FastQCFastQC Report
Wed 25 May 2016
SRR2088481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323010
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130760.988352317820727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110530.8354434206846509No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89740.6783017513095139No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.3718792752889245No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG26910.20339982313058858No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG26670.20158577788527676No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA24720.18684666026711816No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA23740.17943930884876153No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22490.16999115652942912No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG21080.15933364071322212No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.11065675996402144No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.101208607644689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37800.022.7089961
GTATTGG5150.021.5533981
TTGGACC6650.019.751884
ATTGGAC6150.018.3495943
TGGACCC6650.018.0827065
GGACCCT7200.017.4722236
TATTAGA3250.017.0769232
GTTAGGC803.3830493E-416.18753
ACCGTTA2200.015.9772738
GTACACG700.002592771315.8571424
CGGTATA700.002592771315.85714226
TAATACT4000.015.7254
AATACTG4150.015.6024095
TTAACGG2300.015.28260935
ATTAGAG3050.015.1639353
CAGATCG1252.9593775E-614.8000019
GTATCAA58250.014.70472052
CTAATAC3650.014.698633
CGGGTAA908.2751765E-414.38888926
CATACAC1803.3305696E-914.3888893
Produced by FastQC (version 0.11.5)