##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2429258
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28145301981099	31.0	31.0	33.0	30.0	34.0
2	31.41138364060137	31.0	31.0	34.0	30.0	34.0
3	31.549926356113676	31.0	31.0	34.0	30.0	34.0
4	35.2723930517055	37.0	35.0	37.0	33.0	37.0
5	35.14380852095578	37.0	35.0	37.0	32.0	37.0
6	35.23283117725659	37.0	35.0	37.0	32.0	37.0
7	35.05316479352955	37.0	35.0	37.0	32.0	37.0
8	35.11756388164616	37.0	35.0	37.0	32.0	37.0
9	36.62971450541688	39.0	35.0	39.0	32.0	39.0
10	36.48885338650732	38.0	35.0	39.0	32.0	39.0
11	36.5181458700558	38.0	35.0	39.0	32.0	39.0
12	36.36083898869531	38.0	35.0	39.0	32.0	39.0
13	36.389034429443065	38.0	35.0	39.0	32.0	39.0
14	37.288481091757234	39.0	36.0	40.0	32.0	41.0
15	37.31597961188149	39.0	36.0	40.0	32.0	41.0
16	37.164466269124155	39.0	36.0	40.0	31.0	41.0
17	37.28022918932448	39.0	36.0	40.0	32.0	41.0
18	37.1549394918119	39.0	36.0	40.0	31.0	41.0
19	37.246894730819044	39.0	36.0	40.0	31.0	41.0
20	37.15715415982987	39.0	36.0	40.0	31.0	41.0
21	37.00232293152889	39.0	36.0	40.0	31.0	41.0
22	36.91452575230791	39.0	36.0	40.0	31.0	41.0
23	36.71369199977936	39.0	35.0	40.0	30.0	41.0
24	36.90138552595072	39.0	36.0	40.0	30.0	41.0
25	36.85873834726488	39.0	36.0	40.0	30.0	41.0
26	36.648989526843174	39.0	35.0	40.0	30.0	41.0
27	36.578908868469306	39.0	35.0	40.0	30.0	41.0
28	36.37178265956107	38.0	35.0	40.0	30.0	41.0
29	36.201012407903974	38.0	35.0	40.0	30.0	41.0
30	36.01269235297362	38.0	35.0	40.0	29.0	41.0
31	35.719661312219614	38.0	34.0	40.0	27.0	41.0
32	35.58452292840036	38.0	34.0	40.0	27.0	41.0
33	35.377420183446965	38.0	34.0	40.0	27.0	41.0
34	35.260201263101735	38.0	34.0	40.0	26.0	41.0
35	35.00260079415196	38.0	33.0	40.0	25.0	41.0
36	34.8362664649041	38.0	33.0	40.0	25.0	41.0
37	34.67420051719496	38.0	33.0	40.0	24.0	41.0
38	34.55503779343322	38.0	33.0	40.0	24.0	41.0
39	34.44266809042102	38.0	33.0	40.0	23.0	41.0
40	34.28552792663439	38.0	33.0	40.0	23.0	41.0
41	34.06073747621702	38.0	33.0	40.0	21.0	41.0
42	33.80817228964565	37.0	33.0	40.0	20.0	41.0
43	33.46608182416195	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	4.0
14	10.0
15	13.0
16	41.0
17	112.0
18	252.0
19	596.0
20	1310.0
21	2518.0
22	4705.0
23	7963.0
24	12775.0
25	19049.0
26	27238.0
27	37630.0
28	49919.0
29	64523.0
30	79852.0
31	96708.0
32	117723.0
33	141562.0
34	170381.0
35	204462.0
36	257410.0
37	336280.0
38	417920.0
39	378297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15758803717019	21.682505522262353	14.207218829782592	22.952687610784857
2	19.512172029483903	23.89091648561001	34.33217056401585	22.264740920890247
3	20.093090153454266	24.71499527839365	32.84278573951387	22.34912882863821
4	14.118426284898517	16.593626531228878	35.77108730320123	33.51685988067138
5	12.239869128762775	39.117211922323605	34.786877309861694	13.856041639051925
6	32.439411540478616	37.22000709681721	13.060695899735638	17.27988546296853
7	25.83315563847068	33.35429995496567	21.06639146603613	19.74615294052752
8	27.561378824315902	34.16516483634097	18.428219645669582	19.84523669367354
9	24.55926871497387	14.187789028584035	21.61787673437733	39.635065522064764
10	16.704195272795232	27.676105214020087	33.04416410278365	22.575535410401034
11	33.29654569419963	22.330686983432802	23.409576092782242	20.96319122958533
12	21.31803209045725	27.451756873909645	30.50626981572151	20.723941219911595
13	31.940411434273347	20.951912065330237	26.144238281812797	20.963438218583615
14	20.573030941958407	23.180576126537403	27.589165086623158	28.657227844881028
15	22.650331912048866	29.577056039333822	25.820188716060628	21.952423332556688
16	21.747010815648235	28.31959388422308	26.72523873544926	23.20815656467942
17	21.909941224851373	26.589806434722043	26.792872556146776	24.70737978427981
18	23.051400880433448	25.32826896113957	29.615010015403882	22.005320143023095
19	22.96989451099883	24.490893927281498	30.844603578541268	21.6946079831784
20	23.894333166752975	24.054382037642768	30.731235628327664	21.320049167276593
21	22.582862750683542	25.142245080596627	31.27341764440006	21.00147452431977
