##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1807132
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.364471992084695	31.0	31.0	34.0	30.0	34.0
2	31.59352609549275	31.0	31.0	34.0	30.0	34.0
3	31.707480693164637	31.0	31.0	34.0	30.0	34.0
4	35.417265036533024	37.0	35.0	37.0	33.0	37.0
5	35.27634948636845	37.0	35.0	37.0	33.0	37.0
6	35.3281160424363	37.0	35.0	37.0	33.0	37.0
7	35.17334040900167	37.0	35.0	37.0	32.0	37.0
8	35.19912601846462	37.0	35.0	37.0	32.0	37.0
9	36.75176080109256	39.0	35.0	39.0	32.0	39.0
10	36.593313604097546	39.0	35.0	39.0	32.0	39.0
11	36.63455574910964	39.0	35.0	39.0	32.0	39.0
12	36.49089441169765	38.0	35.0	39.0	32.0	39.0
13	36.49802283397118	38.0	35.0	39.0	32.0	39.0
14	37.47239659305463	39.0	36.0	41.0	32.0	41.0
15	37.493471976590534	39.0	36.0	41.0	32.0	41.0
16	37.36100240602236	39.0	36.0	41.0	32.0	41.0
17	37.41252548236653	39.0	36.0	41.0	32.0	41.0
18	37.27539327509003	39.0	36.0	40.0	32.0	41.0
19	37.35672712341987	39.0	36.0	41.0	32.0	41.0
20	37.26165603840782	39.0	36.0	40.0	31.0	41.0
21	37.10736349087947	39.0	36.0	40.0	31.0	41.0
22	37.020443996343374	39.0	36.0	40.0	31.0	41.0
23	36.83014522458791	39.0	36.0	40.0	30.0	41.0
24	37.02828736362369	39.0	36.0	40.0	31.0	41.0
25	36.99329600715388	39.0	36.0	40.0	31.0	41.0
26	36.82497072709686	39.0	36.0	40.0	30.0	41.0
27	36.769139719732706	39.0	35.0	40.0	30.0	41.0
28	36.5905927181855	39.0	35.0	40.0	30.0	41.0
29	36.422827994855936	39.0	35.0	40.0	30.0	41.0
30	36.25899768251572	38.0	35.0	40.0	30.0	41.0
31	36.024308683593674	38.0	35.0	40.0	29.0	41.0
32	35.88024947817868	38.0	35.0	40.0	29.0	41.0
33	35.684550990187766	38.0	34.0	40.0	27.0	41.0
34	35.582145631863085	38.0	34.0	40.0	27.0	41.0
35	35.36295356398979	38.0	34.0	40.0	26.0	41.0
36	35.23688529670218	38.0	34.0	40.0	26.0	41.0
37	35.06887820037496	38.0	34.0	40.0	25.0	41.0
38	35.021546848819014	38.0	33.0	40.0	25.0	41.0
39	34.94857542227131	38.0	33.0	40.0	24.0	41.0
40	34.861318376300126	38.0	33.0	40.0	24.0	41.0
41	34.66818361912688	38.0	33.0	40.0	24.0	41.0
42	34.478808963595355	38.0	33.0	40.0	23.0	41.0
43	34.15748600544952	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	9.0
15	7.0
16	38.0
17	64.0
18	159.0
19	368.0
20	859.0
21	1597.0
22	2954.0
23	5048.0
24	8032.0
25	12109.0
26	17491.0
27	24340.0
28	33003.0
29	42826.0
30	54114.0
31	66998.0
32	82733.0
33	101356.0
34	123830.0
35	150757.0
36	188883.0
37	247513.0
38	323361.0
39	318680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.271749933043075	21.47778911557097	13.202798688751017	24.047662262634937
2	19.0800118641029	23.399176153153174	34.601069540022536	22.919742442721393
3	19.33461418424332	25.10602435239927	32.19405112631507	23.36531033704234
4	13.780509669465207	16.636084137738692	36.93576340853906	32.647642784257044
5	12.548723612884945	38.71554485228528	35.311476970138315	13.424254564691458
6	32.19045426676081	38.183375647158044	14.110867385448323	15.515302700632827
7	26.374166358627924	32.54798210645376	22.103365996507172	18.97448553841114
8	26.894548931677377	34.56089538561655	19.346234807418604	19.19832087528747
9	25.06679091510747	14.237864195863944	21.204538462049257	39.490806426979326
10	16.184041896220087	27.50706644561659	34.03951675915207	22.26937489901125
11	33.75685893448846	22.72230252134321	23.253973699762938	20.26686484440539
12	20.944402511825366	27.140408116285915	30.95905556428639	20.956133807602324
13	30.727639154195707	21.161154802194858	25.798558157345454	22.31264788626398
14	20.702250859372754	22.66049187331086	27.473532647310765	29.16372462000562
15	23.175838842984355	29.56900768731891	24.87521664161777	22.379936828078968
