##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1637998
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33602910382064	31.0	31.0	34.0	30.0	34.0
2	31.56411179989231	31.0	31.0	34.0	30.0	34.0
3	31.675477625735805	31.0	31.0	34.0	30.0	34.0
4	35.388585334048024	37.0	35.0	37.0	33.0	37.0
5	35.25343986988995	37.0	35.0	37.0	33.0	37.0
6	35.306113316377676	37.0	35.0	37.0	33.0	37.0
7	35.14952826560228	37.0	35.0	37.0	32.0	37.0
8	35.18699961782615	37.0	35.0	37.0	32.0	37.0
9	36.73200394628076	39.0	35.0	39.0	32.0	39.0
10	36.56724122984277	38.0	35.0	39.0	32.0	39.0
11	36.61008621500148	38.0	35.0	39.0	32.0	39.0
12	36.46234671837206	38.0	35.0	39.0	32.0	39.0
13	36.480046373682995	38.0	35.0	39.0	32.0	39.0
14	37.445096392059085	39.0	36.0	41.0	32.0	41.0
15	37.45684915366197	39.0	36.0	41.0	32.0	41.0
16	37.32788928924211	39.0	36.0	41.0	32.0	41.0
17	37.38567202157756	39.0	36.0	41.0	32.0	41.0
18	37.25381838073062	39.0	36.0	40.0	31.0	41.0
19	37.340632284044304	39.0	36.0	40.0	31.0	41.0
20	37.25037149007508	39.0	36.0	40.0	31.0	41.0
21	37.087939667814005	39.0	36.0	40.0	31.0	41.0
22	37.00166300569354	39.0	36.0	40.0	31.0	41.0
23	36.81181723054607	39.0	36.0	40.0	30.0	41.0
24	37.005600739439245	39.0	36.0	40.0	31.0	41.0
25	36.963944400420516	39.0	36.0	40.0	31.0	41.0
26	36.79668900694629	39.0	36.0	40.0	30.0	41.0
27	36.73835621288915	39.0	35.0	40.0	30.0	41.0
28	36.55815269615714	39.0	35.0	40.0	30.0	41.0
29	36.38615309664603	39.0	35.0	40.0	30.0	41.0
30	36.213373276402045	38.0	35.0	40.0	30.0	41.0
31	35.9690237717018	38.0	35.0	40.0	29.0	41.0
32	35.82260601050795	38.0	34.0	40.0	28.0	41.0
33	35.628096615502585	38.0	34.0	40.0	27.0	41.0
34	35.52546889556642	38.0	34.0	40.0	27.0	41.0
35	35.31751442919955	38.0	34.0	40.0	26.0	41.0
36	35.17936163536219	38.0	34.0	40.0	25.0	41.0
37	35.01533396255673	38.0	33.0	40.0	25.0	41.0
38	34.959081757120586	38.0	33.0	40.0	25.0	41.0
39	34.86643878686055	38.0	33.0	40.0	24.0	41.0
40	34.76809678644296	38.0	33.0	40.0	24.0	41.0
41	34.57219789035152	38.0	33.0	40.0	23.0	41.0
42	34.37279227447164	38.0	33.0	40.0	23.0	41.0
43	34.048486628188805	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	8.0
15	6.0
16	24.0
17	72.0
18	130.0
19	359.0
20	725.0
21	1471.0
22	2622.0
23	4502.0
24	7427.0
25	11073.0
26	16123.0
27	22274.0
28	30166.0
29	39359.0
30	49995.0
31	61849.0
32	76088.0
33	92971.0
34	113328.0
35	138321.0
36	172153.0
37	226380.0
38	291819.0
39	278749.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15566685673609	21.568341353286147	13.479808888655542	23.796182901322226
2	19.355762339148157	23.510101965936467	34.348637788324524	22.78549790659085
3	19.573161871992518	24.846550484188626	32.4228723111994	23.157415332619454
4	13.972727683428184	16.65209603430529	36.6579202172408	32.717256065025715
5	12.507890730025311	38.82300222588794	35.168724259736585	13.500382784350165
6	32.27647408604894	37.924771580917685	13.857892378378972	15.9408619546544
7	26.35583193630273	32.69875787394124	21.79611940918121	19.149290780574823
8	27.05326868530975	34.473180064932926	19.070902406474243	19.402648843283078
9	24.98214283533924	14.247758544271727	21.307046772950883	39.46305184743815
10	16.362107890241624	27.505711240184667	33.81719635799311	22.314984511580597
11	33.71341112748611	22.551553787000962	23.318953991396814	20.41608109411611
12	21.037632524581838	27.18391597547738	30.822504056781508	20.95594744315927
13	30.916521265593733	21.134702240173674	25.90979964566501	22.038976848567582
14	20.741539366958932	22.776828787336736	27.35894671422065	29.122685131483678
15	23.102164959908375	29.60339389913785	25.001312577915236	22.29312856303854
16	22.359306910020646	28.30693321969868	26.149116177187032	23.184643693093644
