##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	604658
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.391113654330216	31.0	31.0	34.0	30.0	34.0
2	31.622442769300992	31.0	31.0	34.0	30.0	34.0
3	31.735751449579762	31.0	31.0	34.0	30.0	34.0
4	35.43815346857232	37.0	35.0	37.0	33.0	37.0
5	35.3066328403825	37.0	35.0	37.0	33.0	37.0
6	35.35817106529642	37.0	35.0	37.0	33.0	37.0
7	35.203116472452194	37.0	35.0	37.0	32.0	37.0
8	35.24622348501136	37.0	35.0	37.0	32.0	37.0
9	36.78640322297894	39.0	37.0	39.0	32.0	39.0
10	36.63259726986164	39.0	35.0	39.0	32.0	39.0
11	36.67738787876783	39.0	35.0	39.0	32.0	39.0
12	36.5319039853934	38.0	35.0	39.0	32.0	39.0
13	36.54615832420972	38.0	35.0	39.0	32.0	39.0
14	37.510301691203956	39.0	36.0	41.0	32.0	41.0
15	37.53848952631074	39.0	36.0	41.0	32.0	41.0
16	37.40730627892131	39.0	36.0	41.0	32.0	41.0
17	37.455260659744845	39.0	36.0	41.0	32.0	41.0
18	37.331063179516356	39.0	36.0	41.0	32.0	41.0
19	37.41218010842493	39.0	36.0	41.0	32.0	41.0
20	37.319901498036906	39.0	36.0	41.0	31.0	41.0
21	37.16495440397713	39.0	36.0	40.0	31.0	41.0
22	37.07764554508499	39.0	36.0	40.0	31.0	41.0
23	36.87532125598272	39.0	36.0	40.0	30.0	41.0
24	37.07516645773313	39.0	36.0	40.0	31.0	41.0
25	37.039895279645684	39.0	36.0	41.0	31.0	41.0
26	36.86722907825581	39.0	36.0	40.0	30.0	41.0
27	36.804193444889506	39.0	35.0	40.0	30.0	41.0
28	36.604483856990235	39.0	35.0	40.0	30.0	41.0
29	36.45372921552349	39.0	35.0	40.0	30.0	41.0
30	36.264678545557985	38.0	35.0	40.0	30.0	41.0
31	36.018736211213614	38.0	35.0	40.0	29.0	41.0
32	35.8599191609141	38.0	35.0	40.0	29.0	41.0
33	35.65387210621542	38.0	34.0	40.0	27.0	41.0
34	35.54518752749488	38.0	34.0	40.0	27.0	41.0
35	35.31857347459225	38.0	34.0	40.0	26.0	41.0
36	35.18241551422457	38.0	34.0	40.0	25.0	41.0
37	35.014083994588674	38.0	33.0	40.0	25.0	41.0
38	34.952301962431655	38.0	33.0	40.0	25.0	41.0
39	34.84238858991364	38.0	33.0	40.0	24.0	41.0
40	34.740056362439596	38.0	33.0	40.0	24.0	41.0
41	34.529585319304466	38.0	33.0	40.0	23.0	41.0
42	34.317400910928164	38.0	33.0	40.0	22.0	41.0
43	34.00381537993378	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	5.0
16	8.0
17	18.0
18	47.0
19	120.0
20	271.0
21	512.0
22	985.0
23	1626.0
24	2636.0
25	4101.0
26	5962.0
27	7930.0
28	10885.0
29	14387.0
30	18112.0
31	22329.0
32	27432.0
33	34016.0
34	41390.0
35	50537.0
36	63677.0
37	84278.0
38	107528.0
39	105862.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4846078278961	21.36066999857771	13.484978285245544	23.66974388828065
2	19.213836582001726	23.46202316019965	34.508432866182204	22.815707391616417
3	19.48390660505608	25.160173188810862	32.24020851456526	23.115711691567796
4	13.925723301436516	16.453102414918845	36.618882078794954	33.00229220484968
5	12.50177786451184	38.667478144670206	35.278620310985715	13.552123679832237
6	32.26815819851883	37.69932755375766	13.821862937396014	16.21065131032749
7	26.21498433825402	32.61016971577321	21.976059193792192	19.19878675218057
8	27.068690069427674	34.27044709571361	19.172325512934584	19.48853732192413
9	24.77681598523463	14.34827621564587	21.53796030152582	39.336947497593684
10	16.568704953874754	27.311802705000183	33.65786940716901	22.461622933956054
11	33.35339977309487	22.58053312781771	23.377181811867203	20.688885287220213
12	20.81292234618578	27.25292644767786	30.887212275368885	21.046938930767475
13	30.963122955455813	21.094569161410252	26.03570944236246	21.90659844077148
14	20.63976661186985	22.683731960877058	27.416655365512405	29.259846061740685
15	22.992005398092807	29.578538611909543	25.14760410016902	22.28185188982863
16	22.25357144038448	28.170800684023035	26.265922223802544	23.30970565178994
