##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2865160
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39044451269737	31.0	31.0	34.0	30.0	34.0
2	31.604520166413046	31.0	31.0	34.0	30.0	34.0
3	31.70482939870723	31.0	31.0	34.0	30.0	34.0
4	35.39591541135574	37.0	35.0	37.0	33.0	37.0
5	35.29017576679836	37.0	35.0	37.0	33.0	37.0
6	35.36174454480727	37.0	35.0	37.0	33.0	37.0
7	35.19640159711849	37.0	35.0	37.0	32.0	37.0
8	35.25876146532829	37.0	35.0	37.0	32.0	37.0
9	36.785539725530164	39.0	37.0	39.0	32.0	39.0
10	36.65002896871379	39.0	35.0	39.0	32.0	39.0
11	36.688062795795	39.0	35.0	39.0	32.0	39.0
12	36.53332274637368	38.0	35.0	39.0	32.0	39.0
13	36.56249773136579	38.0	35.0	39.0	32.0	39.0
14	37.491348825196496	39.0	36.0	41.0	32.0	41.0
15	37.51666748104818	39.0	36.0	41.0	32.0	41.0
16	37.37710773569364	39.0	36.0	41.0	32.0	41.0
17	37.48508006533667	39.0	36.0	41.0	32.0	41.0
18	37.362406636976644	39.0	36.0	41.0	32.0	41.0
19	37.46202201622248	39.0	36.0	41.0	32.0	41.0
20	37.386848552960394	39.0	36.0	41.0	32.0	41.0
21	37.24344574125005	39.0	36.0	41.0	31.0	41.0
22	37.148695709838194	39.0	36.0	40.0	31.0	41.0
23	36.96485187563696	39.0	36.0	40.0	31.0	41.0
24	37.1449761967918	39.0	36.0	41.0	31.0	41.0
25	37.098614737047846	39.0	36.0	41.0	31.0	41.0
26	36.93597565231959	39.0	36.0	40.0	31.0	41.0
27	36.86902720964972	39.0	35.0	40.0	31.0	41.0
28	36.67957880188192	39.0	35.0	40.0	30.0	41.0
29	36.49577440701392	39.0	35.0	40.0	30.0	41.0
30	36.302467575981794	38.0	35.0	40.0	30.0	41.0
31	36.01552862667356	38.0	35.0	40.0	29.0	41.0
32	35.875589844895224	38.0	35.0	40.0	29.0	41.0
33	35.65181874659705	38.0	34.0	40.0	28.0	41.0
34	35.54281366485641	38.0	34.0	40.0	27.0	41.0
35	35.28082585265744	38.0	34.0	40.0	26.0	41.0
36	35.108066565217996	38.0	34.0	40.0	25.0	41.0
37	34.9294922447612	38.0	33.0	40.0	25.0	41.0
38	34.83277478395622	38.0	33.0	40.0	24.0	41.0
39	34.713119337139986	38.0	33.0	40.0	24.0	41.0
40	34.55567996202655	38.0	33.0	40.0	23.0	41.0
41	34.31660500635218	38.0	33.0	40.0	22.0	41.0
42	34.06595478088484	38.0	33.0	40.0	21.0	41.0
43	33.720865152382416	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	0.0
13	3.0
14	10.0
15	16.0
16	44.0
17	95.0
18	215.0
19	592.0
20	1183.0
21	2391.0
22	4443.0
23	7603.0
24	12556.0
25	18920.0
26	27423.0
27	38830.0
28	52574.0
29	68646.0
30	86508.0
31	106581.0
32	131429.0
33	160640.0
34	196548.0
35	239130.0
36	304802.0
37	406361.0
38	504740.0
39	492872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57589803012746	21.500055843303688	14.157324547320218	22.766721579248628
2	19.170971254659424	24.038622624914492	34.74235295760097	22.048053162825113
3	19.78367700233146	24.745424339303913	33.28924039146156	22.18165826690307
4	13.6467771433358	16.363902888494884	36.4093453768725	33.57997459129682
5	12.092308980999315	39.141095087185356	34.99019251978947	13.776403412025855
6	31.845307068366164	37.54963073615435	13.162092169372741	17.442970026106742
7	25.5983609990367	33.460225606946906	21.229320526602354	19.712092867414036
8	27.545233075988772	34.03481829985061	18.66848622764523	19.75146239651538
9	24.33218389199905	14.310265395300787	22.07457873207779	39.28297198062237
10	16.621235812310655	27.642191012020273	33.07595387343115	22.66061930223792
11	32.77635454913513	22.48792388557707	23.762337879908976	20.973383685378828
12	21.252914322411314	27.67053148864287	30.723484901366767	20.353069287579054
13	32.10735177093077	20.811228692289436	26.34233341244468	20.739086124335117
14	20.334990018009467	23.353460190704883	27.945804073769004	28.36574571751665
15	22.45082299068813	29.806188834131426	25.967415432296974	21.77557274288347
16	21.549128146421143	28.49840846584484	26.828309762805567	23.12415362492845
17	21.650797861201468	26.727512599645397	27.04016529617892	24.581524242974215
18	23.07071856371023	25.40528277652906	29.86698823102375	21.657010428736964
19	22.658036549442265	24.597614094849853	31.145206550419523	21.59914280528836
20	23.735184073489787	24.140327241759625	31.029192087003864	21.095296597746724
