FastQCFastQC Report
Wed 25 May 2016
SRR2088489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949826
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT333241.1296937514280503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT282900.9590396179300067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT228970.7762152750704618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120610.4088715741199651No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77960.2642867748809591No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG73040.24760782500391548No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA72490.2457433082493679No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68300.23153908061017836No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA64510.2186908651561143No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG57770.19584205983674968No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39030.13231288896362023No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA37740.1279397496665905No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35490.12031218112525958No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34790.11793915980128997No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33470.11346431959037584No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.10566724952590424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91000.023.9076921
TATACCG1850.020.05
GTATTGG11150.017.9192831
TCGGGTA2850.016.87719325
TAGCGAA3950.015.9240510
TTGGACC17350.015.7809794
GTCGGGT3000.015.41666724
GTATCAA142800.015.2871152
TGGACCC17250.015.2289855
TATACAG4250.014.85
AATCGGC1252.9623661E-614.79999925
TTGTCGG4500.014.38888922
TTCGCTA4250.014.36470626
AGTTCCG3750.014.30666632
TCGCTAC4400.014.29545427
TGTCGGG4550.014.23076923
GGACCCT17700.014.214696
ATTGGAC14200.014.2007043
TTAACGG4050.014.16049435
TAGGAGT2800.013.8750014
Produced by FastQC (version 0.11.5)