##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088484_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3390050
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37757820681111	31.0	31.0	34.0	30.0	34.0
2	31.59483635934573	31.0	31.0	34.0	30.0	34.0
3	31.70183153640802	31.0	31.0	34.0	30.0	34.0
4	35.39985516437811	37.0	35.0	37.0	33.0	37.0
5	35.276181767230575	37.0	35.0	37.0	33.0	37.0
6	35.34118995295054	37.0	35.0	37.0	33.0	37.0
7	35.17525729709001	37.0	35.0	37.0	32.0	37.0
8	35.2243550980074	37.0	35.0	37.0	32.0	37.0
9	36.759687320246016	39.0	37.0	39.0	32.0	39.0
10	36.614993289184525	39.0	35.0	39.0	32.0	39.0
11	36.66277105057448	39.0	35.0	39.0	32.0	39.0
12	36.506042978717126	38.0	35.0	39.0	32.0	39.0
13	36.522768395746375	38.0	35.0	39.0	32.0	39.0
14	37.463899352516925	39.0	36.0	41.0	32.0	41.0
15	37.48923201722688	39.0	36.0	41.0	32.0	41.0
16	37.35525641214731	39.0	36.0	41.0	32.0	41.0
17	37.44388578339552	39.0	36.0	41.0	32.0	41.0
18	37.317891476526896	39.0	36.0	41.0	32.0	41.0
19	37.414386513473254	39.0	36.0	41.0	32.0	41.0
20	37.327437058450464	39.0	36.0	41.0	31.0	41.0
21	37.17370569755608	39.0	36.0	40.0	31.0	41.0
22	37.08856536039291	39.0	36.0	40.0	31.0	41.0
23	36.892574740785534	39.0	36.0	40.0	31.0	41.0
24	37.077623338888806	39.0	36.0	40.0	31.0	41.0
25	37.03477765814664	39.0	36.0	41.0	31.0	41.0
26	36.866206693116624	39.0	36.0	40.0	30.0	41.0
27	36.80558398843675	39.0	35.0	40.0	30.0	41.0
28	36.62111119305025	39.0	35.0	40.0	30.0	41.0
29	36.45160543354818	39.0	35.0	40.0	30.0	41.0
30	36.25408799280247	38.0	35.0	40.0	30.0	41.0
31	35.98173891240542	38.0	35.0	40.0	29.0	41.0
32	35.83896402707925	38.0	35.0	40.0	29.0	41.0
33	35.62490199259598	38.0	34.0	40.0	27.0	41.0
34	35.515848438813585	38.0	34.0	40.0	27.0	41.0
35	35.27353755844309	38.0	34.0	40.0	26.0	41.0
36	35.129469181870476	38.0	34.0	40.0	25.0	41.0
37	34.95525493724281	38.0	33.0	40.0	25.0	41.0
38	34.8822099969027	38.0	33.0	40.0	24.0	41.0
39	34.785253019866964	38.0	33.0	40.0	24.0	41.0
40	34.652513384758336	38.0	33.0	40.0	24.0	41.0
41	34.429662394359966	38.0	33.0	40.0	23.0	41.0
42	34.187032639636584	38.0	33.0	40.0	22.0	41.0
43	33.86395067919352	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	3.0
13	7.0
14	14.0
15	30.0
16	65.0
17	129.0
18	313.0
19	684.0
20	1478.0
21	2958.0
22	5623.0
23	9486.0
24	15099.0
25	22899.0
26	33054.0
27	46932.0
28	62165.0
29	82138.0
30	102969.0
31	126973.0
32	155504.0
33	190887.0
34	232087.0
35	283256.0
36	357746.0
37	472353.0
38	598324.0
39	586871.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54360555154054	21.261574313063228	13.816610374478252	23.378209760917983
2	19.215380304125308	23.501393784752437	34.68916387663899	22.59406203448327
3	19.674311588324656	24.55397413017507	32.916122181088774	22.8555921004115
4	13.746434418371411	16.28509903983717	36.266102269878026	33.702364271913396
5	12.368962109703396	38.6977478208286	35.13172372088908	13.80156634857893
6	32.48185130012832	37.311190100440996	13.302901137151368	16.90405746227932
7	26.195277355791212	33.02396719812392	21.21287296647542	19.567882479609445
8	27.428533502455714	34.01259568442943	18.747658589106354	19.811212224008496
9	24.591554696833377	14.168699576702409	21.607704901107653	39.632040825356555
10	16.545655668795444	27.47685137387354	33.46873939912391	22.508753558207108
11	33.42968392796566	22.195896815681184	23.510390702202034	20.86402855415112
12	21.460863409094262	27.169215793277385	30.57087653574431	20.799044261884042
13	31.807495464668662	20.80373445819383	26.02758071414876	21.361189362988746
14	20.635624843291396	22.850282444211736	27.61502042742732	28.899072285069543
15	22.807244730903676	29.482986976593267	25.51817819796168	22.19159009454138
16	22.12929012846418	28.202386395480893	26.38701494078258	23.28130853527234
17	22.011179776109497	26.640728012861164	26.71824899337768	24.629843217651658
18	23.277473783572514	25.367767437058454	29.411454108346486	21.943304671022553
19	22.954971165617028	24.545980147785432	30.518959897346647	21.980088789250896
20	23.965280748071564	24.204657748410792	30.37465524107314	21.455406262444505