17	22.05344573070297	27.10216984391922	26.79612551419477	24.048258911183044
18	22.803324546183816	26.21346302010137	28.887275808639572	22.095936625075243
19	23.08562037316285	25.308516860215946	29.702661419611015	21.903201347010192
20	23.682019147764528	25.170360403370456	29.544846819104787	21.602773629760232
21	22.72804972899845	25.9258558313258	29.874883852117033	21.471210587558716
22	21.78549668558814	26.336479043319954	29.48526188676665	22.392762384325255
23	22.577316944220932	26.39221781711577	30.180195580214388	20.850269658448912
24	22.574813888661645	26.57964173338429	27.9627936053646	22.882750772589468
25	22.423104301714655	26.485929775250032	29.202599758974063	21.888366164061253
26	22.17994161165032	27.138006273511934	29.15009664236464	21.531955472473104
27	21.50252930711759	27.13263386157981	28.64930238010059	22.715534451202014
28	21.802834924096366	26.866455270397154	28.988985334536427	22.34172447097005
29	22.305582790699376	26.80345153046585	28.401866180544786	22.489099498289985
30	22.030063528771095	26.902230649854275	29.77634893327098	21.29135688810365
31	22.446486503646522	26.86920252649881	29.628729705408674	21.055581264445987
32	22.00814653009344	26.593500114163753	28.830804433216645	22.567548922526157
33	21.74245633999553	26.71260892870443	29.36651937303953	22.178415358260512
34	21.300270207900134	26.859190304261666	29.948876616454967	21.89166287138324
35	21.006985356514477	28.48147555735721	28.928545700300003	21.58299338582831
36	22.78983246621791	26.607053244265256	28.748753050980525	21.85436123853631
37	21.77988007311364	27.056076991546995	28.919266079689965	22.244776855649395
38	21.663945865623766	25.88843209820769	30.14478650157082	22.302835534597722
39	21.1576570911564	25.941850966851	30.655592986072023	22.24489895592058
40	20.436716039946326	26.403023691115617	30.380562125228476	22.779698143709577
41	21.27481230135812	25.312607219300638	30.617497701462394	22.79508277787885
42	20.27145332289783	26.862792262261614	30.172503263129748	22.693251151710808
43	20.638975139163783	26.385197051522653	29.782148696152255	23.193679113161313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	96.0
1	188.0
2	280.0
3	585.0
4	890.0
5	890.0
6	1450.5
7	2011.0
8	2345.5
9	2680.0
10	4173.0
11	5666.0
12	5666.0
13	10784.5
14	15903.0
15	24762.0
16	33621.0
17	30740.5
18	27860.0
19	27860.0
20	30228.0
21	32596.0
22	27208.0
23	21820.0
24	24728.5
25	27637.0
26	27637.0
27	31055.0
28	34473.0
29	38111.0
30	41749.0
31	46795.0
32	51841.0
33	51841.0
34	58815.0
35	65789.0
36	72497.5
37	79206.0
38	84638.0
39	90070.0
40	90070.0
41	92739.5
42	95409.0
43	101110.5
44	106812.0
45	109091.5
46	111371.0
47	111371.0
48	133441.5
49	155512.0
50	147697.0
51	139882.0
52	129516.0
53	119150.0
54	119150.0
55	107729.5
56	96309.0
57	84125.5
58	71942.0
59	64964.0
60	57986.0
61	57986.0
62	51364.5
63	44743.0
64	39439.0
65	34135.0
66	29017.0
67	23899.0
68	23899.0
69	20529.0
70	17159.0
71	14427.0
72	11695.0
73	9507.5
74	7320.0
75	7320.0
76	6002.5
77	4685.0
78	3656.0
79	2627.0
80	2078.5
81	1530.0
82	1530.0
83	1152.0
84	774.0
85	605.0
86	436.0
87	338.5
88	241.0
89	241.0
90	181.5
91	122.0
92	80.0
93	38.0
94	28.0
95	18.0
96	18.0
97	14.5
98	11.0
99	7.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1637998.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.28171819992741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.45530673664594	46.97008056040411
2	9.953674876161067	11.20419848867589
3	2.7378222919224826	4.622680281463817
4	1.156088754134954	2.602666458973146
5	0.6034261047933351	1.6980928992229176
6	0.3764791760174972	1.271333693565459
7	0.25927174170677525	1.0214581372761564
8	0.19611758951318514	0.8830267925624101
