##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2402090
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26506958523619	31.0	31.0	33.0	30.0	34.0
2	31.39734647744256	31.0	31.0	34.0	30.0	34.0
3	31.54180775907647	31.0	31.0	34.0	30.0	34.0
4	35.260245452918085	37.0	35.0	37.0	33.0	37.0
5	35.13096345265998	35.0	35.0	37.0	32.0	37.0
6	35.21945805527686	37.0	35.0	37.0	32.0	37.0
7	35.04098763993023	37.0	35.0	37.0	32.0	37.0
8	35.10157487854327	37.0	35.0	37.0	32.0	37.0
9	36.61574836912855	39.0	35.0	39.0	32.0	39.0
10	36.477875933041645	38.0	35.0	39.0	32.0	39.0
11	36.49990133591997	38.0	35.0	39.0	32.0	39.0
12	36.350304110170725	38.0	35.0	39.0	32.0	39.0
13	36.38678692305451	38.0	35.0	39.0	32.0	39.0
14	37.262723711434624	39.0	36.0	40.0	32.0	41.0
15	37.29890262229975	39.0	36.0	40.0	32.0	41.0
16	37.14450041422261	39.0	36.0	40.0	31.0	41.0
17	37.25476980462847	39.0	36.0	40.0	32.0	41.0
18	37.13156709365594	39.0	36.0	40.0	31.0	41.0
19	37.2362426053978	39.0	36.0	40.0	31.0	41.0
20	37.151014325025294	39.0	36.0	40.0	31.0	41.0
21	36.99580157279702	39.0	36.0	40.0	31.0	41.0
22	36.90969530700348	39.0	36.0	40.0	31.0	41.0
23	36.7097298602467	39.0	35.0	40.0	30.0	41.0
24	36.903148091869994	39.0	36.0	40.0	30.0	41.0
25	36.865624518648346	39.0	36.0	40.0	30.0	41.0
26	36.64985158757582	39.0	35.0	40.0	30.0	41.0
27	36.59010944635713	39.0	35.0	40.0	30.0	41.0
28	36.3917376118297	38.0	35.0	40.0	30.0	41.0
29	36.21919453475931	38.0	35.0	40.0	30.0	41.0
30	36.028359053990485	38.0	35.0	40.0	29.0	41.0
31	35.741254490880856	38.0	34.0	40.0	28.0	41.0
32	35.6044332227352	38.0	34.0	40.0	27.0	41.0
33	35.39617624651866	38.0	34.0	40.0	27.0	41.0
34	35.286104600577	38.0	34.0	40.0	26.0	41.0
35	35.007293232143674	38.0	33.0	40.0	25.0	41.0
36	34.83433010420092	38.0	33.0	40.0	25.0	41.0
37	34.668288448809164	38.0	33.0	40.0	24.0	41.0
38	34.55824094850734	38.0	33.0	40.0	24.0	41.0
39	34.44118455178615	38.0	33.0	40.0	23.0	41.0
40	34.28449641770292	38.0	33.0	40.0	23.0	41.0
41	34.046177287278994	37.0	33.0	40.0	21.0	41.0
42	33.785400213980324	37.0	33.0	40.0	20.0	41.0
43	33.439475207007234	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	7.0
15	8.0
16	50.0
17	128.0
18	233.0
19	554.0
20	1231.0
21	2558.0
22	4508.0
23	7807.0
24	12307.0
25	18735.0
26	26717.0
27	37055.0
28	49437.0
29	63512.0
30	79149.0
31	96311.0
32	116893.0
33	140760.0
34	168965.0
35	203664.0
36	254808.0
37	334972.0
38	412927.0
39	368786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16610951296579	21.684075117918148	14.437510667793463	22.712304701322598
2	19.11518719115437	24.3931742774001	34.53088768530738	21.960750846138154
3	20.07243691951592	24.830918075509246	33.013542373516394	22.083102631458438
4	13.977577859280876	16.544259374128366	35.908562959755876	33.56959980683488
5	12.203039852794857	39.21876365997943	34.65244849277088	13.925747994454829
6	32.24541961375302	37.349058528198356	13.060043545412537	17.34547831263608
7	25.785212044511237	33.43280226802493	21.060076849743346	19.721908837720484
8	27.714115624310498	34.13544038732937	18.58439941883943	19.56604456952071
9	24.530846054893864	14.342135390430833	21.881320017151733	39.24569853752357
10	16.63272400284752	27.91902051963082	33.00733944190268	22.440916035618983
11	32.926285026789174	22.49045622770171	23.53371439038504	21.04954435512408
12	21.331340624206423	27.596801119025514	30.75575852694945	20.316099729818614
13	32.35952857719735	20.870034012047842	26.095983081399947	20.67445432935485
14	20.404397836883714	23.333721883859475	28.04133067453759	28.220549604719224
15	22.674212872956467	29.709960909041712	25.753406408585853	21.862419809415968
16	21.564637461543906	28.489690228093036	26.748290030764878	23.197382279598184
17	21.763589207731602	26.69546103601447	26.989871320391824	24.551078435862102
18	23.16973968502429	25.30816913604403	29.677281034432518	21.844810144499167
19	22.744734793450704	24.53226148895337	31.125228446894166	21.597775270701764
20	23.81355403003218	24.10713170613924	30.87157433734789	21.20773992648069
21	22.53117077211928	25.177949202569426	31.542073777418832	20.74880624789246
