195
40	19.913597045042092	26.20601712920995	31.013611826955444	22.866773998792514
41	21.780436939110984	24.91500399824042	30.9775920441249	22.326967018523696
42	20.235415327241284	27.11229979857417	30.461249125106633	22.191035749077912
43	20.694812260795693	27.058381433916836	29.450691633258945	22.796114672028523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	264.0
2	370.0
3	866.5
4	1363.0
5	1363.0
6	2240.5
7	3118.0
8	3592.0
9	4066.0
10	6410.5
11	8755.0
12	8755.0
13	17029.0
14	25303.0
15	40735.5
16	56168.0
17	51654.0
18	47140.0
19	47140.0
20	49907.5
21	52675.0
22	41911.0
23	31147.0
24	34891.5
25	38636.0
26	38636.0
27	42313.0
28	45990.0
29	49852.5
30	53715.0
31	59141.5
32	64568.0
33	64568.0
34	72244.0
35	79920.0
36	87705.0
37	95490.0
38	101607.5
39	107725.0
40	107725.0
41	111304.0
42	114883.0
43	121877.5
44	128872.0
45	138583.0
46	148294.0
47	148294.0
48	198382.0
49	248470.0
50	231894.0
51	215318.0
52	197508.5
53	179699.0
54	179699.0
55	158511.5
56	137324.0
57	115576.0
58	93828.0
59	83222.5
60	72617.0
61	72617.0
62	64089.0
63	55561.0
64	48754.5
65	41948.0
66	36386.5
67	30825.0
68	30825.0
69	26546.5
70	22268.0
71	18906.0
72	15544.0
73	12949.0
74	10354.0
75	10354.0
76	8331.5
77	6309.0
78	4889.0
79	3469.0
80	2754.0
81	2039.0
82	2039.0
83	1534.0
84	1029.0
85	758.0
86	487.0
87	383.0
88	279.0
89	279.0
90	212.5
91	146.0
92	106.0
93	66.0
94	51.0
95	36.0
96	36.0
97	23.5
98	11.0
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2245988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.450837494021606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.04139756014554	40.07564981035156
2	10.787204854153407	10.668726285149221
3	3.2352717822784456	4.799606974633352
4	1.4079327710439857	2.7849381865361464
5	0.7492131339288656	1.8524608467151487
6	0.48529877388876347	1.4399058482172706
7	0.34698602014472035	1.201112450641171
8	0.25520294119378273	1.0095999338376083
9	0.19275501279060503	0.8578707132299638
>10	1.295208348392728	12.283821419611895
>50	0.11613756659046325	3.979857257121632
>100	0.07599482028432128	7.182927673659134
>500	0.005788655321655082	1.9388331323004282
>1k	0.004703282448844751	4.242326837814045
>5k	5.426864364051635E-4	1.9066295737681693
>10k+	3.6179095760344236E-4	3.775733056413333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28526	1.2700869283362155	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24893	1.1083318343642086	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20645	0.9191945816273284	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10353	0.46095526779306034	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8947	0.39835475523466735	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7420	0.33036685859407977	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7235	0.32212994904692277	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6988	0.31113256170558345	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6345	0.28250373554978925	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5693	0.25347419487548467	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4101	0.18259224893454462	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3457	0.15391889894336033	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3417	0.15213794552775883	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3275	0.14581556090237346	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3146	0.14007198613705862	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3072	0.13677722231819583	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2937	0.13076650454054073	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2819	0.12551269196451628	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2750	0.12244054732260368	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2647	0.1178545922774298	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2561	0.11402554243388656	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2491	0.11090887395658391	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2449	0.10903887287020232	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2416	0.10756958630233109	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2265	0.10084648715843539	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.4523835390037704E-5	0.0	0.0	0.0	0.0
6	1.3357150617011312E-4	0.0	0.0	0.0	0.0
7	1.3357150617011312E-4	0.0	4.4523835390037704E-5	0.0	0.0
8	2.2261917695018851E-4	0.0	4.4523835390037704E-5	0.0	0.0
9	2.2261917695018851E-4	0.0	4.4523835390037704E-5	8.904767078007541E-5	0.0
10	2.6714301234022624E-4	0.0	4.4523835390037704E-5	2.6714301234022624E-4	0.0
11	3.116668477302639E-4	0.0	4.4523835390037704E-5	3.5619068312030163E-4	0.0
12	3.5619068312030163E-4	0.0	4.4523835390037704E-5	7.56905201630641E-4	0.0
13	4.007145185103393E-4	0.0	4.4523835390037704E-5	8.904767078007541E-4	0.0
14	4.007145185103393E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.0012911912263110935	0.0
15	4.007145185103393E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.0014247627324812065	0.0
16	4.4523835390037703E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.0019145249217716212	0.0
17	4.4523835390037703E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.002537858617232149	0.0
18	4.4523835390037703E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.003116668477302639	0.0
19	4.4523835390037703E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.004007145185103393	0.0
20	4.4523835390037703E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.007569052016306409	0.0
21	4.4523835390037703E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.013490722123181424	0.0
22	4.4523835390037703E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.022039298518068663	0.0
23	4.4523835390037703E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.027560254106433337	0.0
24	4.897621892904148E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.03553002064125009	0.0
25	4.897621892904148E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.04105097622961476	0.0
26	4.897621892904148E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.048441932904361024	0.0
27	4.897621892904148E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.09501386472234045	0.0
28	4.897621892904148E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.19243201655574296	0.0
29	4.897621892904148E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.315273278396857	0.0
30	5.788098600704901E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.4890052840887841	0.0
31	5.788098600704901E-4	4.4523835390037704E-5	4.4523835390037704E-5	0.6716420568587187	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7170	0.0	25.466528	1
GTATTGG	1045	0.0	18.588518	1
TGGACCC	1470	0.0	17.241497	5
TTGGACC	1560	0.0	17.076923	4
ATTGGAC	1225	0.0	16.914286	3
GGACCCT	1560	0.0	16.128206	6
TAATACT	490	0.0	15.857142	4
GTATCAA	11525	0.0	15.827332	2
GTATTAG	480	0.0	15.802083	1
TTAACGG	270	0.0	15.759259	35
ATTAGAC	165	9.749783E-10	15.696971	3
CGAACTA	390	0.0	15.653847	24
CGGTATA	85	5.366983E-4	15.235293	26
TAACGGC	295	0.0	15.050847	36
ATAATAC	210	9.094947E-12	14.97619	3
TATTGGA	1335	0.0	14.827715	2
CCGAATT	100	1.09410175E-4	14.8	14
CGTTTAG	90	8.2783506E-4	14.388888	26
CGATAGA	105	1.6567411E-4	14.095238	37
AGCGCGT	80	0.0063010873	13.875001	8
>>END_MODULE