##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2551186
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.355674576451893	31.0	31.0	34.0	30.0	34.0
2	31.573609293873517	31.0	31.0	34.0	30.0	34.0
3	31.680764554211258	31.0	31.0	34.0	30.0	34.0
4	35.38482885998904	37.0	35.0	37.0	33.0	37.0
5	35.25922806098811	37.0	35.0	37.0	33.0	37.0
6	35.316396766053124	37.0	35.0	37.0	33.0	37.0
7	35.149405021821224	37.0	35.0	37.0	32.0	37.0
8	35.20027665564173	37.0	35.0	37.0	32.0	37.0
9	36.73368503903674	39.0	35.0	39.0	32.0	39.0
10	36.587273134926264	39.0	35.0	39.0	32.0	39.0
11	36.622280382535806	39.0	35.0	39.0	32.0	39.0
12	36.47838573902491	38.0	35.0	39.0	32.0	39.0
13	36.496273497894705	38.0	35.0	39.0	32.0	39.0
14	37.434588070019196	39.0	36.0	41.0	32.0	41.0
15	37.45842325882942	39.0	36.0	41.0	32.0	41.0
16	37.32017932051995	39.0	36.0	41.0	32.0	41.0
17	37.411277735139656	39.0	36.0	41.0	32.0	41.0
18	37.285988947885414	39.0	36.0	40.0	32.0	41.0
19	37.37750089566186	39.0	36.0	41.0	32.0	41.0
20	37.286684702722575	39.0	36.0	40.0	31.0	41.0
21	37.13716796815285	39.0	36.0	40.0	31.0	41.0
22	37.052324683500146	39.0	36.0	40.0	31.0	41.0
23	36.85853207096621	39.0	36.0	40.0	30.0	41.0
24	37.04116124814106	39.0	36.0	40.0	31.0	41.0
25	37.00466567314182	39.0	36.0	40.0	31.0	41.0
26	36.82352795915312	39.0	36.0	40.0	30.0	41.0
27	36.75688758091335	39.0	35.0	40.0	30.0	41.0
28	36.55790287340868	39.0	35.0	40.0	30.0	41.0
29	36.380747620910434	38.0	35.0	40.0	30.0	41.0
30	36.197495596165865	38.0	35.0	40.0	30.0	41.0
31	35.91561963729811	38.0	35.0	40.0	29.0	41.0
32	35.769595004049094	38.0	34.0	40.0	28.0	41.0
33	35.55465105249088	38.0	34.0	40.0	27.0	41.0
34	35.43255568194557	38.0	34.0	40.0	27.0	41.0
35	35.195429890254964	38.0	34.0	40.0	26.0	41.0
36	35.03725404576538	38.0	34.0	40.0	25.0	41.0
37	34.85847249083368	38.0	33.0	40.0	25.0	41.0
38	34.775649443043356	38.0	33.0	40.0	24.0	41.0
39	34.66382184599633	38.0	33.0	40.0	24.0	41.0
40	34.537444545399666	38.0	33.0	40.0	23.0	41.0
41	34.30123323034855	38.0	33.0	40.0	22.0	41.0
42	34.06822944309039	38.0	33.0	40.0	21.0	41.0
43	33.736654246299565	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	3.0
14	3.0
15	14.0
16	32.0
17	109.0
18	219.0
19	548.0
20	1163.0
21	2346.0
22	4342.0
23	7310.0
24	11967.0
25	18138.0
26	26263.0
27	36210.0
28	48658.0
29	63331.0
30	78820.0
31	97326.0
32	118911.0
33	144950.0
34	176682.0
35	213591.0
36	267571.0
37	353144.0
38	441791.0
39	437739.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.530880147507865	21.39514719820507	13.83176295260322	23.242209701683844
2	19.183705147331477	23.824683892119193	34.517632191459185	22.47397876909014
3	19.888749781474186	24.863024491354217	32.57073376852962	22.67749195864198
4	13.951001612583324	16.437335419683237	36.18038825863735	33.43127470909608
5	12.450327024372195	38.84401215748283	34.922267525770366	13.783393292374605
6	32.36443755962913	37.291792915138295	13.599753212819449	16.74401631241313
7	25.97364519874286	32.93272227113194	21.573025251784856	19.52060727834035
8	27.28777909568334	34.23317625606286	18.952165777015082	19.526878871238708
9	24.629682038079544	14.32235830707757	21.695360510758526	39.35259914408436
10	16.473240289026357	27.704056074312106	33.36103286863443	22.461670768027105
11	33.24708586516232	22.41776961773857	23.501696857853563	20.833447659245543
12	21.05671636642722	27.31298305964363	30.87364073023292	20.756659843696227
13	31.625487126379653	20.952255147213883	26.264255134670698	21.158002591735762
14	20.55102999154119	23.001223744564292	27.75379764548724	28.693948618407283
15	22.70218635567928	29.526032206197435	25.604091587206888	22.167689850916396
16	21.82416334990863	28.42528925762371	26.577795582133174	23.17275181033449
17	21.734205189272753	26.80286737227313	27.141337401506593	24.321590036947523
