FastQCFastQC Report
Wed 25 May 2016
SRR2088485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107702
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT342621.1024866605614052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT297750.9581034474991489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT239450.770505022682355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125890.40509032075791046No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG90440.29101889434701267No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA82420.2652120441406544No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG77990.25095713810397524No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA74220.23882598781993897No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA68740.22119237944950962No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG61850.19902165651661582No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA45300.14576687211322062No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA38020.12234120259921961No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37550.12082883107839813No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA37300.12002437814179094No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC36860.1186085409733623No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32470.10448234740654026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92500.025.041
GTATTGG12500.018.3520011
ACCGTTA4250.016.5411788
ATTGGAC14250.016.3578953
GTATCAA146000.015.826372
TTGGACC18200.015.5521984
GTCGGGT3000.015.41666724
TATACTG3600.015.4166665
TGGACCC18350.015.22343355
CTAATAC8150.014.9815953
GGACCCT18850.014.8196296
TCGGGTA3150.014.6825425
TATACCG1651.6481863E-814.5757575
GTATTAG7550.014.4569541
GGACCGT5550.014.06
CCGTTAT4800.013.8750019
TAGCGAA4150.013.81927710
CGGGTAA3400.013.60294126
CCGAGTT4100.013.53658513
CGTTTAC4650.013.52688128
Produced by FastQC (version 0.11.5)