##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038791
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.662147631236696	31.0	30.0	31.0	28.0	34.0
2	30.808461952404286	31.0	31.0	33.0	27.0	34.0
3	30.8266773585832	31.0	30.0	34.0	27.0	34.0
4	34.59579645953806	35.0	35.0	37.0	32.0	37.0
5	34.378104931598365	35.0	35.0	37.0	30.0	37.0
6	34.624119770001855	35.0	35.0	37.0	31.0	37.0
7	34.448480974517494	35.0	35.0	37.0	30.0	37.0
8	34.56734126498978	35.0	35.0	37.0	30.0	37.0
9	35.95317826203731	37.0	35.0	39.0	30.0	39.0
10	35.70386054557654	37.0	35.0	39.0	30.0	39.0
11	35.83238399254518	37.0	35.0	39.0	30.0	39.0
12	35.58050271902625	37.0	35.0	39.0	30.0	39.0
13	35.69707958578771	37.0	35.0	39.0	30.0	39.0
14	36.36761966555351	38.0	35.0	40.0	30.0	41.0
15	36.453433847617084	38.0	35.0	40.0	30.0	41.0
16	36.22251829290011	38.0	34.0	40.0	30.0	41.0
17	36.38841499396895	38.0	35.0	40.0	30.0	41.0
18	36.26473563979665	38.0	35.0	40.0	30.0	41.0
19	36.39101320669894	38.0	35.0	40.0	30.0	41.0
20	36.29153602601486	38.0	34.0	40.0	30.0	41.0
21	36.11902009162574	38.0	34.0	40.0	29.0	41.0
22	36.00883623366009	38.0	34.0	40.0	29.0	41.0
23	35.78113788047836	38.0	34.0	40.0	27.0	41.0
24	35.973722336831955	38.0	34.0	40.0	29.0	41.0
25	35.91081940448078	38.0	34.0	40.0	29.0	41.0
26	35.62848445933783	38.0	34.0	40.0	27.0	41.0
27	35.54599914708541	38.0	34.0	40.0	27.0	41.0
28	35.298497965423266	38.0	34.0	40.0	27.0	41.0
29	35.12912029465022	38.0	33.0	40.0	26.0	41.0
30	34.906250631744015	37.0	33.0	40.0	25.0	41.0
31	34.55616096019315	37.0	33.0	40.0	25.0	41.0
32	34.39261218089106	37.0	33.0	40.0	25.0	41.0
33	34.155954373882714	37.0	33.0	40.0	24.0	41.0
34	34.00229208762879	36.0	33.0	39.0	24.0	40.0
35	33.680142588836446	36.0	32.0	39.0	23.0	40.0
36	33.530980726633175	36.0	32.0	39.0	22.0	40.0
37	33.33164419021728	36.0	31.0	39.0	21.0	40.0
38	33.17688639967039	36.0	31.0	39.0	20.0	40.0
39	33.02166075755373	36.0	31.0	39.0	19.0	40.0
40	32.835234421553515	36.0	31.0	39.0	18.0	40.0
41	32.554592791042666	36.0	30.0	39.0	16.0	40.0
42	32.24618041550225	35.0	30.0	39.0	15.0	40.0
43	31.873094780374494	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	2.0
14	11.0
15	20.0
16	55.0
17	118.0
18	256.0
19	577.0
20	1186.0
21	2180.0
22	3698.0
23	5879.0
24	8840.0
25	12755.0
26	17381.0
27	22781.0
28	28996.0
29	36448.0
30	44047.0
31	52621.0
32	62128.0
33	73195.0
34	85026.0
35	101934.0
36	125448.0
37	148286.0
38	146609.0
39	58309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.71356220837493	25.04873453851641	16.00139007750356	20.236313175605105
2	22.61446238945081	25.992620267214484	31.23765993351887	20.15525740981583
3	23.20774823809602	25.106686523083084	32.51212226521023	19.17344297361067
4	17.257369384216844	18.852974274902266	33.27984166208602	30.609814678794866
5	11.759728376545427	41.379449764197034	33.94696334488843	12.913858514369108
6	32.11887665565066	38.02843882936991	12.595507662272777	17.257176852706657
7	24.87978813832619	34.338668702366505	20.819298588455233	19.96224457085208
8	28.121633706876555	35.101767342997775	18.07533950525178	18.701259444873898
9	24.36765432122535	13.973937009465812	21.89381694681606	39.76459172249278
10	16.88944166824703	29.071006583614988	32.48564918255934	21.55390256557864
11	33.770508215800874	22.41037898865123	22.989224974032314	20.829887821515587
12	20.21272806560704	28.93228763052433	31.027993118923824	19.826991184944806
13	32.97670079929457	20.70560873168905	26.53093836970093	19.786752099315454
14	19.793779499437328	23.678102717486	28.4742551677864	28.053862615290274
15	22.6278433294089	30.29242648424948	26.16869033328167	20.91103985305995
16	20.963600955341356	28.586115975205793	27.735993091969412	22.71428997748344
17	21.525311636315678	27.257359757641336	27.80135753967834	23.415971066364648
18	22.173083902344167	25.56481525157611	30.44914713354274	21.812953712536977
