##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088492_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536788
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.656188290349263	31.0	30.0	31.0	28.0	34.0
2	30.79275617189654	31.0	31.0	33.0	27.0	34.0
3	30.832758929037162	31.0	30.0	34.0	27.0	34.0
4	34.60324746454839	35.0	35.0	37.0	32.0	37.0
5	34.35707951742587	35.0	35.0	37.0	30.0	37.0
6	34.601671423355214	35.0	35.0	37.0	31.0	37.0
7	34.44190630192925	35.0	35.0	37.0	30.0	37.0
8	34.548194072892834	35.0	35.0	37.0	30.0	37.0
9	35.96048719419957	37.0	35.0	39.0	30.0	39.0
10	35.683273471091006	37.0	35.0	39.0	30.0	39.0
11	35.81771388332079	37.0	35.0	39.0	30.0	39.0
12	35.58303091723362	37.0	35.0	39.0	30.0	39.0
13	35.67615334172895	37.0	35.0	39.0	30.0	39.0
14	36.40090314984687	38.0	35.0	40.0	30.0	41.0
15	36.48025104883119	38.0	35.0	40.0	30.0	41.0
16	36.26802201241458	38.0	34.0	40.0	30.0	41.0
17	36.397076685767935	38.0	35.0	40.0	30.0	41.0
18	36.250536524661506	38.0	35.0	40.0	30.0	41.0
19	36.373551569707224	38.0	35.0	40.0	30.0	41.0
20	36.27341706595527	38.0	34.0	40.0	30.0	41.0
21	36.10089830622145	38.0	34.0	40.0	29.0	41.0
22	35.995974202105856	38.0	34.0	40.0	29.0	41.0
23	35.787457990864176	38.0	34.0	40.0	27.0	41.0
24	35.96529356095889	38.0	34.0	40.0	29.0	41.0
25	35.90812015171725	38.0	34.0	40.0	29.0	41.0
26	35.62260333688533	38.0	34.0	40.0	27.0	41.0
27	35.53788273955453	38.0	34.0	40.0	27.0	41.0
28	35.30661639231876	38.0	34.0	40.0	27.0	41.0
29	35.12729420180779	38.0	33.0	40.0	26.0	41.0
30	34.93580705977034	37.0	33.0	40.0	26.0	41.0
31	34.61964686244849	37.0	33.0	40.0	25.0	41.0
32	34.467657622748646	37.0	33.0	40.0	25.0	41.0
33	34.252596928396315	37.0	33.0	40.0	24.0	41.0
34	34.116315938508315	37.0	33.0	40.0	24.0	41.0
35	33.828243179802826	36.0	32.0	39.0	23.0	40.0
36	33.68812082237308	36.0	32.0	39.0	23.0	40.0
37	33.48894163058787	36.0	31.0	39.0	21.0	40.0
38	33.360717825286706	36.0	31.0	39.0	21.0	40.0
39	33.240910750612905	36.0	31.0	39.0	20.0	40.0
40	33.06260013264082	36.0	31.0	39.0	18.0	40.0
41	32.830774905549305	36.0	31.0	39.0	18.0	40.0
42	32.560763280848306	36.0	30.0	39.0	15.0	40.0
43	32.19280609849699	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	5.0
15	14.0
16	25.0
17	44.0
18	149.0
19	293.0
20	549.0
21	1082.0
22	1904.0
23	2909.0
24	4460.0
25	6282.0
26	8590.0
27	11503.0
28	14654.0
29	18475.0
30	22842.0
31	26963.0
32	31893.0
33	38116.0
34	43893.0
35	51936.0
36	64695.0
37	76426.0
38	77524.0
39	31559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.23408869050724	25.464429160115355	15.697631094584827	20.60385105479258
2	22.507582136709463	26.068578284164325	30.93884364031983	20.48499593880638
3	23.089562359814302	25.401648322987846	31.824854504944224	19.68393481225363
4	17.58999828610178	19.361647428780078	33.26415642674576	29.78419785837239
5	12.10049404979247	41.10132864371037	34.04304865235437	12.755128654142789
6	31.990469235526874	38.6424435717639	12.989485606980782	16.377601585728442
7	25.11810994284522	33.977100829377704	21.348092729345662	19.55669649843141
8	27.651139742319124	35.47750694873954	18.34858454361871	18.522768765322624
9	24.683674001654285	14.042228961899298	21.590460293449183	39.68363674299724
10	16.624067602107353	29.332436641653686	32.99328598999978	21.050209766239185
11	33.77683554773952	22.7728637748981	22.787767237717684	20.6625334396447
12	20.022802298113966	28.832798050627062	31.083407229669813	20.060992421589155
13	32.084919931146	21.24488624932003	26.19153185242591	20.47866196710806
14	19.849363249551033	23.879073302681878	28.292361230131817	27.97920221763527