21	22.425449189574056	25.185923299222384	31.70786273715953	20.680764774044032
22	20.931850228259506	25.630052073880687	31.21982716497508	22.218270532884727
23	22.109550601013556	25.89363246729677	32.21669993996844	19.78011699172123
24	21.99817811221712	25.901345823618925	29.35242708958662	22.748048974577337
25	21.790057099778025	26.133653966968684	30.82117578075919	21.2551131524941
26	21.6277275963646	26.996118890393557	30.635147775342393	20.741005737899453
27	20.305218556729816	27.06941322648648	30.222919487916904	22.4024487288668
28	21.03194935012355	26.545603037875722	30.328812352538776	22.093635259461948
29	21.42564464113697	26.547662259699283	29.576393639447712	22.450299459716035
30	21.249249605606668	26.625354255957784	31.74091499253096	20.384481145904594
31	22.025052702117858	26.483756579039213	31.297030532326293	20.194160186516637
32	21.50913038015329	26.25183235840232	30.13887531586369	22.1001619455807
33	21.14185595219813	26.31458627092379	30.85611274763015	21.687445029247932
34	20.261137248879642	27.210103449720087	31.338878108028872	21.1898811933714
35	19.8497117089447	29.544318641890854	30.007050217090843	20.5989194320736
36	22.47378854933058	26.77086096413464	29.897213419145878	20.858137067388906
37	21.255043348364488	27.660654204302727	29.707695207248463	21.376607240084322
38	21.07072554412319	25.908361138644963	31.36830054866046	21.652612768571387
39	20.571521311200772	25.787460386156447	32.055452400564015	21.58556590207877
40	19.31640815870667	26.565985843722515	31.611637744488963	22.50596825308185
41	20.962319730835276	25.352161833894094	31.68482737438747	22.000691060883163
42	19.584176799899485	27.514309846570523	31.010624188526993	21.890889165003003
43	19.985550545170252	27.273276187019224	30.136013346549582	22.60515992126094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	183.0
1	366.0
2	549.0
3	1329.0
4	2109.0
5	2109.0
6	3424.5
7	4740.0
8	5452.0
9	6164.0
10	9467.5
11	12771.0
12	12771.0
13	24895.0
14	37019.0
15	59979.5
16	82940.0
17	74994.0
18	67048.0
19	67048.0
20	70699.5
21	74351.0
22	57712.0
23	41073.0
24	45029.0
25	48985.0
26	48985.0
27	53142.5
28	57300.0
29	62136.5
30	66973.0
31	74025.5
32	81078.0
33	81078.0
34	90802.5
35	100527.0
36	111081.0
37	121635.0
38	129572.0
39	137509.0
40	137509.0
41	142228.5
42	146948.0
43	157930.0
44	168912.0
45	180767.5
46	192623.0
47	192623.0
48	257585.5
49	322548.0
50	300703.0
51	278858.0
52	252484.0
53	226110.0
54	226110.0
55	199305.0
56	172500.0
57	144419.5
58	116339.0
59	101178.5
60	86018.0
61	86018.0
62	75415.0
63	64812.0
64	56405.0
65	47998.0
66	41198.0
67	34398.0
68	34398.0
69	29322.0
70	24246.0
71	20377.5
72	16509.0
73	13346.5
74	10184.0
75	10184.0
76	8110.0
77	6036.0
78	4721.5
79	3407.0
80	2599.5
81	1792.0
82	1792.0
83	1393.0
84	994.0
85	736.0
86	478.0
87	371.0
88	264.0
89	264.0
90	194.0
91	124.0
92	94.0
93	64.0
94	48.0
95	32.0
96	32.0
97	20.5
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2865160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.62825984865885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.8313876612008	35.18107652774428
2	11.917623396244407	10.637255874121031
3	3.8592547691048362	5.166954739733597
4	1.620886775818402	2.89349424865914
5	0.8630175438293614	1.9257485599984037
6	0.5097775608075935	1.3650291269242063
7	0.35840569734979677	1.1196515814796557
8	0.2501379769923645	0.8930578108186473
9	0.2027111031524168	0.8141979407124911
>10	1.3112439361420556	11.566784920655044
>50	0.15350049441985528	4.739544534800781
>100	0.10576496247904288	9.346725457214163
>500	0.0097571361589611	2.9575427248171335
>1k	0.0056654338987505905	5.094835358014155
>5k	4.7211949156254914E-4	1.6768105948634993