19	22.439354980934567	24.54083641463971	32.154398719280394	20.86540988514533
20	23.06652637537291	24.17791451793479	32.51606916116909	20.23948994552321
21	22.00413750215395	25.317315995229066	33.00028590929263	19.678260593324353
22	20.481309522319695	25.57145758867761	32.39795107966857	21.54928180933412
23	21.728047316543943	25.82203734918766	33.33846750693835	19.111447827330043
24	21.663356729120682	26.106983984266325	30.360293841590853	21.869365445022147
25	21.52396391574436	26.536040454720922	31.459167435990494	20.480828193544227
26	21.324886334209673	27.078209187411133	31.65670476544367	19.94019971293552
27	20.464366749423128	26.7582218174782	31.34008669693904	21.43732473615963
28	20.704742339893205	26.837448533920682	31.660170332627064	20.797638793559052
29	21.089324031494307	26.03151163227252	31.085463774715027	21.79370056151815
30	21.237958357359663	26.305676502780635	32.68713340797138	19.769231731888322
31	21.70706138193342	26.605640595653984	32.016834955250864	19.670463067161727
32	21.442523086934713	26.180145958137874	31.094705287204068	21.282625667723345
33	21.102993768717674	26.667635741934614	31.385427867588376	20.843942621759336
34	20.26856220356164	27.232041864051578	32.20089507899087	20.298500853395918
35	19.703771018424305	29.228304827438823	31.125029000058724	19.942895154078155
36	22.533214092151358	26.439678433871684	30.915362185463678	20.11174528851328
37	21.147853610591543	27.545290631127916	30.75844900466023	20.548406753620316
38	21.13360627883761	25.646448611895945	32.33133517714343	20.888609932123018
39	20.405355841550417	25.83715107273744	32.94887999607236	20.80861308963978
40	19.32958602837337	26.749076570744258	31.828731669796905	22.092605731085463
41	21.148720002387392	25.572323980473456	31.907188260198634	21.37176775694052
42	20.07218006316959	27.63809081903867	30.860201907794732	21.42952720999701
43	20.618295691818663	27.12691965948877	30.094889154796295	22.15989549389627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	133.0
2	166.0
3	284.0
4	402.0
5	402.0
6	845.0
7	1288.0
8	1587.5
9	1887.0
10	3083.0
11	4279.0
12	4279.0
13	9638.5
14	14998.0
15	25145.0
16	35292.0
17	30343.0
18	25394.0
19	25394.0
20	28835.0
21	32276.0
22	24528.0
23	16780.0
24	17747.5
25	18715.0
26	18715.0
27	20114.5
28	21514.0
29	23367.5
30	25221.0
31	27971.0
32	30721.0
33	30721.0
34	34781.0
35	38841.0
36	42551.5
37	46262.0
38	49197.0
39	52132.0
40	52132.0
41	53744.0
42	55356.0
43	58500.5
44	61645.0
45	65874.0
46	70103.0
47	70103.0
48	93016.5
49	115930.0
50	105769.0
51	95608.0
52	85912.0
53	76216.0
54	76216.0
55	66508.0
56	56800.0
57	47967.5
58	39135.0
59	34371.5
60	29608.0
61	29608.0
62	26162.0
63	22716.0
64	19535.5
65	16355.0
66	13928.5
67	11502.0
68	11502.0
69	9765.0
70	8028.0
71	6672.0
72	5316.0
73	4408.5
74	3501.0
75	3501.0
76	2756.5
77	2012.0
78	1631.5
79	1251.0
80	966.5
81	682.0
82	682.0
83	525.0
84	368.0
85	275.5
86	183.0
87	145.0
88	107.0
89	107.0
90	87.0
91	67.0
92	46.0
93	25.0
94	16.0
95	7.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1038791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.0845334197647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.79182924880408	47.8088741881826
2	7.575713574606628	8.346092951579678
3	2.062450806516915	3.4082742113460505
4	0.9284981312956245	2.0458354537417165
5	0.48769749779067584	1.3432294557893052
6	0.3421143379884839	1.1307125210584392
7	0.2567599215802152	0.9900450336799069
8	0.19123274107262053	0.8427173053254381
9	0.1487211119914176	0.7373009757344231
>10	1.0282459504969266	10.76657560054848
>50	0.10894834802110016	4.19119014787656
>100	0.06670085309820654	7.100193530950776
>500	0.006863676075312579	2.539065637246435
>1k	0.003343842190536897	3.3501008448064242
>5k	5.279750827163523E-4	2.053181288658022
>10k+	3.5198338847756814E-4	3.3466108534757817
