##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088485_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3107702
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38146579047798	31.0	31.0	34.0	30.0	34.0
2	31.605832541215342	31.0	31.0	34.0	30.0	34.0
3	31.715153512144987	31.0	31.0	34.0	30.0	34.0
4	35.413291557556036	37.0	35.0	37.0	33.0	37.0
5	35.289005187756096	37.0	35.0	37.0	33.0	37.0
6	35.34872133814632	37.0	35.0	37.0	33.0	37.0
7	35.18929324626364	37.0	35.0	37.0	32.0	37.0
8	35.23633089659176	37.0	35.0	37.0	32.0	37.0
9	36.774584564414475	39.0	37.0	39.0	32.0	39.0
10	36.62470243285875	39.0	35.0	39.0	32.0	39.0
11	36.66992105420661	39.0	35.0	39.0	32.0	39.0
12	36.52170414022966	38.0	35.0	39.0	32.0	39.0
13	36.53530325623242	38.0	35.0	39.0	32.0	39.0
14	37.499368343554174	39.0	36.0	41.0	32.0	41.0
15	37.52744729063469	39.0	36.0	41.0	32.0	41.0
16	37.397983139953574	39.0	36.0	41.0	32.0	41.0
17	37.465629265611696	39.0	36.0	41.0	32.0	41.0
18	37.34135930665167	39.0	36.0	41.0	32.0	41.0
19	37.42757349321138	39.0	36.0	41.0	32.0	41.0
20	37.34360694815655	39.0	36.0	41.0	31.0	41.0
21	37.19000148662903	39.0	36.0	40.0	31.0	41.0
22	37.0990210773105	39.0	36.0	40.0	31.0	41.0
23	36.91296816747552	39.0	36.0	40.0	31.0	41.0
24	37.10143829749442	39.0	36.0	40.0	31.0	41.0
25	37.0608179291322	39.0	36.0	41.0	31.0	41.0
26	36.90419416018653	39.0	36.0	40.0	31.0	41.0
27	36.84253573862616	39.0	35.0	40.0	30.0	41.0
28	36.66452703637607	39.0	35.0	40.0	30.0	41.0
29	36.50118254581681	39.0	35.0	40.0	30.0	41.0
30	36.32041778780591	38.0	35.0	40.0	30.0	41.0
31	36.06689122702241	38.0	35.0	40.0	29.0	41.0
32	35.92691287645984	38.0	35.0	40.0	29.0	41.0
33	35.726797485730614	38.0	34.0	40.0	28.0	41.0
34	35.625061540649654	38.0	34.0	40.0	27.0	41.0
35	35.39586002776328	38.0	34.0	40.0	27.0	41.0
36	35.25713211884537	38.0	34.0	40.0	26.0	41.0
37	35.09795565984126	38.0	34.0	40.0	25.0	41.0
38	35.033811478706774	38.0	34.0	40.0	25.0	41.0
39	34.9492731285046	38.0	33.0	40.0	24.0	41.0
40	34.83429942768	38.0	33.0	40.0	24.0	41.0
41	34.62036964934218	38.0	33.0	40.0	23.0	41.0
42	34.411229905570096	38.0	33.0	40.0	23.0	41.0
43	34.09091058280363	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	7.0
14	7.0
15	24.0
16	43.0
17	99.0
18	270.0
19	606.0
20	1256.0
21	2512.0
22	4832.0
23	8355.0
24	13184.0
25	20372.0
26	29474.0
27	41461.0
28	55792.0
29	72609.0
30	92371.0
31	114657.0
32	140028.0
33	172317.0
34	210355.0
35	257765.0
36	325976.0
37	432564.0
38	557966.0
39	552797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.217948181646754	21.590133159485692	13.65967521982481	23.53224343904274
2	19.071294480616224	23.52181772898431	34.85491852178877	22.551969268610698
3	19.499746114653206	24.865833339232655	32.65300212182506	22.981418424289075
4	13.722293836410312	16.420203738968535	36.68987567018974	33.16762675443141
5	12.336671920280645	38.76053752901662	35.33816948986743	13.564621060835305
6	32.198132253349904	37.78309503292143	13.621898109921737	16.39687460380693
7	26.21081429300493	32.80414273955482	21.661536402138946	19.323506565301308
8	27.015556832669287	34.41308079088664	19.008096657916365	19.56326571852771
9	24.591032216087644	14.267873818017302	21.644964671644836	39.496129294250224
10	16.266714118663888	27.520238426979166	33.883396799307015	22.32965065504994
11	33.322757458726734	22.461967074063086	23.59415413704403	20.621121330166147
12	21.050795732666774	27.235494265537685	30.871364114062416	20.84234588773312
13	31.26715495887315	20.96565243385627	26.17397034850832	21.593222258762264
14	20.52310678436993	22.868537588224353	27.60351539497674	29.00484023242898
15	22.679716394943917	29.670637660882544	25.37051493354253	22.279131010631005
16	22.087510321131177	28.413149008495665	26.363242035433252	23.1360986349399
17	21.823488867336703	26.91860416474939	26.893183451952602	24.364723515961312
18	22.889292473988817	25.87838216148138	29.320604099106028	21.911721265423775