18	22.95963524415703	25.64615045708153	29.471194965792378	21.92301933296906
19	22.756004462238348	24.87713557537553	30.6425717293839	21.72428823300222
20	23.665307037589574	24.62752617802073	30.456971777048008	21.25019500734168
21	22.51999658198187	25.40238148061333	30.987430943882572	21.090190993522228
22	21.258073695920253	25.878395381598988	30.48621307893662	22.37731784354414
23	22.228916276586656	26.043259879914675	31.386853016596987	20.340970826901682
24	22.166866704348486	26.139842410549445	28.970016298302042	22.72327458680002
25	22.07302799560675	26.304471724131446	30.13186808017918	21.49063220008263
26	21.90310702551676	26.942292721894834	30.005377890910346	21.149222361678056
27	20.907570047813056	27.027312003123257	29.652757580199957	22.412360368863737
28	21.33035380407387	26.55412031894186	29.92431755269902	22.191208324285256
29	21.64691245561868	26.53107221504038	29.29547277227141	22.526542557069533
30	21.553073746876944	26.696995044657662	30.892141929283085	20.85778927918231
31	22.13460719837754	26.48909174007697	30.71493023244875	20.661370829096743
32	21.814559973283014	26.30831307478169	29.63527551499577	22.241851436939523
33	21.491651333928612	26.360210506015637	30.261533263352806	21.886604896702945
34	20.725693853760564	27.021197200047354	30.7155965891942	21.537512356997883
35	20.426342885230632	28.894678788610474	29.62010609967286	21.058872226486034
36	22.504552784469656	26.584380754676452	29.540966436786654	21.370100024067238
37	21.454805725650736	27.33853980070446	29.457319066504756	21.74933540714005
38	21.356694494246987	25.847115812018412	30.82064577024176	21.97554392349284
39	20.85022417024866	25.757824008127983	31.529296570301028	21.862655251322327
40	19.92880958111247	26.323560885015834	31.11568501865407	22.63194451521763
41	21.260935110180128	25.27577369897765	31.091069016528	22.37222217431422
42	19.997875497905678	27.06278570045461	30.59580916483549	22.343529636804217
43	20.306986632883685	26.848571605519943	29.88010282276557	22.964338938830803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	174.0
1	324.0
2	474.0
3	1158.5
4	1843.0
5	1843.0
6	2998.0
7	4153.0
8	4619.5
9	5086.0
10	7864.5
11	10643.0
12	10643.0
13	20479.5
14	30316.0
15	47796.5
16	65277.0
17	59610.5
18	53944.0
19	53944.0
20	57247.0
21	60550.0
22	48555.0
23	36560.0
24	40631.0
25	44702.0
26	44702.0
27	48889.5
28	53077.0
29	58019.5
30	62962.0
31	70202.5
32	77443.0
33	77443.0
34	86201.0
35	94959.0
36	103553.0
37	112147.0
38	119101.5
39	126056.0
40	126056.0
41	130274.0
42	134492.0
43	142605.0
44	150718.0
45	159621.5
46	168525.0
47	168525.0
48	215940.5
49	263356.0
50	248138.0
51	232920.0
52	213352.0
53	193784.0
54	193784.0
55	173828.0
56	153872.0
57	131677.0
58	109482.0
59	97356.0
60	85230.0
61	85230.0
62	75237.0
63	65244.0
64	56959.5
65	48675.0
66	42056.0
67	35437.0
68	35437.0
69	30153.5
70	24870.0
71	21125.0
72	17380.0
73	14430.5
74	11481.0
75	11481.0
76	9257.0
77	7033.0
78	5423.0
79	3813.0
80	2990.5
81	2168.0
82	2168.0
83	1650.0
84	1132.0
85	867.0
86	602.0
87	454.0
88	306.0
89	306.0
90	244.5
91	183.0
92	129.5
93	76.0
94	50.5
95	25.0
96	25.0
97	18.0
98	11.0
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2551186.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.22605018544994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4809918768158	40.4224233697974
2	11.17808426233978	11.228620422749321
3	3.4724163177024496	5.232172687130956
4	1.4363444421216711	2.885676321343806
5	0.8005315771252199	2.010376958386434
6	0.47717625824309523	1.4380007216293753
7	0.34234935017542134	1.2036398950006821
8	0.23237607702655275	0.9337066005306895
9	0.18649259526690398	0.8430107804181292
>10	1.1882270629811806	11.420974723339645
>50	0.11475826507084835	3.965703560265605
>100	0.07937844084144913	7.610105704766077
>500	0.006023435397862342	2.004853272548797