16	22.424869904356736	28.301585053001112	26.031966674266183	23.24157836837597
17	22.120409577164256	27.05956178076643	26.872137729839324	23.947890912229987
18	22.765962862701784	26.22470301007342	28.860260346228166	22.149073780996627
19	23.037940781304297	25.541576376269138	29.528612187709584	21.89187065471698
20	23.546868740080967	25.386745406533667	29.329954867713038	21.736430985672325
21	22.679084870391318	25.979729206278236	29.62318192583608	21.71800399749437
22	21.94156265286653	26.352806546505736	29.250657948616926	22.45497285201081
23	22.539692728588726	26.463755829679293	29.855207035235942	21.14134440649604
24	22.595914410236773	26.713211873842084	27.893811852150257	22.79706186377088
25	22.55900509758003	26.384237565379838	28.993122804532263	22.063634532507866
26	22.29671103162359	27.058123036944725	29.010332394091854	21.63483353733983
27	21.71385377493177	27.09326158797476	28.535436260328524	22.65744837676495
28	21.883459536990106	26.797987086720838	28.82395973288061	22.49459364340845
29	22.349723207823224	26.84092805616856	28.364447090749316	22.444901645258895
30	22.124172445621017	26.928138066284035	29.581347682405045	21.3663418056899
31	22.467091501893606	26.802081972982606	29.464754096546354	21.266072428577438
32	21.955120046571032	26.605029405710262	28.752243886998848	22.687606660719858
33	21.898732356020478	26.61891881721977	29.20788298807171	22.274465838688045
34	21.463789031459793	26.850888590318807	29.644154383852424	22.04116799436898
35	21.134372032590868	28.341593198504594	28.84056062313102	21.683474145773523
36	22.864793495992544	26.739883970844406	28.537649712361908	21.857672820801135
37	21.8317754320105	27.053142769869602	28.702883906654304	22.412197891465592
38	21.77942729142088	26.09709750034862	29.808890551437305	22.314584656793198
39	21.342104505924304	25.995500052016123	30.354119123561535	22.30827631849804
40	20.60696174933541	26.53934521661948	30.141793737258816	22.71189929678629
41	21.44956760214528	25.360073309531344	30.369723960396914	22.82063512792646
42	20.48643928611745	26.927806048479024	29.959681971211843	22.62607269419168
43	20.86012532565413	26.311525666083053	29.5985019356638	23.229847072599014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	186.5
2	275.0
3	618.5
4	962.0
5	962.0
6	1571.0
7	2180.0
8	2448.0
9	2716.0
10	4253.0
11	5790.0
12	5790.0
13	10965.0
14	16140.0
15	25231.0
16	34322.0
17	31874.5
18	29427.0
19	29427.0
20	31705.0
21	33983.0
22	28797.5
23	23612.0
24	27228.0
25	30844.0
26	30844.0
27	34886.5
28	38929.0
29	43196.5
30	47464.0
31	53790.0
32	60116.0
33	60116.0
34	67818.0
35	75520.0
36	83143.5
37	90767.0
38	95619.5
39	100472.0
40	100472.0
41	103160.0
42	105848.0
43	111435.5
44	117023.0
45	119862.0
46	122701.0
47	122701.0
48	143948.5
49	165196.0
50	158761.0
51	152326.0
52	141811.0
53	131296.0
54	131296.0
55	119677.0
56	108058.0
57	94439.5
58	80821.0
59	72994.5
60	65168.0
61	65168.0
62	57635.5
63	50103.0
64	43547.0
65	36991.0
66	31564.0
67	26137.0
68	26137.0
69	22508.0
70	18879.0
71	15861.5
72	12844.0
73	10628.0
74	8412.0
75	8412.0
76	6746.0
77	5080.0
78	4087.5
79	3095.0
80	2388.5
81	1682.0
82	1682.0
83	1289.5
84	897.0
85	678.0
86	459.0
87	355.0
88	251.0
89	251.0
90	203.5
91	156.0
92	106.5
93	57.0
94	41.5
95	26.0
96	26.0
97	16.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1807132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.95041212535422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.40687052697722	47.500556505974124
2	10.054732746263076	11.452423474199534
3	2.7583863106538953	4.71273711578024
