FastQCFastQC Report
Wed 25 May 2016
SRR2088488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022189
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT415291.3741364289261857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT360571.1930756150591508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT292060.9663856231360778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151780.5022187560076488No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG125460.4151295633727738No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG101860.33704046967281004No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA97400.32228295450747785No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA91230.3018672889088009No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA89390.2957789866881257No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG79200.26206170428123454No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA54840.1814578770553397No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC48100.1591561613122144No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48010.15885836392098576No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA45610.1509171001548877No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.14688029107378792No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG42160.13950153349112185No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA39450.13053452315523614No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37820.1251410815140946No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC36430.12054176624956281No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36400.1204425004524866No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG36070.11935057668464812No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35770.11835791871388586No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG34870.1153799448015991No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30590.1012180244187243No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30530.10101949282457186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101500.026.1733971
GTATTGG12250.020.3877541
ATTGGAC14350.017.9198593
TTGGACC17800.017.668544
ACCGTTA3600.017.4722238
CCGTTTA3350.016.56716327
GTATTAG6450.016.3488371
TGGACCC18700.016.323535
GTATCAA168500.015.7661712
TAGGTCG1302.592842E-715.65384621
CGTTTAC3500.015.32857128
GGACCCT19850.015.2846356
TTAACGG4600.015.28260935
AATACTG6300.015.2698425
TAACCCG855.367744E-415.2352945
TATTGGA16550.015.2024172
CATTGCG1406.0036837E-714.53571429
CGCCGTT3700.014.525
TGTTACG1956.7484507E-1014.23076816
ATTAGAG6150.014.1382113
Produced by FastQC (version 0.11.5)