FastQCFastQC Report
Wed 25 May 2016
SRR2088493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429258
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT324701.3366221290616311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT287941.1853002027779675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT229370.9441977756170814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123880.5099499517959805No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG82800.3408448176356731No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG76310.3141288409876596No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA68590.2823495898747684No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA68310.2811969745494303No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA65120.2680653928071864No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG61820.2544809979014168No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA37450.15416229976396084No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37440.15412113493091306No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA36500.15025164062442112No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC35590.14650564081707254No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33460.137737531377894No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33290.13703772921608162No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA29310.12065412566306254No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28310.11653764235828389No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG26490.10904564274358672No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG26250.10805768675043984No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG25580.10529964293623814No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC25260.10398236827870898No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC25090.1032825661168966No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA24930.10262392878813201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93300.023.9528391
GTATTGG10200.020.1323531
GTATTAG5550.016.6666661
ATTGGAC13950.015.9139793
TGGACCC15750.015.3873015
GTATCAA145700.015.3764592
TACCCCG3150.015.2698425
TAGCGAA4000.015.26250110
GGACCCT16100.015.1677026
TTACACC2450.015.102044
TTGGACC17350.014.9279554
TCTAACG1001.09415974E-414.82
GGTAAGA2800.014.5357152
CTAGGAC1803.3360266E-914.3888893
TACGCGA1051.6568285E-414.0952395
CCGGTCT6350.013.9842529
TCTAATA6400.013.8750012
GTATACT1601.7857565E-713.8750014
TTCGTTA2304.0017767E-1113.67391230
TCACGTA6300.013.50793725
Produced by FastQC (version 0.11.5)