4	1.1806692025279628	2.689583906707231
5	0.5989584177264304	1.705546436773514
6	0.35854340774024906	1.2251516901381658
7	0.2508296834608103	0.9999397692455706
8	0.17365906882315216	0.7911964431027013
9	0.13656764220631118	0.6999825155973607
>10	0.9142494210566562	10.174906691324558
>50	0.0916232316417347	3.60593039192542
>100	0.06644698539893515	7.359261819132552
>500	0.00554495706722157	2.0333396728703956
>1k	0.0025292786622414178	2.3971602175747684
>5k	9.727994854774685E-5	0.3461475199197382
>10k+	2.918398456432405E-4	2.306135829734158
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16654	0.9215707541009732	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13614	0.7533483995635072	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11358	0.6285097048804404	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6248	0.34574120761516036	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3456	0.1912422556846982	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3269	0.18089436742861065	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3222	0.1782935612893801	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2814	0.15571635054882543	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2714	0.15018272046535616	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2579	0.14271231985267263	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.533630083469276E-5	0.0
3	0.0	0.0	0.0	5.533630083469276E-5	0.0
4	0.0	0.0	0.0	5.533630083469276E-5	0.0
5	0.0	0.0	0.0	5.533630083469276E-5	0.0
6	0.0	0.0	0.0	5.533630083469276E-5	0.0
7	0.0	0.0	0.0	1.1067260166938552E-4	0.0
8	0.0	0.0	0.0	1.660089025040783E-4	0.0
9	0.0	0.0	0.0	3.320178050081566E-4	0.0
10	1.1067260166938552E-4	0.0	0.0	3.873541058428493E-4	0.0
11	1.1067260166938552E-4	0.0	0.0	3.873541058428493E-4	0.0
12	1.1067260166938552E-4	0.0	0.0	6.086993091816204E-4	0.0
13	1.1067260166938552E-4	0.0	0.0	6.086993091816204E-4	0.0
14	1.1067260166938552E-4	0.0	0.0	6.086993091816204E-4	0.0
15	1.1067260166938552E-4	0.0	0.0	9.40717114189777E-4	0.0
16	1.660089025040783E-4	0.0	0.0	0.0012727349191979335	0.0
17	1.660089025040783E-4	0.0	0.0	0.001881434228379554	0.0
18	1.660089025040783E-4	0.0	0.0	0.002047443130883632	0.0
19	2.2134520333877105E-4	0.0	0.0	0.0023241246350570958	0.0
20	2.2134520333877105E-4	0.0	0.0	0.003984213660097879	0.0
21	2.766815041734638E-4	0.0	0.0	0.006086993091816204	0.0
22	2.766815041734638E-4	0.0	0.0	0.011177932768607938	0.0
23	2.766815041734638E-4	0.0	0.0	0.013336048501160956	0.0
24	2.766815041734638E-4	0.0	0.0	0.017375598462093526	0.0
25	2.766815041734638E-4	0.0	0.0	0.021138466918852634	0.0
26	2.766815041734638E-4	0.0	0.0	0.027391468913172918	0.0
27	2.766815041734638E-4	0.0	0.0	0.07143916437758835	0.0
28	2.766815041734638E-4	0.0	0.0	0.16362944156818648	0.0
29	2.766815041734638E-4	0.0	0.0	0.2769028493768026	0.0
30	2.766815041734638E-4	0.0	0.0	0.42514879931294447	0.0
31	2.766815041734638E-4	0.0	0.0	0.6010075633655981	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4940	0.0	21.758099	1
CTAATAC	470	0.0	20.468086	3
CTCTAAT	415	0.0	18.72289	1
TAATACT	530	0.0	18.150944	4
TTAACGG	290	0.0	17.862068	35
GTATTGG	565	0.0	16.371681	1
TCTAATA	465	0.0	16.311827	2
GTCTAGA	115	1.243081E-6	16.086956	1
GTATTAG	515	0.0	15.805824	1
ATGCGTA	95	7.06325E-5	15.578948	7
ATACTGG	525	0.0	15.504762	6
CCGTTTA	275	0.0	15.472727	27
ATGCTAG	450	0.0	15.211111	15
AATACTG	625	0.0	15.096	5
ACCGTTA	320	0.0	15.03125	8
CGCTAGA	75	0.004105615	14.8	1
TACTGGT	550	0.0	14.799999	7
ATTAGAG	445	0.0	14.550561	3
GACCGTT	325	0.0	14.230769	7
GATGCTA	495	0.0	14.202021	14
>>END_MODULE