##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088494_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2408671
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.320046199750816	31.0	31.0	34.0	30.0	34.0
2	31.45614739414391	31.0	31.0	34.0	30.0	34.0
3	31.59993207872723	31.0	31.0	34.0	30.0	34.0
4	35.32318236903255	37.0	35.0	37.0	33.0	37.0
5	35.18697323129643	37.0	35.0	37.0	33.0	37.0
6	35.265120890316695	37.0	35.0	37.0	32.0	37.0
7	35.095943779785614	37.0	35.0	37.0	32.0	37.0
8	35.14928398274401	37.0	35.0	37.0	32.0	37.0
9	36.67898729216236	39.0	35.0	39.0	32.0	39.0
10	36.52879658533689	38.0	35.0	39.0	32.0	39.0
11	36.56606028801775	38.0	35.0	39.0	32.0	39.0
12	36.41876702961924	38.0	35.0	39.0	32.0	39.0
13	36.44454597576838	38.0	35.0	39.0	32.0	39.0
14	37.373626369064105	39.0	36.0	41.0	32.0	41.0
15	37.39740421170014	39.0	36.0	41.0	32.0	41.0
16	37.265151197486084	39.0	36.0	40.0	32.0	41.0
17	37.34930590354598	39.0	36.0	40.0	32.0	41.0
18	37.22434861382065	39.0	36.0	40.0	31.0	41.0
19	37.316780083290745	39.0	36.0	40.0	31.0	41.0
20	37.22713521273764	39.0	36.0	40.0	31.0	41.0
21	37.0687636460106	39.0	36.0	40.0	31.0	41.0
22	36.974692268059854	39.0	36.0	40.0	31.0	41.0
23	36.77370176333754	39.0	35.0	40.0	30.0	41.0
24	36.97402509516659	39.0	36.0	40.0	31.0	41.0
25	36.93102212797015	39.0	36.0	40.0	31.0	41.0
26	36.72832279709433	39.0	35.0	40.0	30.0	41.0
27	36.65975178843437	39.0	35.0	40.0	30.0	41.0
28	36.472717527632454	39.0	35.0	40.0	30.0	41.0
29	36.30365998511212	38.0	35.0	40.0	30.0	41.0
30	36.11944678206363	38.0	35.0	40.0	29.0	41.0
31	35.85217117655338	38.0	35.0	40.0	29.0	41.0
32	35.71064084717257	38.0	34.0	40.0	28.0	41.0
33	35.51719931862841	38.0	34.0	40.0	27.0	41.0
34	35.399690534738866	38.0	34.0	40.0	27.0	41.0
35	35.150415312012306	38.0	34.0	40.0	26.0	41.0
36	34.99732217475944	38.0	33.0	40.0	25.0	41.0
37	34.83367259372492	38.0	33.0	40.0	25.0	41.0
38	34.73096740899857	38.0	33.0	40.0	24.0	41.0
39	34.62883349365688	38.0	33.0	40.0	24.0	41.0
40	34.49217431521366	38.0	33.0	40.0	23.0	41.0
41	34.276547523509855	38.0	33.0	40.0	22.0	41.0
42	34.04738006975631	38.0	33.0	40.0	21.0	41.0
43	33.69859312459028	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	2.0
14	14.0
15	20.0
16	35.0
17	104.0
18	255.0
19	585.0
20	1174.0
21	2469.0
22	4351.0
23	7339.0
24	11854.0
25	17710.0
26	25437.0
27	34870.0
28	47003.0
29	60895.0
30	76628.0
31	93771.0
32	113779.0
33	139099.0
34	167351.0
35	201668.0
36	253470.0
37	332647.0
38	420832.0
39	395306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06613148910748	21.683990881278515	14.086025032061247	23.163852597552758
2	19.303798650791247	24.060737228122893	34.27911076274012	22.356353358345743
3	19.76488279221197	24.886420768963465	32.754286492426736	22.59440994639783
4	14.065474280215106	16.822720911241095	36.15101439756613	32.96079041097767
5	12.347348392536798	39.14507211653231	34.84913464728059	13.658444843650294
6	32.13311406995808	37.84352449960995	13.521938031387434	16.501423399044533
7	25.866131156974113	33.167626462891775	21.55014113592101	19.416101244213095
8	27.209652127667084	34.37854318833913	18.871651628636705	19.54015305535708
9	24.67086621626615	14.303738451619171	21.626988492824466	39.39840683929021
10	16.521475950845925	27.896254822680227	33.344404445439004	22.23786478103485
11	33.22587435145771	22.84085290186995	23.210807951770914	20.72246479490142
12	20.978581134575872	27.77162177815069	30.622654567601803	20.627142519671636
13	31.230707722225244	21.27600656129459	26.03091082177682	21.462374894703345
14	20.437411335960785	23.408219719505073	27.733177341363763	28.42119160317038
15	22.70322513950639	29.895822218974693	25.543754211347252	21.85719843017166
16	21.746681053576847	28.62067920442435	26.604961823345736	23.027677918653065
17	21.73912501956473	27.227296712585485	27.11121610215758	23.922362165692203
18	22.691019238409897	26.003758919337677	29.445906061890557	21.859315780361868
19	22.674204986899415	25.456776786867113	30.501467406715154	21.367550819518318