17	21.918175232941596	26.925468612008775	27.038259644294794	24.118096510754842
18	22.928167658411862	25.933502905774837	28.917338396250443	22.22099103956286
19	22.92568691723255	25.24964525401136	29.908807954248516	21.915859874507575
20	23.60805612428844	24.97742525526827	29.74408673994225	21.67043188050104
21	22.660247611046245	25.851969212348138	30.03218348223293	21.455599694372687
22	21.63487459026425	26.359528857635222	29.559023447965625	22.4465731041349
23	22.326009082820374	26.308094823850837	30.488639859226208	20.877256234102585
24	22.468072861022264	26.40864753298559	28.214130963288337	22.90914864270381
25	22.38703531583143	26.353740461550167	29.188731481267094	22.07049274135131
26	22.211068074845617	27.189254090742203	29.23090408131539	21.368773753096793
27	21.497441528930402	27.269960870442468	28.70416003757496	22.528437563052172
28	21.51001061757225	26.824419754638157	29.32947219750669	22.3360974302829
29	22.26217134313943	26.592222380254622	28.659010548111496	22.486595728494454
30	21.997724333424845	26.76355890437239	29.97975715197682	21.258959610225947
31	22.387862229557864	26.604791468896465	29.888962024814024	21.11838427673164
32	21.79496508770247	26.41989355966513	29.15383572201145	22.631305630620947
33	21.57599833294193	26.536157629602187	29.77286333762226	22.114980699833627
34	21.253171214140888	26.97309884265155	29.974464904127622	21.799265039079945
35	21.03321216290862	28.540596502485705	29.036579355602672	21.389611979003007
36	22.845310903022867	26.600326134773706	28.834647023606735	21.719715938596696
37	21.77197688610752	26.948787579094297	28.9730723814123	22.30616315338588
38	21.6275977494716	25.911507000651607	30.175404939651834	22.285490310224954
39	21.129464920665896	25.868672869622166	30.72943713636469	22.272425073347247
40	20.419807560637583	26.51052330408264	30.438363504658835	22.631305630620947
41	21.39440807861634	25.27643725874792	30.581584962077734	22.747569700558003
42	20.27840531341684	26.963175877934304	30.128105474499634	22.63031333414922
43	20.62388986832226	26.393597703164435	29.724902341488907	23.2576100870244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	74.0
2	111.0
3	289.5
4	468.0
5	468.0
6	718.0
7	968.0
8	1033.5
9	1099.0
10	1640.0
11	2181.0
12	2181.0
13	4251.0
14	6321.0
15	9756.0
16	13191.0
17	12119.5
18	11048.0
19	11048.0
20	11912.5
21	12777.0
22	10381.5
23	7986.0
24	8912.0
25	9838.0
26	9838.0
27	11076.0
28	12314.0
29	13652.5
30	14991.0
31	16848.0
32	18705.0
33	18705.0
34	21014.0
35	23323.0
36	25694.0
37	28065.0
38	29798.0
39	31531.0
40	31531.0
41	32958.0
42	34385.0
43	36558.0
44	38731.0
45	39843.5
46	40956.0
47	40956.0
48	49691.0
49	58426.0
50	55831.0
51	53236.0
52	49177.5
53	45119.0
54	45119.0
55	41176.0
56	37233.0
57	31872.5
58	26512.0
59	23867.5
60	21223.0
61	21223.0
62	18698.0
63	16173.0
64	14068.0
65	11963.0
66	10290.0
67	8617.0
68	8617.0
69	7343.5
70	6070.0
71	5182.0
72	4294.0
73	3593.5
74	2893.0
75	2893.0
76	2316.0
77	1739.0
78	1371.0
79	1003.0
80	763.5
81	524.0
82	524.0
83	412.0
84	300.0
85	221.5
86	143.0
87	109.0
88	75.0
89	75.0
90	64.0
91	53.0
92	35.5
93	18.0
94	14.0
95	10.0
96	10.0
97	7.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	604658.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.470038481422385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.25081047355015	52.15589828389445
2	7.601240463579212	9.192946066783596
3	2.1811827848274428	3.956886208005945
4	1.0396116929431738	2.514614363120416
5	0.619549274459628	1.8732084233855515
6	0.4430127383924913	1.6073398403012553
7	0.29582732884431917	1.252208297135066
8	0.23061425952173842	1.11562025181154
9	0.1713405181280508	0.9324870952167071
>10	1.0327456612695278	11.627168863010933
>50	0.07786177615151828	3.220214914116294