19	22.82654514493346	24.928291065230837	30.260044238475892	21.985119551359816
20	23.68843602121439	24.68331905697522	30.11984418068399	21.5084007411264
21	22.697253468961954	25.532467398740287	30.504694465556863	21.265584666740892
22	21.431623752856613	25.987562514037705	30.102693244075525	22.478120489030157
23	22.410996935999655	26.0926240675586	31.028682930345315	20.467696066096426
24	22.37717773454469	26.124673472553035	28.47792355895128	23.020225233951003
25	22.25313109171986	26.258309194382218	29.80819911304237	21.680360600855554
26	22.098547415421425	26.918250205457277	29.66342976257054	21.31977261655075
27	21.08133276614038	27.014720201615212	29.22838161445338	22.675565417791024
28	21.516220023670225	26.50363516192994	29.596016606482863	22.384128207916977
29	22.048671333351784	26.497650032081584	28.87664261245126	22.577036022115376
30	21.807979014718914	26.617223916578876	30.498226663946543	21.076570404755667
31	22.442853272289298	26.5537686689393	30.18529447160635	20.818083587165052
32	21.929998436143492	26.239774598722786	29.27983442427878	22.550392540854947
33	21.638722116856766	26.314910503001897	30.044611742052485	22.001755638088852
34	21.059129865090025	26.823678718229736	30.43029865797943	21.6868927587008
35	20.595121411255004	28.796808703022364	29.361566842638066	21.24650304308457
36	22.84022084485578	26.589261132502408	29.134743292632304	21.435774730009506
37	21.755399970782268	27.17667266681297	29.13374577099091	21.934181591413846
38	21.547271907023262	25.86467428344159	30.48545195131322	22.102601858221927
39	21.127830145876278	25.772580511258802	31.082999592625033	22.016589750239888
40	20.135328290807806	26.39255630044322	30.729941287806874	22.7421741209421
41	21.42805198181808	25.248527690235424	30.827634052428447	22.49578627551805
42	20.204897380765594	27.060091347239855	30.368001822568573	22.367009449425975
43	20.60911889235197	26.690718736867307	29.666004011967683	23.034158358813038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	154.0
1	320.5
2	487.0
3	1189.5
4	1892.0
5	1892.0
6	3043.0
7	4194.0
8	4726.5
9	5259.0
10	8171.0
11	11083.0
12	11083.0
13	21292.0
14	31501.0
15	50245.5
16	68990.0
17	63334.5
18	57679.0
19	57679.0
20	61791.5
21	65904.0
22	53357.5
23	40811.0
24	46105.0
25	51399.0
26	51399.0
27	57253.0
28	63107.0
29	69740.5
30	76374.0
31	86094.0
32	95814.0
33	95814.0
34	108559.5
35	121305.0
36	133878.5
37	146452.0
38	156055.0
39	165658.0
40	165658.0
41	171506.0
42	177354.0
43	187540.0
44	197726.0
45	204915.5
46	212105.0
47	212105.0
48	264690.0
49	317275.0
50	299780.0
51	282285.0
52	259626.5
53	236968.0
54	236968.0
55	211700.5
56	186433.0
57	159179.0
58	131925.0
59	117397.5
60	102870.0
61	102870.0
62	90577.5
63	78285.0
64	68345.0
65	58405.0
66	49324.0
67	40243.0
68	40243.0
69	34423.0
70	28603.0
71	23848.5
72	19094.0
73	15821.0
74	12548.0
75	12548.0
76	10119.5
77	7691.0
78	6163.0
79	4635.0
80	3569.5
81	2504.0
82	2504.0
83	1909.5
84	1315.0
85	981.5
86	648.0
87	509.5
88	371.0
89	371.0
90	290.5
91	210.0
92	146.5
93	83.0
94	64.0
95	45.0
96	45.0
97	28.5
98	12.0
99	9.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3107702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.75829799800507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96243272947797	39.78794556398799
2	11.579866918481153	11.523889378140519
3	3.627167423742165	5.414450326776569
4	1.5486708005425365	3.0823689277681843
5	0.8055269906946071	2.004082602420926
6	0.47659428021927963	1.4228712131577388
7	0.32148441580022574	1.1197562154171539
8	0.21177974232450084	0.8430239622818593
9	0.1656772128676449	0.7419434516412475
>10	1.0899539034816623	10.457689198271487
>50	0.11871350414942622	4.073766207681387
>100	0.0788193219494027	7.80783651347599
>500	0.008052594036164375	2.7964468167249814
>1k	0.004610759488402862	4.201779123621517
>5k	3.896416469072841E-4	1.4723821456625474
