FastQCFastQC Report
Wed 25 May 2016
SRR2088480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309593
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT274041.1865294015006107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT232661.0073636350647062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT186490.8074582837755396No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102350.4431516721777387No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG57350.2483121485040871No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG55090.23852687464847702No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48690.210816364614891No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA48660.21068647159910858No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46840.20280629530830757No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG44320.19189528198258307No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30030.13002290879821685No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA28600.12383134171258746No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC26670.11547489103058417No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.11356979346577513No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26120.11309351907457287No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26060.112833733043008No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24560.1063390822538863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78450.022.2141481
ACGCCTA852.7250444E-517.4117646
GTATTGG10600.017.1037731
CTCTAAT5600.016.5178571
ATCGTAC700.002593556715.85714225
TCTAACG1201.937462E-615.4166662
CTAATAC6750.015.3481473
TCTAATA6350.015.1496072
TAATACT7100.015.1126764
ATACTGG6800.014.9632356
ACCTCTA3900.014.70512931
TATACTG2650.014.6603775
AGTTCCG2151.2732926E-1114.62790732
AATACTG7750.014.561295
GTATCAA121000.014.37190152
GTATTAG4950.014.2020211
TACTGGT6800.014.14705857
TACCCCG4350.014.0344835
ATCTCGT1203.303196E-513.8749996
TTGGACC15250.013.5868854
Produced by FastQC (version 0.11.5)