>100	0.050993967170229845	5.453011108142968
>500	0.0027416111381836357	1.0552236319872412
>1k	0.001919127796728545	2.1081262693401035
>5k	5.483222276367272E-4	1.9350463837478522
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6305	1.0427382090371746	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5367	0.887609193957576	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4350	0.7194149420002712	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2462	0.4071723188976248	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1321	0.2184706065246801	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1204	0.1991208253260521	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1182	0.19548240492972888	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1148	0.18985939158995663	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1049	0.1734864998065022	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	963	0.15926358371178417	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.11229488405015728	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	673	0.11130258757843275	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	638	0.10551419149337311	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	1.6538274528741866E-4
8	1.6538274528741866E-4	0.0	0.0	0.0	1.6538274528741866E-4
9	1.6538274528741866E-4	0.0	0.0	0.0	1.6538274528741866E-4
10	1.6538274528741866E-4	0.0	0.0	1.6538274528741866E-4	1.6538274528741866E-4
11	1.6538274528741866E-4	0.0	0.0	8.269137264370934E-4	1.6538274528741866E-4
12	1.6538274528741866E-4	0.0	0.0	8.269137264370934E-4	3.307654905748373E-4
13	1.6538274528741866E-4	0.0	0.0	8.269137264370934E-4	3.307654905748373E-4
14	1.6538274528741866E-4	0.0	0.0	8.269137264370934E-4	3.307654905748373E-4
15	1.6538274528741866E-4	0.0	0.0	0.0013230619622993493	3.307654905748373E-4
16	1.6538274528741866E-4	0.0	0.0	0.001984592943449024	4.96148235862256E-4
17	1.6538274528741866E-4	0.0	0.0	0.002976889415173536	4.96148235862256E-4
18	1.6538274528741866E-4	0.0	0.0	0.002976889415173536	4.96148235862256E-4
19	3.307654905748373E-4	0.0	0.0	0.003473037651035792	4.96148235862256E-4
20	3.307654905748373E-4	0.0	0.0	0.006946075302071584	4.96148235862256E-4
21	3.307654905748373E-4	0.0	0.0	0.012734471387131237	4.96148235862256E-4
22	3.307654905748373E-4	0.0	0.0	0.019019015708053148	4.96148235862256E-4
23	3.307654905748373E-4	0.0	0.0	0.024642029047825382	4.96148235862256E-4
24	3.307654905748373E-4	0.0	0.0	0.03224963533104664	4.96148235862256E-4
25	3.307654905748373E-4	0.0	0.0	0.03621882121794469	4.96148235862256E-4
26	3.307654905748373E-4	0.0	0.0	0.044322575737028205	4.96148235862256E-4
27	3.307654905748373E-4	0.0	0.0	0.0965835232478525	4.96148235862256E-4
28	3.307654905748373E-4	0.0	0.0	0.19498625669386663	6.615309811496747E-4
29	3.307654905748373E-4	0.0	0.0	0.31670795722540673	6.615309811496747E-4
30	3.307654905748373E-4	0.0	0.0	0.4792791958429393	6.615309811496747E-4
31	3.307654905748373E-4	0.0	0.0	0.6694693529234708	6.615309811496747E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	60	1.3356548E-6	24.666668	25
GTCGGGT	55	1.9007262E-5	23.545454	24
GGTATCA	1975	0.0	23.32405	1
TACCCTC	130	1.8189894E-12	21.346153	5
CTACCCT	90	9.4596544E-8	20.555557	4
CTCTAAT	210	0.0	18.500002	1
CGGGTAA	70	1.2179847E-4	18.5	26
TAGACTA	50	0.0070311204	18.5	5
ACGTTCG	50	0.0070311204	18.5	22
TAGCGAA	90	2.148785E-6	18.5	10
ACGGGTA	60	9.229914E-4	18.5	5
CAGATCG	50	0.0070311204	18.5	9
CTCTATA	50	0.0070311204	18.5	2
CTAATAC	230	0.0	18.5	3
ATACAAC	60	9.229914E-4	18.5	6
GGTAAAC	195	0.0	18.025642	35
ATGCTAG	190	0.0	17.526316	15
GATTAAT	95	3.6006713E-6	17.526316	1
AATACTG	285	0.0	17.526316	5
ATACTGG	255	0.0	17.411764	6
>>END_MODULE