FastQCFastQC Report
Wed 25 May 2016
SRR2088486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2865160
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT418461.4605118038783176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT355121.2394421253961385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT289471.0103100699437377No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148210.5172835024920075No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG104730.3655293247148501No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG94030.32818411537226544No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA92680.3234723366234346No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82950.2895126275670469No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA81700.28514986946627763No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG74870.2613117592036745No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA51270.1789428862611512No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA47700.16648284912535424No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC46660.16285303438551424No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44100.15391810579513884No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA42860.14959024975917573No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.13056862443982187No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA36600.1277415571905234No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33980.11859721621131107No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG33930.11842270588728028No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC33470.1168172109061972No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG33180.11580505102681873No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC32380.11301288584232644No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG31760.11084895782434488No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA31020.1082662050286895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114350.023.2807181
GTATTGG13150.019.1330811
ATTGGAC15700.017.0859873
GTCGTAC650.001580712717.0769231
TTGGACC20950.016.4248224
TAACGGC3850.016.33766436
TTAACGG4000.015.72535
GGACCCT20350.015.5454556
TGGACCC20550.015.4841855
AACGGCC4250.015.23529437
GTATTAG6250.015.0961
TATACTG2950.015.0508475
ACCGTTA3700.015.08
TAATACT7400.014.754
GTATCAA181000.014.7080112
GGTAAAC5600.014.53571535
TATTGGA17650.014.464592
TCGGGTA2950.014.42372925
TAGATGT5900.014.4237294
TAGGTCG1304.4492535E-614.23076921
Produced by FastQC (version 0.11.5)