>10k+	3.9343290963545765E-4	4.621289999443803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41846	1.4605118038783176	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35512	1.2394421253961385	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28947	1.0103100699437377	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14821	0.5172835024920075	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10473	0.3655293247148501	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9403	0.32818411537226544	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9268	0.3234723366234346	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8295	0.2895126275670469	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8170	0.28514986946627763	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7487	0.2613117592036745	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5127	0.1789428862611512	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4770	0.16648284912535424	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4666	0.16285303438551424	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4410	0.15391810579513884	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4286	0.14959024975917573	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3741	0.13056862443982187	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3660	0.1277415571905234	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3398	0.11859721621131107	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3393	0.11842270588728028	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3347	0.1168172109061972	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3318	0.11580505102681873	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3238	0.11301288584232644	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3176	0.11084895782434488	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3102	0.1082662050286895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.490206480615393E-5	0.0	0.0	0.0	0.0
7	3.490206480615393E-5	0.0	0.0	0.0	0.0
8	2.094123888369236E-4	0.0	0.0	0.0	0.0
9	2.094123888369236E-4	0.0	0.0	2.094123888369236E-4	0.0
10	2.7921651844923145E-4	0.0	0.0	2.7921651844923145E-4	0.0
11	2.7921651844923145E-4	0.0	0.0	3.4902064806153934E-4	0.0
12	3.141185832553854E-4	0.0	0.0	5.23530972092309E-4	0.0
13	3.141185832553854E-4	3.490206480615393E-5	0.0	5.23530972092309E-4	0.0
14	3.141185832553854E-4	3.490206480615393E-5	0.0	6.980412961230787E-4	0.0
15	3.141185832553854E-4	3.490206480615393E-5	0.0	9.074536849600023E-4	0.0
16	3.141185832553854E-4	6.980412961230786E-5	0.0	0.0013611805274400034	0.0
17	3.8392271286769324E-4	6.980412961230786E-5	0.0	0.0022337321475938516	0.0
18	4.537268424800011E-4	6.980412961230786E-5	0.0	0.002966675508523084	0.0
19	4.537268424800011E-4	6.980412961230786E-5	0.0	0.0037694229990646246	0.0
20	4.88628907286155E-4	6.980412961230786E-5	0.0	0.006701196442781555	0.0
21	5.23530972092309E-4	6.980412961230786E-5	0.0	0.011517681386030798	0.0
22	5.23530972092309E-4	6.980412961230786E-5	0.0	0.017904759245556966	0.0
23	5.23530972092309E-4	6.980412961230786E-5	0.0	0.021953398763070824	0.0
24	5.23530972092309E-4	6.980412961230786E-5	0.0	0.027747141520892377	0.0
25	5.23530972092309E-4	6.980412961230786E-5	0.0	0.03231931201049854	0.0
26	5.23530972092309E-4	1.047061944184618E-4	0.0	0.04135894679529241	0.0
27	5.23530972092309E-4	1.047061944184618E-4	0.0	0.09975010121598794	0.0
28	5.23530972092309E-4	2.443144536430775E-4	0.0	0.21946418350109592	0.0
29	5.933351017046168E-4	2.443144536430775E-4	0.0	0.36901953119546554	0.0
30	5.933351017046168E-4	2.443144536430775E-4	0.0	0.5766868167920814	0.0
31	6.282371665107708E-4	2.443144536430775E-4	0.0	0.7885423501654358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11435	0.0	23.280718	1
GTATTGG	1315	0.0	19.133081	1
ATTGGAC	1570	0.0	17.085987	3
GTCGTAC	65	0.0015807127	17.076923	1
TTGGACC	2095	0.0	16.424822	4
TAACGGC	385	0.0	16.337664	36
TTAACGG	400	0.0	15.725	35
GGACCCT	2035	0.0	15.545455	6
TGGACCC	2055	0.0	15.484185	5
AACGGCC	425	0.0	15.235294	37
GTATTAG	625	0.0	15.096	1
TATACTG	295	0.0	15.050847	5
ACCGTTA	370	0.0	15.0	8
TAATACT	740	0.0	14.75	4
GTATCAA	18100	0.0	14.708011	2
GGTAAAC	560	0.0	14.535715	35
TATTGGA	1765	0.0	14.46459	2
TCGGGTA	295	0.0	14.423729	25
TAGATGT	590	0.0	14.423729	4
TAGGTCG	130	4.4492535E-6	14.230769	21
>>END_MODULE