20	23.492041877035096	25.077605036138184	30.263493852003865	21.166859234822855
21	22.295697502896825	25.880869574964784	30.709175308707582	21.11425761343081
22	21.308887764248418	26.318787414304403	30.262165318551183	22.110159502895996
23	22.177665608960293	26.336390482552414	31.085939092553527	20.400004815933766
24	22.145573222743995	26.562863919563945	28.76187740044199	22.529685457250075
25	21.992791875685803	26.623395225001673	29.949918440501005	21.43389445881152
26	21.647995928044967	27.38883807709729	29.94672165688049	21.01644433797725
27	20.855857856884562	27.31647452059663	29.486343298856504	22.341324323662302
28	21.22963244046198	26.994097574969768	29.793566659788738	21.982703324779514
29	21.70396039973911	26.94473425386863	29.180116338013782	22.171189008378477
30	21.45822322766372	27.101210584592085	30.75621369626653	20.684352491477664
31	21.949489988462517	27.039641362394452	30.441517334662976	20.569351314480063
32	21.483548396605432	26.733082268188557	29.654029130586952	22.129340204619062
33	21.161628134352927	26.82500017644585	30.18303454477594	21.83033714442529
34	20.623156919313594	27.110053635386482	30.712994842384038	21.553794602915882
35	20.295714939898392	28.773585101493726	29.79638979337568	21.134310165232193
36	22.31226265438493	26.790292239994585	29.544964837456007	21.352480268164477
37	21.243291425022345	27.293142151833937	29.609149609888608	21.85441681325511
38	21.107241296133843	25.9935043017498	31.06019875690785	21.8390556452085
39	20.527087344016678	25.955931715041196	31.615650290139254	21.90133065080287
40	19.7258156053691	26.367444952008807	31.399805120749157	22.506934321872933
41	20.761448948403498	25.335382042628485	31.48997932884981	22.413189680118208
42	19.64261619789502	27.124003236639627	30.92120094442122	22.312179621044137
43	20.028887299261708	26.73914370206641	30.379408395750186	22.852560602921695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	163.0
1	321.5
2	480.0
3	1069.5
4	1659.0
5	1659.0
6	2712.5
7	3766.0
8	4269.0
9	4772.0
10	7529.5
11	10287.0
12	10287.0
13	19908.0
14	29529.0
15	45578.0
16	61627.0
17	55774.5
18	49922.0
19	49922.0
20	53786.5
21	57651.0
22	46804.5
23	35958.0
24	40636.0
25	45314.0
26	45314.0
27	48833.0
28	52352.0
29	56601.0
30	60850.0
31	67961.5
32	75073.0
33	75073.0
34	84262.5
35	93452.0
36	101946.0
37	110440.0
38	117126.5
39	123813.0
40	123813.0
41	127227.0
42	130641.0
43	139044.0
44	147447.0
45	153981.5
46	160516.0
47	160516.0
48	200103.5
49	239691.0
50	225956.5
51	212222.0
52	194003.5
53	175785.0
54	175785.0
55	158463.5
56	141142.0
57	121854.5
58	102567.0
59	90804.0
60	79041.0
61	79041.0
62	70138.5
63	61236.0
64	53152.0
65	45068.0
66	38800.0
67	32532.0
68	32532.0
69	27785.5
70	23039.0
71	19577.0
72	16115.0
73	13290.5
74	10466.0
75	10466.0
76	8354.5
77	6243.0
78	4930.0
79	3617.0
80	2797.5
81	1978.0
82	1978.0
83	1516.0
84	1054.0
85	809.5
86	565.0
87	429.5
88	294.0
89	294.0
90	237.0
91	180.0
92	125.5
93	71.0
94	52.5
95	34.0
96	34.0
97	25.5
98	17.0
99	9.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2408671.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.02312293429656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.77626633976074	40.40681101287332
2	11.162389422077872	11.167551566021857
3	3.3356172965394655	5.005739822596789
4	1.4290610056301298	2.859443774609818
5	0.7316321165581271	1.8299261654633385
6	0.45320121151198156	1.3602323950461594
7	0.3187647686193408	1.1161926445437518
8	0.22822928643838922	0.9133393322171459
9	0.17634466660213624	0.7939179842621581
>10	1.1657394654285016	11.378004681233257
>50	0.12321221671116553	4.271390454059378
>100	0.08787485911955262	8.64061294237951
>500	0.0070837451801774405	2.4288888911472792
>1k	0.004000232572335469	3.6135053154790167
>5k	2.500145357709668E-4	0.7067844474282265