>10k+	2.597610979381894E-4	3.2497683529699355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34262	1.1024866605614052	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29775	0.9581034474991489	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23945	0.770505022682355	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12589	0.40509032075791046	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9044	0.29101889434701267	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8242	0.2652120441406544	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7799	0.25095713810397524	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7422	0.23882598781993897	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6874	0.22119237944950962	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6185	0.19902165651661582	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4530	0.14576687211322062	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3802	0.12234120259921961	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3755	0.12082883107839813	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3730	0.12002437814179094	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3686	0.1186085409733623	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3247	0.10448234740654026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.217811746428712E-5	0.0
3	0.0	0.0	0.0	3.217811746428712E-5	0.0
4	0.0	0.0	0.0	3.217811746428712E-5	0.0
5	0.0	0.0	0.0	3.217811746428712E-5	0.0
6	0.0	0.0	0.0	3.217811746428712E-5	3.217811746428712E-5
7	0.0	0.0	0.0	3.217811746428712E-5	3.217811746428712E-5
8	6.435623492857424E-5	0.0	0.0	3.217811746428712E-5	3.217811746428712E-5
9	6.435623492857424E-5	0.0	0.0	4.5049364450001965E-4	3.217811746428712E-5
10	9.653435239286135E-5	0.0	0.0	5.148498794285939E-4	3.217811746428712E-5
11	9.653435239286135E-5	0.0	0.0	7.079185842143166E-4	3.217811746428712E-5
12	9.653435239286135E-5	0.0	0.0	8.688091715357522E-4	3.217811746428712E-5
13	9.653435239286135E-5	0.0	0.0	9.975216413929005E-4	3.217811746428712E-5
14	9.653435239286135E-5	0.0	0.0	0.0012227684636429105	3.217811746428712E-5
15	9.653435239286135E-5	0.0	0.0	0.0015123715208214944	3.217811746428712E-5
16	9.653435239286135E-5	0.0	0.0	0.001930687047857227	6.435623492857424E-5
17	9.653435239286135E-5	0.0	0.0	0.0024133588098215336	6.435623492857424E-5
18	9.653435239286135E-5	0.0	0.0	0.0026064275146072562	6.435623492857424E-5
19	1.2871246985714848E-4	0.0	0.0	0.003024743041642989	6.435623492857424E-5
20	1.6089058732143559E-4	0.0	0.0	0.005438101851464523	6.435623492857424E-5
21	1.6089058732143559E-4	0.0	0.0	0.008977694772536106	1.2871246985714848E-4
22	1.930687047857227E-4	0.0	0.0	0.014673221563714926	1.2871246985714848E-4
23	1.930687047857227E-4	0.0	0.0	0.018051923897465072	1.2871246985714848E-4
24	1.930687047857227E-4	0.0	0.0	0.023296957044143873	1.2871246985714848E-4
25	1.930687047857227E-4	0.0	0.0	0.027093974904929752	1.2871246985714848E-4
26	2.2524682225000982E-4	0.0	0.0	0.03278950169610857	1.2871246985714848E-4
27	2.2524682225000982E-4	0.0	0.0	0.08186113082914642	1.2871246985714848E-4
28	2.2524682225000982E-4	0.0	0.0	0.17903904557129352	1.2871246985714848E-4
29	2.2524682225000982E-4	0.0	0.0	0.2974545178398701	1.2871246985714848E-4
30	2.2524682225000982E-4	0.0	0.0	0.4633005352508059	1.2871246985714848E-4
31	2.2524682225000982E-4	3.217811746428712E-5	0.0	0.6520251941788499	1.2871246985714848E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9250	0.0	25.04	1
GTATTGG	1250	0.0	18.352001	1
ACCGTTA	425	0.0	16.541178	8
ATTGGAC	1425	0.0	16.357895	3
GTATCAA	14600	0.0	15.82637	2
TTGGACC	1820	0.0	15.552198	4
GTCGGGT	300	0.0	15.416667	24
TATACTG	360	0.0	15.416666	5
TGGACCC	1835	0.0	15.2234335	5
CTAATAC	815	0.0	14.981595	3
GGACCCT	1885	0.0	14.819629	6
TCGGGTA	315	0.0	14.68254	25
TATACCG	165	1.6481863E-8	14.575757	5
GTATTAG	755	0.0	14.456954	1
GGACCGT	555	0.0	14.0	6
CCGTTAT	480	0.0	13.875001	9
TAGCGAA	415	0.0	13.819277	10
CGGGTAA	340	0.0	13.602941	26
CCGAGTT	410	0.0	13.536585	13
CGTTTAC	465	0.0	13.526881	28
>>END_MODULE