FastQCFastQC Report
Wed 25 May 2016
SRR2088490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807132
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT166540.9215707541009732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT136140.7533483995635072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT113580.6285097048804404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62480.34574120761516036No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG34560.1912422556846982No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32690.18089436742861065No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32220.1782935612893801No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28140.15571635054882543No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA27140.15018272046535616No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG25790.14271231985267263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49400.021.7580991
CTAATAC4700.020.4680863
CTCTAAT4150.018.722891
TAATACT5300.018.1509444
TTAACGG2900.017.86206835
GTATTGG5650.016.3716811
TCTAATA4650.016.3118272
GTCTAGA1151.243081E-616.0869561
GTATTAG5150.015.8058241
ATGCGTA957.06325E-515.5789487
ATACTGG5250.015.5047626
CCGTTTA2750.015.47272727
ATGCTAG4500.015.21111115
AATACTG6250.015.0965
ACCGTTA3200.015.031258
CGCTAGA750.00410561514.81
TACTGGT5500.014.7999997
ATTAGAG4450.014.5505613
GACCGTT3250.014.2307697
GATGCTA4950.014.20202114
Produced by FastQC (version 0.11.5)