##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452308
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432470794237556	31.0	31.0	34.0	30.0	34.0
2	31.63242525005085	31.0	31.0	34.0	30.0	34.0
3	31.740707659382544	31.0	31.0	34.0	30.0	34.0
4	35.42459120776108	37.0	35.0	37.0	33.0	37.0
5	35.320531584672395	37.0	35.0	37.0	33.0	37.0
6	35.38130212156318	37.0	35.0	37.0	33.0	37.0
7	35.20721941685754	37.0	35.0	37.0	32.0	37.0
8	35.257110199244764	37.0	35.0	37.0	32.0	37.0
9	36.803361868461316	39.0	37.0	39.0	32.0	39.0
10	36.65443458882001	39.0	35.0	39.0	32.0	39.0
11	36.681971134713514	39.0	35.0	39.0	32.0	39.0
12	36.528997939457184	38.0	35.0	39.0	32.0	39.0
13	36.54605047887723	38.0	35.0	39.0	32.0	39.0
14	37.50033826507601	39.0	36.0	41.0	32.0	41.0
15	37.537120723047124	39.0	36.0	41.0	32.0	41.0
16	37.392414018765976	39.0	36.0	41.0	32.0	41.0
17	37.46360444652759	39.0	36.0	41.0	32.0	41.0
18	37.33841983780963	39.0	36.0	41.0	32.0	41.0
19	37.426804301493675	39.0	36.0	41.0	32.0	41.0
20	37.32790487897627	39.0	36.0	41.0	31.0	41.0
21	37.16457369756891	39.0	36.0	40.0	31.0	41.0
22	37.065725125357055	39.0	36.0	40.0	31.0	41.0
23	36.86733155283568	39.0	36.0	40.0	30.0	41.0
24	37.06492920753115	39.0	36.0	40.0	31.0	41.0
25	37.030658312477335	39.0	36.0	41.0	31.0	41.0
26	36.84058208123668	39.0	36.0	40.0	30.0	41.0
27	36.791288237218886	39.0	35.0	40.0	30.0	41.0
28	36.592439222830464	39.0	35.0	40.0	30.0	41.0
29	36.417171484917354	39.0	35.0	40.0	30.0	41.0
30	36.24199439320109	38.0	35.0	40.0	30.0	41.0
31	35.93922061957781	38.0	35.0	40.0	29.0	41.0
32	35.786824464745266	38.0	34.0	40.0	28.0	41.0
33	35.55117088355722	38.0	34.0	40.0	27.0	41.0
34	35.43578048586362	38.0	34.0	40.0	27.0	41.0
35	35.185621302298436	38.0	34.0	40.0	26.0	41.0
36	35.02308824959983	38.0	34.0	40.0	25.0	41.0
37	34.85879533415283	38.0	33.0	40.0	25.0	41.0
38	34.76304863057916	38.0	33.0	40.0	24.0	41.0
39	34.6583279535184	38.0	33.0	40.0	24.0	41.0
40	34.508438939837454	38.0	33.0	40.0	23.0	41.0
41	34.28015644207045	38.0	33.0	40.0	22.0	41.0
42	34.031774808316456	38.0	33.0	40.0	21.0	41.0
43	33.68869221857672	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	5.0
16	3.0
17	19.0
18	32.0
19	89.0
20	211.0
21	401.0
22	769.0
23	1298.0
24	2108.0
25	3192.0
26	4681.0
27	6355.0
28	8538.0
29	11025.0
30	13852.0
31	16932.0
32	21077.0
33	25306.0
34	30862.0
35	37220.0
36	47205.0
37	63280.0
38	79420.0
39	78424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41337318818151	21.41483237086233	13.761640298203877	23.410154142752283
2	19.38590517965634	23.685630145829833	34.51961937440859	22.408845300105238
3	19.71775869540225	24.783112392440547	32.604552649964184	22.89457626219302
4	13.907779654571664	16.53320303863739	36.19148898538164	33.36752832140931
5	12.39907319790939	38.926351070509476	34.97174491718033	13.702830814400807
6	32.250590305720884	37.48772960018395	13.50517788763409	16.756502206461086
7	25.97256736560043	32.986372118114204	21.524934336779364	19.516126179506
8	27.35547458811253	34.32506168363151	18.788082457086762	19.531381271169206
9	24.789523952704794	14.37493920072163	21.485801710338972	39.34973513623461
10	16.842063372746004	27.510236387594293	33.06375301785509	22.583947221804614
11	33.31623583929535	22.596549254048128	23.188402592923406	20.89881231373312
12	21.027485695587963	27.538535688070958	30.64438391538509	20.789594700955984
13	31.504417343933778	21.149968605463535	25.957091185652253	21.38852286495043
14	20.58641456706492	23.160103292446742	27.386869124578826	28.866613015909515
15	22.95847077655049	29.663857371525598	25.34998275511377	22.02768909681014
16	22.01376053485678	28.34506575165595	26.412533052698606	23.228640660788667
