FastQCFastQC Report
Wed 25 May 2016
SRR2088495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038791
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT183081.7624334442635718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162131.5607566873413419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96030.9244400461690561No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64910.6248610163160828No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG50850.48951136465371764No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG40370.3886248533150557No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA35210.33895172368647786No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA29690.2858130268745109No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27200.2618428538560692No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG22970.22112243945124668No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC19640.18906594300489704No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.18627423610716687No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.16856133716984456No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13590.13082516117294046No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG13460.1295737063567166No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC13350.12851478305068104No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA13280.12784092276502204No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG12700.12225750896956172No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG11870.11426745129674785No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC11800.11359359101108886No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.11214960468467669No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA10740.10338942097110969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10710.10310062370582726No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA10480.10088651133866196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43900.025.1161751
CGTAGAC400.00193106323.1253
CGTAATA450.003825563520.5555572
TCACGTA2500.017.7625
CCGGTCT2550.017.4117649
CTACCCT650.001579795817.0769234
GTATCAA64650.017.0262952
TCCGGTC2650.016.7547158
CCCATAG2000.016.651
CTAGTCC904.445293E-516.4444453
GTATTGG3300.016.2575761
CGGTCTG2850.016.2280710
ATACTCG803.3821742E-416.187526
CACGTAG2750.016.14545426
TACTCGC700.002592268415.85714327
CGAACTA1751.3096724E-1015.85714224
GTATACT957.058631E-515.5789484
GTAAGAT1455.3429176E-815.3103453
ACGGGTA855.363534E-415.2352945
GATACCT2850.014.92982436
Produced by FastQC (version 0.11.5)