FastQCFastQC Report
Wed 25 May 2016
SRR2088484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390050
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT418011.2330496600345127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT363751.0729930237017153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT294580.8689547351808969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147050.43376941343048037No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG109760.3237710358254303No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102090.30114600079644843No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG97630.28798985265704047No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA87970.25949469771832273No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA85090.250999247798705No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG80580.23769560920930372No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA58230.1717673780622705No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA49600.14631052639341602No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC48070.14179731862361913No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47280.139466969513724No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44000.12979159599415938No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38160.1125647114349346No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37890.11176826300497043No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG37170.109644400525066No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG35570.10492470612527838No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35060.10342030353534609No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG34890.10291883600536866No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.10032300408548546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110600.024.2540681
TATACCG1550.021.483875
GTATTGG13050.018.9961681
TGTTACG3700.018.016
ACCGTTA5500.017.8272728
CGTTTAC5450.017.65137728
CCGTTTA5450.017.31192627
CGTTATT4000.017.112510
TAATACT10250.016.4243894
GTATTAG9050.015.7403321
CTAATAC9650.015.5284983
ATTGGAC16800.015.4166683
GTATCAA175050.015.3136232
CTCTAAT8700.015.3103461
ATACTGG9800.015.2908156
GGACCGT7750.015.277426
TTGGACC22350.014.9821034
GACCGTT6550.014.9694667
TACCCCG4950.014.9494935
TGGACCC20850.014.9064755
Produced by FastQC (version 0.11.5)