FastQCFastQC Report
Wed 25 May 2016
SRR2088496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402090
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT345841.4397462209992131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT290211.2081562306158387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT239930.9988385114629343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126970.5285813603986529No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG87910.36597296520946343No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG79560.33121156992452405No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA74430.30985516779138167No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA69260.2883322440041797No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA67590.2813799649471918No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG63420.2640200825114796No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39270.16348263387300227No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38980.16227535188107023No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC37690.15690502853764846No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.15207590056992037No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33490.13942025486139153No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31790.132343084563859No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29170.12143591622295585No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA29050.12093635126077706No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG28520.11872993934448751No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC27450.11427548509839347No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26550.11052874788205272No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA25870.10769787976303968No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG25390.10569961991432461No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG25280.10524168536566074No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT24420.10166146980337956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102600.021.6374261
GTATTGG9600.019.8489571
TTGGACC14800.017.8750024
ATTGGAC10800.017.4722213
TGGACCC14600.017.3595895
GGACCCT15250.016.9836066
CTAATAC6150.016.2439023
TAATACT6000.015.7254
TCTAATA4900.015.4795912
TATACTG2550.015.2352945
TCTATAC1954.1836756E-1115.1794863
TATACGG1752.242814E-914.82
GACCCTC16900.014.668647
TTCGTTA2301.8189894E-1214.4782630
AGTTCCG2301.8189894E-1214.4782632
CTCTAAT4350.014.459771
TGCGATA2750.014.12727212
TATTGGA12850.014.1089492
GTATCAA157750.014.0846272
TATACCG1356.573635E-613.7037035
Produced by FastQC (version 0.11.5)