FastQCFastQC Report
Wed 25 May 2016
SRR2088483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637998
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161970.9888290461893114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135000.8241768304967405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107530.6564721080245519No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61920.3780224395878383No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36040.22002468867483357No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32690.19957289325139593No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32240.19682563714974013No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA30920.18876701925154976No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27720.1692309758619974No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG26180.15982925498077533No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18060.11025654487978617No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17120.10451783213410518No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA16620.10146532535448761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45500.023.1758251
TCGGTTC502.7027822E-422.19999913
CGTTGAC1002.8767863E-718.49999832
GTATTAG4150.017.8313261
ATACTGG5000.017.396
GTATTGG5350.016.9439241
CTAATAC5550.016.6666663
TATACTG1708.54925E-1116.323535
ATTAGAG3200.016.18753
ATGCTAG4450.015.38202315
TAATACT5550.015.3333334
TGTTACG2201.8189894E-1215.13636416
AATACTG6000.015.1083345
TTATGCG2350.014.9574474
CGTTATT2251.8189894E-1214.810
ACCGTTA2750.014.88
TAACGTA1001.09381464E-414.79999923
TACTGGT5400.014.7314827
GTATCAA71900.014.6404732
GTATACT1405.998827E-714.5357144
Produced by FastQC (version 0.11.5)