22	20.900340953086687	25.674183731667004	31.06265793538127	22.362817379865035
23	22.19429746595673	25.764604989821365	31.883692950722082	20.157404593499827
24	22.143425100641526	25.85298635771349	29.30169144370111	22.701897097943874
25	21.911169023641914	26.105308294027285	30.415638048532735	21.56788463379807
26	21.788192782118905	26.94103884533885	30.477126169294237	20.793642203248005
27	20.523918754084985	26.900865496296976	30.13717221253159	22.438043537086454
28	21.047004899899672	26.58085250760796	30.18800294743328	22.184139645059094
29	21.489119891427883	26.315500251863998	29.565128700423383	22.630251156284736
30	21.294247925764648	26.600210649892386	31.521883026864106	20.583658397478864
31	22.27002318814033	26.3884367363421	31.017364045477063	20.324176030040505
32	21.6342018825273	26.175039236664738	29.93784579262226	22.252913088185704
33	21.25603120615797	26.29472667552007	30.671415309168264	21.777826809153694
34	20.49302898725693	26.99536653497579	31.170106032663224	21.341498445104055
35	19.984430225345427	29.1999467130707	30.01432086224912	20.801302199334746
36	22.53516729181671	26.659200945842993	29.821821830156235	20.983809932184055
37	21.427465249012318	27.50883605526854	29.67877972931905	21.384918966400093
38	21.135469528618827	25.899487529609633	31.30906835297595	21.65597458879559
39	20.654804774175823	25.583138017309924	31.959335412078648	21.802721796435602
40	19.497146235153554	26.40225803362905	31.470094792451576	22.630500938765824
41	21.09317302848769	25.21770624747616	31.592030273636706	22.097090450399442
42	19.73377350557223	27.34618602966583	30.993051883984364	21.926988580777575
43	20.147371663842737	27.202602733452952	30.085717021427172	22.56430858127714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	348.5
2	501.0
3	1151.0
4	1801.0
5	1801.0
6	2934.5
7	4068.0
8	4750.0
9	5432.0
10	8323.5
11	11215.0
12	11215.0
13	21428.5
14	31642.0
15	50187.0
16	68732.0
17	62621.0
18	56510.0
19	56510.0
20	59092.5
21	61675.0
22	48088.0
23	34501.0
24	37803.5
25	41106.0
26	41106.0
27	45157.0
28	49208.0
29	53301.0
30	57394.0
31	62501.0
32	67608.0
33	67608.0
34	75553.0
35	83498.0
36	91244.0
37	98990.0
38	105889.5
39	112789.0
40	112789.0
41	116207.0
42	119625.0
43	126999.5
44	134374.0
45	145738.5
46	157103.0
47	157103.0
48	211290.0
49	265477.0
50	247413.0
51	229349.0
52	209999.0
53	190649.0
54	190649.0
55	170134.0
56	149619.0
57	125527.5
58	101436.0
59	89719.0
60	78002.0
61	78002.0
62	68886.5
63	59771.0
64	51415.5
65	43060.0
66	36629.0
67	30198.0
68	30198.0
69	25764.0
70	21330.0
71	17900.5
72	14471.0
73	11739.0
74	9007.0
75	9007.0
76	7177.5
77	5348.0
78	4135.0
79	2922.0
80	2295.5
81	1669.0
82	1669.0
83	1267.5
84	866.0
85	642.5
86	419.0
87	334.5
88	250.0
89	250.0
90	212.0
91	174.0
92	118.0
93	62.0
94	45.0
95	28.0
96	28.0
97	18.5
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2402090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.10938081402937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27806094362427	39.42405865892303
2	11.291191186927977	11.090068156857164
3	3.489583544545652	5.141138615143886
4	1.505902550236644	2.958157672335574
5	0.8027029383615993	1.9710122140270059
6	0.4826447257479104	1.4221430180782137
7	0.3422825564210695	1.1766499086497255
8	0.25034963597969334	0.983561248798431
9	0.18298665657937105	0.8087725261658112
>10	1.1640030331916662	11.014568166211514
>50	0.11938280106310621	4.044908525652454
>100	0.07975207215939131	7.563572307953119
>500	0.005195409655348927	1.7483593577418959
>1k	0.005110239005261228	4.608558152026728
>5k	5.110239005261228E-4	1.8494546823271114
>10k+	3.406826003507485E-4	4.195016789108209