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18308	1.7624334442635718	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16213	1.5607566873413419	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9603	0.9244400461690561	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6491	0.6248610163160828	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5085	0.48951136465371764	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4037	0.3886248533150557	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3521	0.33895172368647786	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2969	0.2858130268745109	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2720	0.2618428538560692	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2297	0.22112243945124668	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1964	0.18906594300489704	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1935	0.18627423610716687	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1751	0.16856133716984456	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1359	0.13082516117294046	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1346	0.1295737063567166	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1335	0.12851478305068104	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1328	0.12784092276502204	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1270	0.12225750896956172	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1187	0.11426745129674785	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	1180	0.11359359101108886	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1165	0.11214960468467669	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1074	0.10338942097110969	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1071	0.10310062370582726	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1048	0.10088651133866196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.62657550941431E-5	0.0
11	9.62657550941431E-5	0.0	0.0	9.62657550941431E-5	0.0
12	9.62657550941431E-5	0.0	0.0	9.62657550941431E-5	0.0
13	9.62657550941431E-5	0.0	0.0	1.925315101882862E-4	0.0
14	9.62657550941431E-5	0.0	0.0	2.887972652824293E-4	0.0
15	9.62657550941431E-5	0.0	0.0	3.850630203765724E-4	0.0
16	9.62657550941431E-5	0.0	0.0	3.850630203765724E-4	0.0
17	9.62657550941431E-5	0.0	0.0	6.738602856590016E-4	0.0
18	9.62657550941431E-5	0.0	0.0	0.0014439863264121464	0.0
19	9.62657550941431E-5	0.0	0.0	0.0024066438773535774	0.0
20	9.62657550941431E-5	0.0	0.0	0.004717021999613011	0.0
21	9.62657550941431E-5	0.0	0.0	0.007893791917719734	0.0
22	9.62657550941431E-5	0.0	0.0	0.013766002978462463	0.0
23	9.62657550941431E-5	0.0	0.0	0.01732783591694576	0.0
24	9.62657550941431E-5	0.0	0.0	0.021852326406370484	0.0
25	9.62657550941431E-5	0.0	0.0	0.02493283056938306	0.0
26	9.62657550941431E-5	0.0	0.0	0.03138263616069065	0.0
27	9.62657550941431E-5	0.0	0.0	0.06902254640250059	0.0
28	9.62657550941431E-5	0.0	0.0	0.14324344358008492	0.0
29	9.62657550941431E-5	0.0	0.0	0.23787268083762758	0.0
30	1.925315101882862E-4	0.0	0.0	0.3815974531931832	0.0
31	1.925315101882862E-4	0.0	0.0	0.5214715953449731	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4390	0.0	25.116175	1
CGTAGAC	40	0.001931063	23.125	3
CGTAATA	45	0.0038255635	20.555557	2
TCACGTA	250	0.0	17.76	25
CCGGTCT	255	0.0	17.411764	9
CTACCCT	65	0.0015797958	17.076923	4
GTATCAA	6465	0.0	17.026295	2
TCCGGTC	265	0.0	16.754715	8
CCCATAG	200	0.0	16.65	1
CTAGTCC	90	4.445293E-5	16.444445	3
GTATTGG	330	0.0	16.257576	1
CGGTCTG	285	0.0	16.22807	10
ATACTCG	80	3.3821742E-4	16.1875	26
CACGTAG	275	0.0	16.145454	26
TACTCGC	70	0.0025922684	15.857143	27
CGAACTA	175	1.3096724E-10	15.857142	24
GTATACT	95	7.058631E-5	15.578948	4
GTAAGAT	145	5.3429176E-8	15.310345	3
ACGGGTA	85	5.363534E-4	15.235294	5
GATACCT	285	0.0	14.929824	36
>>END_MODULE