##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088480_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2309593
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.396016527587328	31.0	31.0	34.0	30.0	34.0
2	31.587582314286543	31.0	31.0	34.0	30.0	34.0
3	31.694503317251133	31.0	31.0	34.0	30.0	34.0
4	35.39229032994125	37.0	35.0	37.0	33.0	37.0
5	35.27009867106455	37.0	35.0	37.0	33.0	37.0
6	35.33131898130969	37.0	35.0	37.0	33.0	37.0
7	35.157924361565	37.0	35.0	37.0	32.0	37.0
8	35.209775488581755	37.0	35.0	37.0	32.0	37.0
9	36.746436709844545	39.0	35.0	39.0	32.0	39.0
10	36.59209782849186	39.0	35.0	39.0	32.0	39.0
11	36.630004940264364	39.0	35.0	39.0	32.0	39.0
12	36.477323493793065	38.0	35.0	39.0	32.0	39.0
13	36.497466436727166	38.0	35.0	39.0	32.0	39.0
14	37.44203199438169	39.0	36.0	41.0	32.0	41.0
15	37.471518574917745	39.0	36.0	41.0	32.0	41.0
16	37.33256119151729	39.0	36.0	41.0	32.0	41.0
17	37.40552642824948	39.0	36.0	41.0	32.0	41.0
18	37.28573692421132	39.0	36.0	41.0	32.0	41.0
19	37.36702267455781	39.0	36.0	41.0	32.0	41.0
20	37.26748349168014	39.0	36.0	41.0	31.0	41.0
21	37.11235789162853	39.0	36.0	40.0	31.0	41.0
22	37.01409599007271	39.0	36.0	40.0	31.0	41.0
23	36.811324765878666	39.0	35.0	40.0	30.0	41.0
24	37.0084776841634	39.0	36.0	40.0	31.0	41.0
25	36.963277945508146	39.0	36.0	41.0	31.0	41.0
26	36.787303217493296	39.0	36.0	40.0	30.0	41.0
27	36.726153915430125	39.0	35.0	40.0	30.0	41.0
28	36.52956083604341	39.0	35.0	40.0	30.0	41.0
29	36.356577544182024	39.0	35.0	40.0	30.0	41.0
30	36.18043612013026	38.0	35.0	40.0	30.0	41.0
31	35.888882586672196	38.0	35.0	40.0	29.0	41.0
32	35.75174890121333	38.0	34.0	40.0	28.0	41.0
33	35.54040170714061	38.0	34.0	40.0	27.0	41.0
34	35.4160217839247	38.0	34.0	40.0	27.0	41.0
35	35.17559803826908	38.0	34.0	40.0	26.0	41.0
36	35.0235573973423	38.0	33.0	40.0	25.0	41.0
37	34.85732204765082	38.0	33.0	40.0	25.0	41.0
38	34.765931053653176	38.0	33.0	40.0	24.0	41.0
39	34.65697116331752	38.0	33.0	40.0	24.0	41.0
40	34.5306748851421	38.0	33.0	40.0	23.0	41.0
41	34.30274987844179	38.0	33.0	40.0	22.0	41.0
42	34.06598911583123	38.0	33.0	40.0	21.0	41.0
43	33.727601356602655	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	3.0
14	13.0
15	18.0
16	31.0
17	98.0
18	225.0
19	550.0
20	1140.0
21	2248.0
22	4153.0
23	7054.0
24	11255.0
25	16869.0
26	24226.0
27	33159.0
28	44654.0
29	57854.0
30	72008.0
31	88208.0
32	107445.0
33	130303.0
34	157264.0
35	190398.0
36	239321.0
37	317050.0
38	404244.0
39	399799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52441577368827	21.336183474750747	13.693668105159654	23.445732646401336
2	19.382592517382932	23.68018953988863	34.48196283934009	22.455255103388346
3	19.63895803286553	24.88273908000241	32.54993412259216	22.9283687645399
4	13.958346773652327	16.534558253337277	36.26755882962929	33.2395361433811
5	12.481116802830629	38.826624431230954	34.89562879693522	13.796629969003197
6	32.2802329241559	37.595022153253844	13.495191577044094	16.629553345546164
7	26.112739344118207	32.917098380537176	21.50283621399961	19.46732606134501
8	27.24055710248516	34.327390150559	18.82136809385896	19.61068465309689
9	24.79856840577539	14.235798255363608	21.524311859275638	39.44132147958536
10	16.764642081959895	27.530391718367696	33.119082020078864	22.585884179593545
11	33.54586717226801	22.621994437981062	23.089609294797828	20.742529094953095
12	21.216725197902832	27.39430713549963	30.57789835698324	20.81106930961429