21	22.80063715874397	25.080485538561376	30.881904396690313	21.236972906004336
22	21.47198418902376	25.59381130071828	30.32070323446557	22.61350127579239
23	22.50152652615743	25.724458341322396	31.34384448606953	20.430170646450645
24	22.379876403002903	25.810386277488533	28.670078612409846	23.139658707098715
25	22.29064468075692	25.87513458503562	30.008170970929633	21.826049763277826
26	22.092712496865825	26.744089320216517	29.88557690889515	21.277621274022508
27	20.999631273875018	26.758277901505878	29.42402029468592	22.818070529933188
28	21.51953511010162	26.27760652497751	29.685904337694137	22.51695402722674
29	22.014778543089335	26.22114718072005	28.91511924602882	22.848955030161797
30	21.82463385495789	26.451114290349697	30.769870650875358	20.95438120381705
31	22.64795504491084	26.219583781950117	30.454506570699547	20.677954602439492
32	22.058081739207385	25.916048435863775	29.416527779826257	22.60934204510258
33	21.681155145204347	26.114629577734842	30.091709561805875	22.112505715254937
34	21.016356690904264	26.655683544490493	30.55598000029498	21.771979764310263
35	20.49636436040766	28.872759988790726	29.38685270128759	21.24402294951402
36	22.989926402265453	26.41309125234141	29.140750136428668	21.456232208964472
37	21.73861742452176	27.207032344655683	29.1533458208581	21.901004409964457
38	21.585492839338652	25.68357398858424	30.645418209170955	22.085514962906153
39	21.167386911697466	25.50573000398224	31.23402309700447	22.092859987315823
40	20.01530950870931	26.232179466379552	30.945030309287475	22.80748071562366
41	21.561274907449743	24.993053199805313	30.953879736287078	22.49179215645787
42	20.135514225453903	27.040427132343183	30.520965767466556	22.30309287473636
43	20.609932006902554	26.723558649577438	29.635079128626423	23.031430214893586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	361.0
2	561.0
3	1317.5
4	2074.0
5	2074.0
6	3458.0
7	4842.0
8	5542.5
9	6243.0
10	9599.5
11	12956.0
12	12956.0
13	24869.0
14	36782.0
15	59963.5
16	83145.0
17	75856.5
18	68568.0
19	68568.0
20	73138.5
21	77709.0
22	61474.0
23	45239.0
24	50279.5
25	55320.0
26	55320.0
27	61001.5
28	66683.0
29	72715.0
30	78747.0
31	88254.5
32	97762.0
33	97762.0
34	109749.5
35	121737.0
36	134669.5
37	147602.0
38	157862.5
39	168123.0
40	168123.0
41	173433.0
42	178743.0
43	190598.0
44	202453.0
45	213527.0
46	224601.0
47	224601.0
48	291621.0
49	358641.0
50	338756.0
51	318871.0
52	293373.5
53	267876.0
54	267876.0
55	238823.5
56	209771.0
57	178081.0
58	146391.0
59	129763.0
60	113135.0
61	113135.0
62	100889.5
63	88644.0
64	77335.5
65	66027.0
66	56765.5
67	47504.0
68	47504.0
69	40805.5
70	34107.0
71	28661.5
72	23216.0
73	19154.5
74	15093.0
75	15093.0
76	12187.0
77	9281.0
78	7291.0
79	5301.0
80	4111.0
81	2921.0
82	2921.0
83	2226.5
84	1532.0
85	1180.5
86	829.0
87	627.0
88	425.0
89	425.0
90	344.5
91	264.0
92	182.5
93	101.0
94	70.5
95	40.0
96	40.0
97	31.5
98	23.0
99	14.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3390050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.821524169383125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75304135600109	36.87337429262435
2	12.235531322304004	11.457724510650024
3	3.801332018823986	5.339524769856515
4	1.649263528973739	3.0888412873410407
5	0.8935947980175688	2.091973521650732
6	0.5120278954899642	1.438435589044904
7	0.3342423775706124	1.095481629189816
8	0.2507588613184092	0.939272966872551
9	0.18020270887350304	0.759362894001813
>10	1.1492376327589438	10.482625041171989
>50	0.1335902115742054	4.342632646101337
>100	0.09173345882783306	8.551939250792941
>500	0.009304274309805202	3.044266301793191
>1k	0.005443316942891018	5.027585467351004
>5k	3.164719152843615E-4	1.2135694195512097
>10k+	3.797662983412338E-4	4.25339041200654