22	21.24574664362534	25.4682705583351	30.77783421933776	22.50814857870181
23	22.2451464603595	25.729008610859776	31.943786950583263	20.082057978197458
24	22.336203071061206	25.810021002297823	28.963329543424372	22.890446383216602
25	22.067273216760015	26.044701715503255	30.308061144596415	21.579963923140316
26	21.876680039748763	26.835766312182567	30.256563938453635	21.030989709615035
27	20.811910468134716	26.85165593773901	29.73920431670905	22.59722927741722
28	21.256819983715193	26.39826646655069	30.02365331306926	22.32126023666486
29	21.725646267296433	26.296301175091326	29.339699611980286	22.638352945631958
30	21.623763305503164	26.43021037699577	31.24974786539758	20.69627845210348
31	22.28906933722149	26.24472163928245	30.93635999140478	20.52984903209128
32	21.87400432560066	26.055610396260914	29.772753655642997	22.29763162249543
33	21.402543492704357	26.104884701419117	30.50532302456141	21.987248781315117
34	20.555371228580906	26.804110555568823	31.14831771676784	21.492200499082436
35	20.2365495966258	28.962053433599888	29.783044863904944	21.018352105869365
36	22.694954591072666	26.29712447175228	29.65407544196623	21.353845495208827
37	21.53562939794785	27.161544800922748	29.61389856491159	21.688927236217808
38	21.353804330375777	25.463207283870222	31.21763929562031	21.965349090133696
39	20.721389000262633	25.38001315628064	31.977500948849404	21.921096894607324
40	19.614343145108506	26.097804350134897	31.48504605109873	22.802806453657865
41	21.0911726955309	24.978779528563866	31.521765082177357	22.40828269372788
42	19.82259603549726	27.046736081552474	30.883833664435805	22.24683421851446
43	20.261248496454474	26.75574187673767	30.054609267521194	22.928400359286663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	330.0
2	487.0
3	1066.5
4	1646.0
5	1646.0
6	2781.0
7	3916.0
8	4458.5
9	5001.0
10	7843.5
11	10686.0
12	10686.0
13	20857.0
14	31028.0
15	48777.5
16	66527.0
17	60331.0
18	54135.0
19	54135.0
20	57227.0
21	60319.0
22	46667.0
23	33015.0
24	36009.0
25	39003.0
26	39003.0
27	42434.5
28	45866.0
29	49799.5
30	53733.0
31	59908.5
32	66084.0
33	66084.0
34	75199.0
35	84314.0
36	93652.5
37	102991.0
38	109691.5
39	116392.0
40	116392.0
41	121088.5
42	125785.0
43	134922.0
44	144059.0
45	153856.0
46	163653.0
47	163653.0
48	216403.5
49	269154.0
50	250499.0
51	231844.0
52	211498.5
53	191153.0
54	191153.0
55	168539.0
56	145925.0
57	123882.5
58	101840.0
59	90078.5
60	78317.0
61	78317.0
62	69237.0
63	60157.0
64	52547.5
65	44938.0
66	38788.0
67	32638.0
68	32638.0
69	28044.5
70	23451.0
71	19845.5
72	16240.0
73	13336.0
74	10432.0
75	10432.0
76	8469.5
77	6507.0
78	5142.5
79	3778.0
80	2884.0
81	1990.0
82	1990.0
83	1519.5
84	1049.0
85	776.0
86	503.0
87	373.5
88	244.0
89	244.0
90	207.5
91	171.0
92	120.0
93	69.0
94	47.5
95	26.0
96	26.0
97	19.5
98	13.0
99	9.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2429258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.29798596148714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.04231092014908	37.05797787306051
2	11.18909145431327	10.36064798147183
3	3.4674508750613597	4.816079758072115
4	1.4952520487296899	2.769086334438883
5	0.8541947261586349	1.9773747720034418
6	0.5244184665831986	1.4567711282288127
7	0.3693260115620418	1.1969335348958015
8	0.2584014474951584	0.957077327084704
9	0.20748992354450727	0.8645729010672275
>10	1.3412341537433619	12.00903348249543
>50	0.13681081729630015	4.357869357592933
>100	0.09841370803275737	8.956804354839024
>500	0.009058334370126358	2.7766867675677345
>1k	0.005650248171464944	4.67619945422044
>5k	5.381188734728519E-4	1.7562166983337641
>10k+	3.587459156485679E-4	4.0106682746272595