9	0.14326135592806352	0.7256695736954495
>10	0.9488561389294962	10.269198541213335
>50	0.09403694038660579	3.6931759246203906
>100	0.0665141440513904	7.3746903613771595
>500	0.006313817725416542	2.374122372761986
>1k	0.0023948963786062745	2.431959057146175
>5k	1.0885892630028521E-4	0.3793694382488314
>10k+	3.2657677890085557E-4	2.4782774187928345
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16197	0.9888290461893114	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13500	0.8241768304967405	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10753	0.6564721080245519	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6192	0.3780224395878383	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3604	0.22002468867483357	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3269	0.19957289325139593	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3224	0.19682563714974013	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3092	0.18876701925154976	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2772	0.1692309758619974	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2618	0.15982925498077533	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1806	0.11025654487978617	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1712	0.10451783213410518	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1662	0.10146532535448761	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.105013559235116E-5	0.0
4	0.0	0.0	0.0	6.105013559235116E-5	0.0
5	0.0	0.0	0.0	6.105013559235116E-5	0.0
6	6.105013559235116E-5	0.0	0.0	6.105013559235116E-5	0.0
7	6.105013559235116E-5	0.0	0.0	1.221002711847023E-4	0.0
8	1.221002711847023E-4	0.0	0.0	1.221002711847023E-4	0.0
9	1.221002711847023E-4	0.0	0.0	4.2735094914645805E-4	0.0
10	1.221002711847023E-4	0.0	0.0	6.105013559235115E-4	0.0
11	1.221002711847023E-4	0.0	0.0	6.105013559235115E-4	0.0
12	1.221002711847023E-4	0.0	0.0	6.715514915158627E-4	0.0
13	1.221002711847023E-4	0.0	0.0	8.547018982929161E-4	0.0
14	1.8315040677705345E-4	0.0	0.0	9.157520338852672E-4	0.0
15	1.8315040677705345E-4	0.0	0.0	0.0010989024406623208	0.0
16	2.442005423694046E-4	0.0	0.0	0.001221002711847023	0.0
17	2.442005423694046E-4	0.0	0.0	0.0018315040677705345	0.0
18	2.442005423694046E-4	0.0	0.0	0.0020757046101399393	0.0
19	2.442005423694046E-4	0.0	0.0	0.0025641056948787484	0.0
20	2.442005423694046E-4	0.0	0.0	0.004212459355872229	0.0
21	2.442005423694046E-4	0.0	0.0	0.007020765593120383	0.0
22	2.442005423694046E-4	0.0	0.0	0.012271077254062582	0.0
23	2.442005423694046E-4	0.0	0.0	0.014468882135387223	0.0
24	2.442005423694046E-4	0.0	0.0	0.018925542033628857	0.0
25	2.442005423694046E-4	0.0	0.0	0.022588550169169927	0.0
26	2.442005423694046E-4	0.0	0.0	0.030402967524990873	0.0
27	2.442005423694046E-4	0.0	0.0	0.0819903321005276	0.0
28	2.442005423694046E-4	0.0	0.0	0.17527493928564014	0.0
29	2.442005423694046E-4	0.0	0.0	0.2948111047754637	0.0
30	2.442005423694046E-4	0.0	0.0	0.4660567351120087	0.0
31	2.442005423694046E-4	0.0	0.0	0.6573268099228449	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4550	0.0	23.175825	1
TCGGTTC	50	2.7027822E-4	22.199999	13
CGTTGAC	100	2.8767863E-7	18.499998	32
GTATTAG	415	0.0	17.831326	1
ATACTGG	500	0.0	17.39	6
GTATTGG	535	0.0	16.943924	1
CTAATAC	555	0.0	16.666666	3
TATACTG	170	8.54925E-11	16.32353	5
ATTAGAG	320	0.0	16.1875	3
ATGCTAG	445	0.0	15.382023	15
TAATACT	555	0.0	15.333333	4
TGTTACG	220	1.8189894E-12	15.136364	16
AATACTG	600	0.0	15.108334	5
TTATGCG	235	0.0	14.957447	4
CGTTATT	225	1.8189894E-12	14.8	10
ACCGTTA	275	0.0	14.8	8
TAACGTA	100	1.09381464E-4	14.799999	23
TACTGGT	540	0.0	14.731482	7
GTATCAA	7190	0.0	14.640473	2
GTATACT	140	5.998827E-7	14.535714	4
>>END_MODULE