>1k	0.00406777455440017	3.6370011510236484
>5k	4.6935860243078886E-4	1.4975794645278862
>10k+	3.129057349538592E-4	3.6661543665415333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31999	1.2542793822167415	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27226	1.0671899265674867	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22164	0.8687724062455657	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11921	0.46727286838356746	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7515	0.29456887894492995	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6768	0.2652883796007034	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6305	0.24713995765106894	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6283	0.24627761362754422	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5965	0.23381282274205017	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5280	0.2069625656459388	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3571	0.139974114000312	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3467	0.13589757861637686	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3357	0.13158585849875312	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3356	0.1315466610431384	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3298	0.12927320861748223	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3177	0.12453031648809612	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2819	0.11049762737801164	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2811	0.11018404773309354	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2725	0.1068130665502241	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.919745561476113E-5	0.0	0.0	0.0	0.0
6	3.919745561476113E-5	0.0	0.0	0.0	0.0
7	3.919745561476113E-5	0.0	0.0	0.0	0.0
8	7.839491122952227E-5	0.0	0.0	3.919745561476113E-5	0.0
9	1.5678982245904453E-4	0.0	0.0	7.839491122952227E-5	0.0
10	2.351847336885668E-4	0.0	0.0	1.9598727807380566E-4	0.0
11	3.1357964491808907E-4	0.0	0.0	2.7438218930332794E-4	0.0
12	3.1357964491808907E-4	0.0	0.0	4.703694673771336E-4	0.0
13	3.1357964491808907E-4	0.0	0.0	5.487643786066559E-4	0.0
14	3.919745561476113E-4	0.0	0.0	6.663567454509393E-4	0.0
15	3.919745561476113E-4	0.0	0.0	9.407389347542673E-4	0.0
16	3.919745561476113E-4	0.0	0.0	0.001489503313360923	0.0
17	3.919745561476113E-4	0.0	0.0	0.0025086371593447125	0.0
18	3.919745561476113E-4	0.0	0.0	0.0032141913604104132	0.0
19	4.311720117623725E-4	0.0	0.0	0.004350917573238486	0.0
20	4.311720117623725E-4	0.0	0.0	0.0076827013004931825	0.0
21	4.703694673771336E-4	0.0	0.0	0.012621580707953085	0.0
22	4.703694673771336E-4	0.0	0.0	0.01897156851754439	0.0
23	4.703694673771336E-4	0.0	0.0	0.023008906445864785	0.0
24	4.703694673771336E-4	0.0	0.0	0.02869253751000515	0.0
25	4.703694673771336E-4	0.0	0.0	0.03347462709500601	0.0
26	4.703694673771336E-4	0.0	0.0	0.04221565969709774	0.0
27	4.703694673771336E-4	0.0	0.0	0.0947010527652629	0.0
28	4.703694673771336E-4	0.0	0.0	0.20633540635610262	0.0
29	4.703694673771336E-4	0.0	0.0	0.3412530485821104	0.0
30	4.703694673771336E-4	0.0	0.0	0.5246579434035777	0.0
31	5.095669229918948E-4	0.0	0.0	0.7252313237843105	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8655	0.0	23.277296	1
GTATTAG	475	0.0	15.189473	1
TAATACT	590	0.0	15.050846	4
GTATCAA	13435	0.0	14.995533	2
GTATTGG	1160	0.0	14.991379	1
CGAAACG	75	0.00410623	14.8	24
TCTAATA	565	0.0	14.40708	2
CGAATTA	90	8.27889E-4	14.388889	15
CTCTAAT	540	0.0	14.046297	1
CTAATAC	580	0.0	14.034483	3
TAGTACT	440	0.0	13.875	4
TGCTAGA	470	0.0	13.776595	16
ACGTTCG	215	1.9826984E-10	13.767442	22
CGTACTG	135	6.574017E-6	13.703704	5
ATGCTAG	495	0.0	13.454546	15
TACTGGT	620	0.0	13.42742	7
GGACCCT	1685	0.0	13.394658	6
GGTAAAC	485	0.0	13.350515	35
TTGGACC	1705	0.0	13.346042	4
GTTATCG	125	4.8489084E-5	13.32	11
>>END_MODULE