FastQCFastQC Report
Wed 25 May 2016
SRR2088482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452308
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54841.2124481547971737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47261.0448632347869151No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37360.825985832662699No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.4565473084712187No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG11620.25690458713973663No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG11540.255135880859945No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA10610.23457467035736712No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9960.22020393183405998No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA9870.21821413726929437No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG9310.20583319331075284No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.1406121492434359No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA6010.13287405926934742No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA5770.1275679404299725No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC5460.12071420359577986No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.11474481990148305No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.11430264333153516No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA5130.11341829019163932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAG250.00549286329.63
TAGTACT856.91216E-1126.1176454
TATACCG451.3214794E-424.6666665
GGTATCA16800.024.226191
AGGTTAC400.001929386523.12534
ATACCGC400.001929386523.1256
CGGTTGG603.7198566E-521.58333433
AAGACTA450.003822274720.5555555
ATTGTAC450.003822274720.5555553
CGGGAAC555.1364687E-420.1818186
TCGGGTA656.890307E-519.92307725
TTGGACC2700.019.870374
TGTGACG759.24541E-619.73333410
CGGGTAA701.2171241E-418.526
AGTCAAT609.225011E-418.527
TCGTCTT500.00702844718.514
GTACTAT609.225011E-418.51
TATACTT902.1463493E-618.55
ATTTCGG701.2171241E-418.529
TGGACCC3050.018.196725
Produced by FastQC (version 0.11.5)