>10k+	3.3335271436128907E-4	3.5076585706388457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29132	1.2094636419834837	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24416	1.0136710243947804	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19314	0.8018529720331253	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11278	0.4682250087288799	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5998	0.24901698903669287	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5844	0.2426234217956707	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5112	0.2122332190656175	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4866	0.2020201181481406	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4703	0.19525290087355227	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4595	0.1907691004707575	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3135	0.1301547616922361	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3097	0.12857712821717868	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3061	0.12708252808291376	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2876	0.11940194405960797	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2589	0.10748665965588493	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2585	0.10732059297429994	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2569	0.10665632624795997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.1516670396247555E-5	0.0	0.0	0.0	0.0
3	4.1516670396247555E-5	0.0	0.0	0.0	0.0
4	4.1516670396247555E-5	4.1516670396247555E-5	0.0	0.0	0.0
5	4.1516670396247555E-5	1.6606668158499022E-4	0.0	0.0	0.0
6	8.303334079249511E-5	1.6606668158499022E-4	0.0	0.0	0.0
7	8.303334079249511E-5	1.6606668158499022E-4	0.0	0.0	0.0
8	8.303334079249511E-5	1.6606668158499022E-4	0.0	0.0	0.0
9	8.303334079249511E-5	1.6606668158499022E-4	0.0	1.6606668158499022E-4	0.0
10	8.303334079249511E-5	1.6606668158499022E-4	0.0	4.1516670396247557E-4	0.0
11	8.303334079249511E-5	1.6606668158499022E-4	0.0	6.642667263399609E-4	0.0
12	8.303334079249511E-5	1.6606668158499022E-4	0.0	8.303334079249511E-4	0.0
13	8.303334079249511E-5	2.0758335198123778E-4	0.0	8.303334079249511E-4	0.0
14	8.303334079249511E-5	2.0758335198123778E-4	0.0	9.548834191136938E-4	0.0
15	8.303334079249511E-5	2.0758335198123778E-4	0.0	0.0012039834414911792	0.0
16	1.6606668158499022E-4	2.0758335198123778E-4	0.0	0.0021173501902086254	0.0
17	1.6606668158499022E-4	2.0758335198123778E-4	0.0	0.002906166927737329	0.0
18	1.6606668158499022E-4	2.906166927737329E-4	0.0	0.0030307169389260716	0.0
19	1.6606668158499022E-4	2.906166927737329E-4	0.0	0.004110150369228508	0.0
20	1.6606668158499022E-4	2.906166927737329E-4	0.0	0.006767217274588352	0.0
21	1.6606668158499022E-4	2.906166927737329E-4	0.0	0.011126467666194345	0.0
22	1.6606668158499022E-4	2.906166927737329E-4	0.0	0.01685576818087651	0.0
23	1.6606668158499022E-4	3.3213336316998044E-4	0.0	0.02005255180138757	0.0
24	1.6606668158499022E-4	3.3213336316998044E-4	0.0	0.02582336898646598	0.0
25	1.6606668158499022E-4	3.3213336316998044E-4	0.0	0.03126205280837441	0.0
26	1.6606668158499022E-4	3.3213336316998044E-4	0.0	0.041392120385058816	0.0
27	1.6606668158499022E-4	3.3213336316998044E-4	0.0	0.10549385947686504	0.0
28	1.6606668158499022E-4	3.3213336316998044E-4	0.0	0.2217405365863582	0.0
29	1.6606668158499022E-4	3.3213336316998044E-4	0.0	0.36758859968837587	0.0
30	1.6606668158499022E-4	3.7365003356622803E-4	0.0	0.5754210516919911	0.0
31	1.6606668158499022E-4	3.7365003356622803E-4	0.0	0.8180029568172656	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8490	0.0	21.921083	1
GTATTGG	825	0.0	21.303032	1
TCTAACG	85	1.246468E-6	19.588236	2
ATTGGAC	1060	0.0	17.103773	3
GTATACT	190	1.8189894E-12	16.552631	4
CTCTAAT	485	0.0	16.402061	1
CTAATAC	600	0.0	16.033333	3
TAGGTCG	140	3.4780896E-8	15.857143	21
CGGTATA	105	9.351525E-6	15.857142	28
TATACCG	105	9.351525E-6	15.857142	5
CTGTGCG	200	3.6379788E-12	15.724999	9
TTGGACC	1410	0.0	15.482269	4
TGGACCC	1300	0.0	15.36923	5
CGGGTAA	280	0.0	15.196429	26
GGACCCT	1315	0.0	15.193916	6
AATACTG	710	0.0	15.112676	5
TCGGGTA	270	0.0	15.074075	25
CATTGCG	150	8.112329E-8	14.8	29
GTCGTCA	165	1.6474587E-8	14.575757	24
GTTATAC	115	2.211722E-5	14.478261	3
>>END_MODULE