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34584	1.4397462209992131	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29021	1.2081562306158387	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23993	0.9988385114629343	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12697	0.5285813603986529	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8791	0.36597296520946343	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7956	0.33121156992452405	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7443	0.30985516779138167	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6926	0.2883322440041797	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6759	0.2813799649471918	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6342	0.2640200825114796	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3927	0.16348263387300227	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3898	0.16227535188107023	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3769	0.15690502853764846	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3653	0.15207590056992037	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3349	0.13942025486139153	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3179	0.132343084563859	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2917	0.12143591622295585	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2905	0.12093635126077706	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2852	0.11872993934448751	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2745	0.11427548509839347	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2655	0.11052874788205272	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2587	0.10769787976303968	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2539	0.10569961991432461	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2528	0.10524168536566074	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2442	0.10166146980337956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.163041351489745E-5	0.0
3	0.0	0.0	0.0	4.163041351489745E-5	0.0
4	0.0	0.0	0.0	4.163041351489745E-5	0.0
5	0.0	0.0	0.0	4.163041351489745E-5	0.0
6	0.0	0.0	0.0	4.163041351489745E-5	0.0
7	0.0	0.0	0.0	4.163041351489745E-5	0.0
8	4.163041351489745E-5	0.0	0.0	4.163041351489745E-5	0.0
9	8.32608270297949E-5	0.0	0.0	1.665216540595898E-4	0.0
10	1.2489124054469234E-4	0.0	0.0	2.914128946042821E-4	0.0
11	1.2489124054469234E-4	0.0	0.0	3.74673721634077E-4	0.0
12	1.2489124054469234E-4	0.0	0.0	5.411953756936668E-4	0.0
13	1.2489124054469234E-4	0.0	0.0	5.828257892085642E-4	0.0
14	1.2489124054469234E-4	0.0	0.0	8.326082702979488E-4	0.0
15	1.2489124054469234E-4	0.0	0.0	0.0011656515784171284	0.0
16	1.665216540595898E-4	0.0	0.0	0.0019149990216852824	0.0
17	1.665216540595898E-4	0.0	0.0	0.002914128946042821	0.0
18	2.081520675744872E-4	0.0	0.0	0.0035802155622811803	0.0
19	2.081520675744872E-4	0.0	0.0	0.0045377150731238216	0.0
20	2.081520675744872E-4	0.0	0.0	0.007202061538077258	0.0
21	2.497824810893847E-4	0.0	0.0	0.01253075446798413	0.0
22	2.497824810893847E-4	0.0	0.0	0.019816076833091185	0.0
23	2.914128946042821E-4	0.0	0.0	0.023812596530521337	0.0
24	2.914128946042821E-4	0.0	0.0	0.03026531062533044	0.0
25	2.914128946042821E-4	0.0	0.0	0.03555237314172242	0.0
26	2.914128946042821E-4	0.0	4.163041351489745E-5	0.04379519501767211	0.0
27	2.914128946042821E-4	0.0	4.163041351489745E-5	0.09999625326278366	0.0
28	3.330433081191796E-4	0.0	4.163041351489745E-5	0.21244000016652165	0.0
29	3.330433081191796E-4	4.163041351489745E-5	4.163041351489745E-5	0.35843786036326697	0.0
30	3.330433081191796E-4	4.163041351489745E-5	4.163041351489745E-5	0.559387866399677	0.0
31	4.163041351489744E-4	4.163041351489745E-5	4.163041351489745E-5	0.7786136239691269	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10260	0.0	21.637426	1
GTATTGG	960	0.0	19.848957	1
TTGGACC	1480	0.0	17.875002	4
ATTGGAC	1080	0.0	17.472221	3
TGGACCC	1460	0.0	17.359589	5
GGACCCT	1525	0.0	16.983606	6
CTAATAC	615	0.0	16.243902	3
TAATACT	600	0.0	15.725	4
TCTAATA	490	0.0	15.479591	2
TATACTG	255	0.0	15.235294	5
TCTATAC	195	4.1836756E-11	15.179486	3
TATACGG	175	2.242814E-9	14.8	2
GACCCTC	1690	0.0	14.66864	7
TTCGTTA	230	1.8189894E-12	14.47826	30
AGTTCCG	230	1.8189894E-12	14.47826	32
CTCTAAT	435	0.0	14.45977	1
TGCGATA	275	0.0	14.127272	12
TATTGGA	1285	0.0	14.108949	2
GTATCAA	15775	0.0	14.084627	2
TATACCG	135	6.573635E-6	13.703703	5
>>END_MODULE