FastQCFastQC Report
Wed 25 May 2016
SRR2088494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408671
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT291321.2094636419834837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT244161.0136710243947804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT193140.8018529720331253No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112780.4682250087288799No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59980.24901698903669287No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58440.2426234217956707No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA51120.2122332190656175No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48660.2020201181481406No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG47030.19525290087355227No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45950.1907691004707575No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31350.1301547616922361No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30970.12857712821717868No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA30610.12708252808291376No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28760.11940194405960797No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.10748665965588493No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25850.10732059297429994No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25690.10665632624795997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84900.021.9210831
GTATTGG8250.021.3030321
TCTAACG851.246468E-619.5882362
ATTGGAC10600.017.1037733
GTATACT1901.8189894E-1216.5526314
CTCTAAT4850.016.4020611
CTAATAC6000.016.0333333
TAGGTCG1403.4780896E-815.85714321
CGGTATA1059.351525E-615.85714228
TATACCG1059.351525E-615.8571425
CTGTGCG2003.6379788E-1215.7249999
TTGGACC14100.015.4822694
TGGACCC13000.015.369235
CGGGTAA2800.015.19642926
GGACCCT13150.015.1939166
AATACTG7100.015.1126765
TCGGGTA2700.015.07407525
CATTGCG1508.112329E-814.829
GTCGTCA1651.6474587E-814.57575724
GTTATAC1152.211722E-514.4782613
Produced by FastQC (version 0.11.5)