FastQCFastQC Report
Wed 25 May 2016
SRR2088487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551186
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT319991.2542793822167415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT272261.0671899265674867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT221640.8687724062455657No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119210.46727286838356746No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG75150.29456887894492995No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG67680.2652883796007034No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63050.24713995765106894No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA62830.24627761362754422No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA59650.23381282274205017No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG52800.2069625656459388No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35710.139974114000312No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34670.13589757861637686No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA33570.13158585849875312No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC33560.1315466610431384No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32980.12927320861748223No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.12453031648809612No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28190.11049762737801164No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.11018404773309354No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG27250.1068130665502241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86550.023.2772961
GTATTAG4750.015.1894731
TAATACT5900.015.0508464
GTATCAA134350.014.9955332
GTATTGG11600.014.9913791
CGAAACG750.0041062314.824
TCTAATA5650.014.407082
CGAATTA908.27889E-414.38888915
CTCTAAT5400.014.0462971
CTAATAC5800.014.0344833
TAGTACT4400.013.8754
TGCTAGA4700.013.77659516
ACGTTCG2151.9826984E-1013.76744222
CGTACTG1356.574017E-613.7037045
ATGCTAG4950.013.45454615
TACTGGT6200.013.427427
GGACCCT16850.013.3946586
GGTAAAC4850.013.35051535
TTGGACC17050.013.3460424
GTTATCG1254.8489084E-513.3211
Produced by FastQC (version 0.11.5)