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41801	1.2330496600345127	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36375	1.0729930237017153	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29458	0.8689547351808969	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14705	0.43376941343048037	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10976	0.3237710358254303	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10209	0.30114600079644843	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9763	0.28798985265704047	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8797	0.25949469771832273	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8509	0.250999247798705	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8058	0.23769560920930372	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5823	0.1717673780622705	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4960	0.14631052639341602	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4807	0.14179731862361913	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4728	0.139466969513724	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4400	0.12979159599415938	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3816	0.1125647114349346	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3789	0.11176826300497043	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3717	0.109644400525066	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3557	0.10492470612527838	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3506	0.10342030353534609	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3489	0.10291883600536866	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3401	0.10032300408548546	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.899617999734517E-5	0.0	0.0	0.0	0.0
7	5.899617999734517E-5	0.0	0.0	0.0	0.0
8	5.899617999734517E-5	0.0	0.0	2.9498089998672585E-5	0.0
9	5.899617999734517E-5	2.9498089998672585E-5	0.0	1.4749044999336292E-4	0.0
10	8.849426999601775E-5	2.9498089998672585E-5	0.0	1.4749044999336292E-4	0.0
11	8.849426999601775E-5	2.9498089998672585E-5	0.0	3.53977079984071E-4	0.0
12	8.849426999601775E-5	2.9498089998672585E-5	0.0	5.309656199761066E-4	0.0
13	8.849426999601775E-5	2.9498089998672585E-5	0.0	5.604637099747791E-4	0.0
14	8.849426999601775E-5	2.9498089998672585E-5	0.0	7.964484299641598E-4	0.0
15	8.849426999601775E-5	2.9498089998672585E-5	0.0	0.0010324331499535406	0.0
16	8.849426999601775E-5	2.9498089998672585E-5	0.0	0.0011799235999469034	0.0
17	8.849426999601775E-5	2.9498089998672585E-5	0.0	0.0016518930399256648	0.0
18	1.1799235999469034E-4	2.9498089998672585E-5	0.0	0.0020943643899057536	0.0
19	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.002979307089865931	0.0
20	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.004837686759782304	0.0
21	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.008141472839633634	2.9498089998672585E-5
22	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.013038155779413283	2.9498089998672585E-5
23	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.01572248196929249	2.9498089998672585E-5
24	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.020943643899057535	2.9498089998672585E-5
25	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.025014380318874353	2.9498089998672585E-5
26	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.032536393268535864	2.9498089998672585E-5
27	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.08389256795622484	2.9498089998672585E-5
28	1.4749044999336292E-4	2.9498089998672585E-5	0.0	0.18288815799177002	2.9498089998672585E-5
29	1.4749044999336292E-4	5.899617999734517E-5	0.0	0.30651465317620685	2.9498089998672585E-5
30	1.4749044999336292E-4	5.899617999734517E-5	0.0	0.48875385318800607	2.9498089998672585E-5
31	1.4749044999336292E-4	5.899617999734517E-5	0.0	0.677866108169496	2.9498089998672585E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11060	0.0	24.254068	1
TATACCG	155	0.0	21.48387	5
GTATTGG	1305	0.0	18.996168	1
TGTTACG	370	0.0	18.0	16
ACCGTTA	550	0.0	17.827272	8
CGTTTAC	545	0.0	17.651377	28
CCGTTTA	545	0.0	17.311926	27
CGTTATT	400	0.0	17.1125	10
TAATACT	1025	0.0	16.424389	4
GTATTAG	905	0.0	15.740332	1
CTAATAC	965	0.0	15.528498	3
ATTGGAC	1680	0.0	15.416668	3
GTATCAA	17505	0.0	15.313623	2
CTCTAAT	870	0.0	15.310346	1
ATACTGG	980	0.0	15.290815	6
GGACCGT	775	0.0	15.27742	6
TTGGACC	2235	0.0	14.982103	4
GACCGTT	655	0.0	14.969466	7
TACCCCG	495	0.0	14.949493	5
TGGACCC	2085	0.0	14.906475	5
>>END_MODULE