FastQCFastQC Report
Wed 25 May 2016
SRR2088492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536788
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77471.443214080791672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67401.2556167425501314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39250.7312011445859445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29100.5421134600624455No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG17950.3343964470144638No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG15830.2949022705425605No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA13780.2567121470673711No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA9760.1818222463989508No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9200.1713898224252405No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG9140.17027206271377154No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.16878171643181292No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.16505585072691642No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC6280.11699218313375112No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.10264760016989947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTG250.005493765729.65
GGTATCA24000.023.2020841
ATCACGT1054.0017767E-1122.9047624
TCACGTA1054.0017767E-1122.9047625
GGGTGTA450.00382319220.5555557
CACGTAG1254.110916E-1019.2426
GTCATAG500.00703011718.51
GATCACG1351.1477823E-917.81481423
CGTAGGA1351.1477823E-917.81481428
ATTACTC1900.017.5263163
ATACCAT752.0646804E-417.2666666
GTATCAA32450.017.1602462
TTAATAC650.001578451417.0769233
TATGCGG650.001578451417.0769235
AGGGCTA650.001578451417.0769235
ACGTAGG1452.9667717E-916.58620827
TAGTACC904.438858E-516.4444454
GTATTGG1352.2115273E-816.4444431
ACAAACG803.378365E-416.187522
TAATACC1059.324474E-615.8571424
Produced by FastQC (version 0.11.5)