15	23.00256339560497	30.4554125651095	25.48398995506606	21.058034084219468
16	21.197381461582598	28.881606891361205	27.375798266727276	22.545213380328917
17	21.55096611697728	27.855689769517948	27.8754368577539	22.717907255750873
18	22.041662630312153	26.327339657369393	29.825927554267235	21.805070158051223
19	22.480197023778477	25.617003360730866	31.385388645051677	20.51741097043898
20	22.885384919185974	25.26621310461486	31.276593366468695	20.57180860973047
21	21.915542076201405	26.29846419815644	31.74903313784958	20.036960587792574
22	20.783251488483348	26.647577814705247	31.211949596488743	21.35722110032266
23	21.636102148334167	26.467432207873497	32.06032921749368	19.83613642629865
24	21.697392639179714	26.921242650729898	29.74638032146769	21.634984388622698
25	21.59306839944261	27.011036014217904	30.573522507954724	20.82237307838476
26	21.236689344769257	27.80781239521003	30.73578395940297	20.21971430061775
27	20.97438839914454	27.156158483423624	30.39170026155577	21.477752855876062
28	20.834482141925676	27.595065463460433	30.729077401134152	20.841374993479736
29	21.315863990998306	26.97657175644761	30.33096865056596	21.37659560198812
30	21.257554192716675	27.04251957942428	31.635953113705966	20.063973114153075
31	21.624179378078495	27.282092744249127	31.09719293277793	19.996534944894446
32	21.216569669962816	26.897583403503805	30.45019635312265	21.435650573410733
33	20.985007116403494	27.341147715671738	30.76838528432081	20.905459883603957
34	20.65787610751358	27.3189788147276	31.299693733839057	20.72345134391976
35	20.13066611027072	28.85478065828595	30.613389270997114	20.40116396044621
36	22.21603314530131	26.909692467044717	30.298367325648112	20.575907062005857
37	21.06585840219975	27.506576152969142	30.296318099510422	21.131247345320688
38	20.98053607755762	26.07081380358726	31.819079413101637	21.12957070575348
39	20.311929476813937	26.145144824399953	32.28686185235139	21.25606384643472
40	19.518692668241467	26.75152946787186	31.807715522701702	21.922062341184976
41	20.879751410240168	25.655938657347033	31.71233336065635	21.751976571756447
42	19.948657570586526	27.268865920996742	30.95859072855578	21.82388577986095
43	20.540697631094584	26.779473460658583	30.483542851181472	22.196286057065358
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	38.0
1	66.5
2	95.0
3	157.5
4	220.0
5	220.0
6	434.5
7	649.0
8	779.5
9	910.0
10	1532.5
11	2155.0
12	2155.0
13	4655.0
14	7155.0
15	11594.0
16	16033.0
17	13904.0
18	11775.0
19	11775.0
20	13385.0
21	14995.0
22	12075.5
23	9156.0
24	9941.5
25	10727.0
26	10727.0
27	11607.5
28	12488.0
29	13570.0
30	14652.0
31	16139.0
32	17626.0
33	17626.0
34	19746.5
35	21867.0
36	23909.0
37	25951.0
38	27225.5
39	28500.0
40	28500.0
41	29426.0
42	30352.0
43	31594.0
44	32836.0
45	34084.0
46	35332.0
47	35332.0
48	44346.5
49	53361.0
50	49257.0
51	45153.0
52	41404.0
53	37655.0
54	37655.0
55	33840.0
56	30025.0
57	25587.0
58	21149.0
59	18689.5
60	16230.0
61	16230.0
62	14304.5
63	12379.0
64	10800.0
65	9221.0
66	7802.5
67	6384.0
68	6384.0
69	5375.5
70	4367.0
71	3643.0
72	2919.0
73	2384.0
74	1849.0
75	1849.0
76	1493.0
77	1137.0
78	895.0
79	653.0
80	518.5
81	384.0
82	384.0
83	282.0
84	180.0
85	155.0
86	130.0
87	94.0
88	58.0
89	58.0
90	43.0
91	28.0
92	19.0
93	10.0
94	7.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	536788.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.94644858076287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41075239045941	57.17500080303834
2	6.144303526631986	7.858127790607446
