FastQCFastQC Report
Wed 25 May 2016
SRR2088491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604658
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63051.0427382090371746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53670.887609193957576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43500.7194149420002712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.4071723188976248No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG13210.2184706065246801No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12040.1991208253260521No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA11820.19548240492972888No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG11480.18985939158995663No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA10490.1734864998065022No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG9630.15926358371178417No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.11229488405015728No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA6730.11130258757843275No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA6380.10551419149337311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA601.3356548E-624.66666825
GTCGGGT551.9007262E-523.54545424
GGTATCA19750.023.324051
TACCCTC1301.8189894E-1221.3461535
CTACCCT909.4596544E-820.5555574
CTCTAAT2100.018.5000021
CGGGTAA701.2179847E-418.526
TAGACTA500.007031120418.55
ACGTTCG500.007031120418.522
TAGCGAA902.148785E-618.510
ACGGGTA609.229914E-418.55
CAGATCG500.007031120418.59
CTCTATA500.007031120418.52
CTAATAC2300.018.53
ATACAAC609.229914E-418.56
GGTAAAC1950.018.02564235
ATGCTAG1900.017.52631615
GATTAAT953.6006713E-617.5263161
AATACTG2850.017.5263165
ATACTGG2550.017.4117646
Produced by FastQC (version 0.11.5)