17	21.911175570628863	26.93540684666201	26.737311743325343	24.416105839383782
18	22.88131096509458	25.84101983604093	29.21615359445334	22.061515604411152
19	23.00534149296497	25.041122421005156	30.266322948079626	21.687213137950245
20	23.729847802824626	24.727619232912087	30.02489454088807	21.51763842337522
21	22.7139471333693	25.56443839153851	30.51504726867533	21.206567206416864
22	21.47563164923017	25.919727265491655	30.094758438939838	22.509882646338337
23	22.261600502312582	26.189676061444857	31.02023399984082	20.528489436401745
24	22.34539296231771	26.242737249838605	28.683994092521026	22.727875695322656
25	22.18311416114683	26.336920859237512	29.716697471634372	21.763267507981286
26	21.940801400815374	27.11669924034065	29.63091521706448	21.3115841417795
27	21.19285973274848	27.134165214853596	29.219469918727945	22.453505133669978
28	21.399798367484106	26.665458050708807	29.57741185210078	22.357331729706306
29	21.845291261706627	26.71763488596266	28.919674204303263	22.51739964802745
30	21.64830159979483	26.721835563377166	30.56722410392918	21.062638732898822
31	22.12739991333339	26.649539694190683	30.49669694102249	20.726363451453434
32	21.77233212766522	26.431546645206367	29.376000424489508	22.42012080263891
33	21.47342076638043	26.452107855708938	30.01715645091398	22.05731492699665
34	20.922689848510306	26.884998717687946	30.444520105768635	21.747791328033113
35	20.6443396977281	28.65503152718944	29.4516126179506	21.249016157131866
36	22.634355350778673	26.644454663636285	29.177905321152842	21.543284664432203
37	21.530461543903712	27.216631145148877	29.197140001945577	22.05576730900183
38	21.35093785650486	25.90425108554348	30.662292066467984	22.08251899148368
39	20.880019809510333	25.873740902217072	31.318924272840633	21.92731501543196
40	19.933098684966883	26.293587555382615	31.006305437887455	22.767008321763047
41	21.1044244187589	25.272822943657864	31.112648902959926	22.51010373462331
42	20.05889791911706	26.997090478169746	30.504877207566526	22.43913439514667
43	20.358472545256774	26.657277784164773	29.871017094546193	23.11323257603226
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	59.0
2	90.0
3	194.0
4	298.0
5	298.0
6	499.5
7	701.0
8	794.0
9	887.0
10	1403.5
11	1920.0
12	1920.0
13	3612.0
14	5304.0
15	8326.5
16	11349.0
17	10198.0
18	9047.0
19	9047.0
20	9646.5
21	10246.0
22	8120.5
23	5995.0
24	6765.0
25	7535.0
26	7535.0
27	8315.0
28	9095.0
29	9906.0
30	10717.0
31	11958.0
32	13199.0
33	13199.0
34	14765.5
35	16332.0
36	18109.0
37	19886.0
38	21267.5
39	22649.0
40	22649.0
41	23574.5
42	24500.0
43	26280.0
44	28060.0
45	29211.0
46	30362.0
47	30362.0
48	38544.5
49	46727.0
50	44173.5
51	41620.0
52	38403.5
53	35187.0
54	35187.0
55	31122.0
56	27057.0
57	23505.0
58	19953.0
59	17602.5
60	15252.0
61	15252.0
62	13365.5
63	11479.0
64	10077.0
65	8675.0
66	7423.0
67	6171.0
68	6171.0
69	5299.0
70	4427.0
71	3663.5
72	2900.0
73	2417.0
74	1934.0
75	1934.0
76	1584.0
77	1234.0
78	963.0
79	692.0
80	546.0
81	400.0
82	400.0
83	299.5
84	199.0
85	150.5
86	102.0
87	77.5
88	53.0
89	53.0
90	43.0
91	33.0
92	20.5
93	8.0
94	6.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	452308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.45177776161699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.29917872743644	56.027398651465965
2	6.6344580406249145	8.4193631432501
3	1.7579118772900775	3.3462790128695072
4	0.8836787399216678	2.242839480727016
5	0.5086501450329093	1.6137377980521204
6	0.3504470365131392	1.3341892486829388
7	0.24671359703933882	1.0958091431076478
8	0.19610102812239907	0.9954367084197656
9	0.14138107295553126	0.8073792378785993