3	1.570722509261794	3.013263785194684
4	0.7292340817149973	1.865277188389116
5	0.41373966010025715	1.32285909502117
6	0.2958770958545602	1.1352173697773449
7	0.20600223995655884	0.9221178151432845
8	0.16627202022250837	0.8506004153262556
9	0.1278782598070386	0.7359624508813406
>10	0.8061289562992062	9.962997205479178
>50	0.07943954549532743	3.5452684084215385
>100	0.04497679991906804	5.46404230385764
>500	0.002628514280984484	1.278933646539872
>1k	0.0014602857116580467	2.164737134498197
>5k	5.841142846632187E-4	2.7055945878246384
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7747	1.443214080791672	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6740	1.2556167425501314	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3925	0.7312011445859445	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2910	0.5421134600624455	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1795	0.3343964470144638	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1583	0.2949022705425605	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1378	0.2567121470673711	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	976	0.1818222463989508	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	920	0.1713898224252405	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	914	0.17027206271377154	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.16878171643181292	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.16505585072691642	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	628	0.11699218313375112	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	551	0.10264760016989947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.725865704896533E-4	0.0
10	0.0	0.0	0.0	3.725865704896533E-4	0.0
11	0.0	0.0	0.0	5.588798557344799E-4	0.0
12	0.0	0.0	0.0	5.588798557344799E-4	0.0
13	0.0	0.0	0.0	9.314664262241332E-4	0.0
14	0.0	0.0	0.0	9.314664262241332E-4	0.0
15	0.0	0.0	0.0	0.0013040529967137864	0.0
16	0.0	0.0	0.0	0.0014903462819586132	0.0
17	0.0	0.0	0.0	0.0016766395672034398	0.0
18	3.725865704896533E-4	0.0	0.0	0.0018629328524482664	0.0
19	3.725865704896533E-4	0.0	0.0	0.002049226137693093	0.0
20	3.725865704896533E-4	0.0	0.0	0.004843625416365492	0.0
21	3.725865704896533E-4	0.0	0.0	0.008010611265527545	0.0
22	3.725865704896533E-4	0.0	0.0	0.014717169534341304	0.0
23	3.725865704896533E-4	0.0	0.0	0.018070448668748185	0.0
24	3.725865704896533E-4	0.0	0.0	0.025335886793296424	0.0
25	3.725865704896533E-4	0.0	0.0	0.02924804578343778	0.0
26	3.725865704896533E-4	0.0	0.0	0.03483684434078258	0.0
27	3.725865704896533E-4	0.0	0.0	0.07451731409793065	0.0
28	3.725865704896533E-4	0.0	0.0	0.1589081723138371	0.0
29	3.725865704896533E-4	0.0	0.0	0.26155577248373657	0.0
30	3.725865704896533E-4	0.0	0.0	0.4178558388041461	0.0
31	3.725865704896533E-4	0.0	0.0	0.5980014456358935	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGTG	25	0.0054937657	29.6	5
GGTATCA	2400	0.0	23.202084	1
ATCACGT	105	4.0017767E-11	22.90476	24
TCACGTA	105	4.0017767E-11	22.90476	25
GGGTGTA	45	0.003823192	20.555555	7
CACGTAG	125	4.110916E-10	19.24	26
GTCATAG	50	0.007030117	18.5	1
GATCACG	135	1.1477823E-9	17.814814	23
CGTAGGA	135	1.1477823E-9	17.814814	28
ATTACTC	190	0.0	17.526316	3
ATACCAT	75	2.0646804E-4	17.266666	6
GTATCAA	3245	0.0	17.160246	2
TTAATAC	65	0.0015784514	17.076923	3
TATGCGG	65	0.0015784514	17.076923	5
AGGGCTA	65	0.0015784514	17.076923	5
ACGTAGG	145	2.9667717E-9	16.586208	27
TAGTACC	90	4.438858E-5	16.444445	4
GTATTGG	135	2.2115273E-8	16.444443	1
ACAAACG	80	3.378365E-4	16.1875	22
TAATACC	105	9.324474E-6	15.857142	4
>>END_MODULE