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32470	1.3366221290616311	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28794	1.1853002027779675	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22937	0.9441977756170814	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12388	0.5099499517959805	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8280	0.3408448176356731	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7631	0.3141288409876596	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6859	0.2823495898747684	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6831	0.2811969745494303	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6512	0.2680653928071864	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6182	0.2544809979014168	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3745	0.15416229976396084	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3744	0.15412113493091306	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3650	0.15025164062442112	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3559	0.14650564081707254	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3346	0.137737531377894	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3329	0.13703772921608162	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2931	0.12065412566306254	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2831	0.11653764235828389	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2649	0.10904564274358672	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2625	0.10805768675043984	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2558	0.10529964293623814	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2526	0.10398236827870898	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2509	0.1032825661168966	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2493	0.10262392878813201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	8.232966609557322E-5	0.0	0.0	0.0	0.0
7	8.232966609557322E-5	0.0	0.0	0.0	0.0
8	1.2349449914335982E-4	0.0	0.0	0.0	0.0
9	1.6465933219114644E-4	0.0	0.0	3.293186643822929E-4	0.0
10	1.6465933219114644E-4	0.0	0.0	4.939779965734393E-4	4.116483304778661E-5
11	2.4698899828671963E-4	0.0	0.0	5.763076626690125E-4	4.116483304778661E-5
12	2.8815383133450626E-4	0.0	0.0	9.056263270513054E-4	4.116483304778661E-5
13	2.8815383133450626E-4	0.0	0.0	9.879559931468785E-4	4.116483304778661E-5
14	4.528131635256527E-4	0.0	0.0	0.0011937801583858116	4.116483304778661E-5
15	4.939779965734393E-4	0.0	0.0	0.0015642636558158912	4.116483304778661E-5
16	4.939779965734393E-4	0.0	0.0	0.0022640658176282633	4.116483304778661E-5
17	4.939779965734393E-4	0.0	0.0	0.0029638679794406356	4.116483304778661E-5
18	5.351428296212259E-4	0.0	0.0	0.003457845976014075	4.116483304778661E-5
19	6.174724957167991E-4	0.0	0.0	0.00436347230306538	4.116483304778661E-5
20	6.586373287645858E-4	0.0	0.0	0.007903647945175028	4.116483304778661E-5
21	6.586373287645858E-4	0.0	0.0	0.012925757577004995	4.116483304778661E-5
22	6.586373287645858E-4	0.0	0.0	0.020582416523893303	4.116483304778661E-5
23	6.586373287645858E-4	0.0	0.0	0.02535753715743655	4.116483304778661E-5
24	6.586373287645858E-4	0.0	0.0	0.03190274561203462	4.116483304778661E-5
25	6.586373287645858E-4	0.0	0.0	0.03754232773958139	4.116483304778661E-5
26	6.586373287645858E-4	0.0	0.0	0.04622810751266436	4.116483304778661E-5
27	6.586373287645858E-4	0.0	0.0	0.10686390659205404	4.116483304778661E-5
28	6.586373287645858E-4	0.0	0.0	0.22755919708816438	4.116483304778661E-5
29	6.586373287645858E-4	0.0	0.0	0.3771522003838209	4.116483304778661E-5
30	6.586373287645858E-4	0.0	0.0	0.5946671782083254	4.116483304778661E-5
31	6.586373287645858E-4	0.0	0.0	0.834699319709969	4.116483304778661E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9330	0.0	23.952839	1
GTATTGG	1020	0.0	20.132353	1
GTATTAG	555	0.0	16.666666	1
ATTGGAC	1395	0.0	15.913979	3
TGGACCC	1575	0.0	15.387301	5
GTATCAA	14570	0.0	15.376459	2
TACCCCG	315	0.0	15.269842	5
TAGCGAA	400	0.0	15.262501	10
GGACCCT	1610	0.0	15.167702	6
TTACACC	245	0.0	15.10204	4
TTGGACC	1735	0.0	14.927955	4
TCTAACG	100	1.09415974E-4	14.8	2
GGTAAGA	280	0.0	14.535715	2
CTAGGAC	180	3.3360266E-9	14.388889	3
TACGCGA	105	1.6568285E-4	14.095239	5
CCGGTCT	635	0.0	13.984252	9
TCTAATA	640	0.0	13.875001	2
GTATACT	160	1.7857565E-7	13.875001	4
TTCGTTA	230	4.0017767E-11	13.673912	30
TCACGTA	630	0.0	13.507937	25
>>END_MODULE