>10	0.8580576686125212	10.297565095806391
>50	0.07796918081194655	3.3954335578106654
>100	0.03950904674790082	4.609625509371295
>500	0.003496375818398302	1.5136911565328879
>1k	0.0020978254910389814	3.0846199385070014
>5k	3.4963758183983025E-4	1.2166323175181528
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5484	1.2124481547971737	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4726	1.0448632347869151	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3736	0.825985832662699	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2065	0.4565473084712187	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1162	0.25690458713973663	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1154	0.255135880859945	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1061	0.23457467035736712	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	996	0.22020393183405998	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	987	0.21821413726929437	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	931	0.20583319331075284	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	636	0.1406121492434359	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	601	0.13287405926934742	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	577	0.1275679404299725	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	546	0.12071420359577986	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	519	0.11474481990148305	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	517	0.11430264333153516	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	513	0.11341829019163932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.421765699479116E-4	0.0
10	4.421765699479116E-4	0.0	0.0	6.632648549218673E-4	0.0
11	4.421765699479116E-4	0.0	0.0	0.0013265297098437347	0.0
12	4.421765699479116E-4	0.0	0.0	0.002431971134713514	0.0
13	4.421765699479116E-4	0.0	0.0	0.0026530594196874694	0.0
14	4.421765699479116E-4	0.0	0.0	0.0026530594196874694	0.0
15	4.421765699479116E-4	0.0	0.0	0.0037585008445572485	0.0
16	4.421765699479116E-4	0.0	0.0	0.00420067741450516	0.0
17	4.421765699479116E-4	0.0	0.0	0.006632648549218674	0.0
18	4.421765699479116E-4	0.0	0.0	0.007295913404140542	0.0
19	6.632648549218673E-4	0.0	0.0	0.00795917825906241	0.0
20	6.632648549218673E-4	0.0	0.0	0.011275502533671745	0.0
21	6.632648549218673E-4	0.0	0.0	0.015697268233150864	0.0
22	6.632648549218673E-4	0.0	0.0	0.02365644649221327	0.0
23	6.632648549218673E-4	0.0	0.0	0.028520388761640297	0.0
24	6.632648549218673E-4	0.0	0.0	0.03537412559583293	0.0
25	6.632648549218673E-4	0.0	0.0	0.038911538155416224	0.0
26	6.632648549218673E-4	0.0	0.0	0.05107139382898379	0.0
27	6.632648549218673E-4	0.0	0.0	0.10965978934708208	0.0
28	6.632648549218673E-4	0.0	0.0	0.21202366529002362	0.0
29	6.632648549218673E-4	0.0	0.0	0.3577208450878605	0.0
30	6.632648549218673E-4	0.0	0.0	0.5595744492690822	0.0
31	6.632648549218673E-4	0.0	0.0	0.7775674982534025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAG	25	0.005492863	29.6	3
TAGTACT	85	6.91216E-11	26.117645	4
TATACCG	45	1.3214794E-4	24.666666	5
GGTATCA	1680	0.0	24.22619	1
AGGTTAC	40	0.0019293865	23.125	34
ATACCGC	40	0.0019293865	23.125	6
CGGTTGG	60	3.7198566E-5	21.583334	33
AAGACTA	45	0.0038222747	20.555555	5
ATTGTAC	45	0.0038222747	20.555555	3
CGGGAAC	55	5.1364687E-4	20.181818	6
TCGGGTA	65	6.890307E-5	19.923077	25
TTGGACC	270	0.0	19.87037	4
TGTGACG	75	9.24541E-6	19.733334	10
CGGGTAA	70	1.2171241E-4	18.5	26
AGTCAAT	60	9.225011E-4	18.5	27
TCGTCTT	50	0.007028447	18.5	14
GTACTAT	60	9.225011E-4	18.5	1
TATACTT	90	2.1463493E-6	18.5	5
ATTTCGG	70	1.2171241E-4	18.5	29
TGGACCC	305	0.0	18.19672	5
>>END_MODULE