13	31.37868879928195	21.128701030874268	25.879884464492232	21.612725705351547
14	20.735817955804333	23.084413574166533	27.434400779704475	28.74536769032466
15	23.01738877802279	29.544296332730486	25.33814399333562	22.1001708959111
16	22.194949499760348	28.28450727032858	26.258306117138385	23.26223711277268
17	22.009722059254596	26.90950310292766	26.789698444704324	24.29107639311342
18	22.96906857615173	25.754277918230617	29.103699223196465	22.17295428242119
19	23.055187645615483	25.002890119601158	30.13344775464768	21.80847448013568
20	23.748729754549828	24.751936813109495	30.000870283205742	21.498463149134935
21	22.693911871052606	25.461369167641223	30.41791345921121	21.426805502094957
22	21.593631432031533	25.98492461658829	29.961642592439446	22.45980135894073
23	22.403904064482358	26.14395696557792	30.87760484206525	20.57453412787448
24	22.48707889225504	26.25644431724551	28.430550317739968	22.825926472759487
25	22.28535503874492	26.275148911518176	29.64258204800586	21.79691400173104
26	22.028166867495703	27.04099813257141	29.597465873857427	21.33336912607546
27	21.28288404060802	27.046237150874635	29.105257939385858	22.565620869131486
28	21.532971393661136	26.67734098605252	29.40085114563475	22.388836474651594
29	22.076183985663274	26.653050992101207	28.871623701665182	22.399141320570333
30	21.871299402102448	26.749041930764424	30.415965063974475	20.96369360315865
31	22.299340186777496	26.544547026250946	30.285682369144695	20.870430417826864
32	21.843372403709225	26.348711656122966	29.34252918154844	22.465386758619378
33	21.48841808924776	26.46371027276234	30.00368463188103	22.044187006108867
34	20.97711588145617	26.830181767956518	30.440125164910008	21.752577185677303
35	20.684466916898344	28.594258815297763	29.368464487033	21.352809780770897
36	22.719457497489817	26.508826446910778	29.15124006697284	21.620475988626566
37	21.577221614371016	27.06481185213152	29.282561905928876	22.075404627568577
38	21.376320416627518	25.76640126637031	30.653799175872116	22.20347914113006
39	20.870949989889994	25.81337924041162	31.2358497795932	22.079820990105183
40	20.094319648526817	26.353301209347276	30.88319024174389	22.669188900382014
41	21.137403862931695	25.140879800034032	31.066036310293633	22.655680026740644
42	20.006771755889456	26.95855936522149	30.557375260489618	22.477293618399433
43	20.305568989860983	26.486614741211977	29.994722013792043	23.213094255134994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	297.0
2	421.0
3	970.5
4	1520.0
5	1520.0
6	2484.5
7	3449.0
8	3970.0
9	4491.0
10	7070.0
11	9649.0
12	9649.0
13	18088.5
14	26528.0
15	41526.0
16	56524.0
17	51284.5
18	46045.0
19	46045.0
20	49203.0
21	52361.0
22	41584.0
23	30807.0
24	34504.0
25	38201.0
26	38201.0
27	42101.5
28	46002.0
29	50482.5
30	54963.0
31	61096.0
32	67229.0
33	67229.0
34	75634.5
35	84040.0
36	92903.0
37	101766.0
38	109188.0
39	116610.0
40	116610.0
41	120884.0
42	125158.0
43	134242.0
44	143326.0
45	149173.5
46	155021.0
47	155021.0
48	194269.0
49	233517.0
50	222011.0
51	210505.0
52	193457.5
53	176410.0
54	176410.0
55	158176.0
56	139942.0
57	121020.5
58	102099.0
59	90867.5
60	79636.0
61	79636.0
62	70437.5
63	61239.0
64	53243.5
65	45248.0
66	38825.5
67	32403.0
68	32403.0
69	27874.0
70	23345.0
71	19684.0
72	16023.0
73	13230.0
74	10437.0
75	10437.0
76	8430.5
77	6424.0
78	5082.5
79	3741.0
80	2916.5
81	2092.0
82	2092.0
83	1590.5
84	1089.0
85	848.5
86	608.0
87	449.5
88	291.0
89	291.0
90	218.0
91	145.0
92	103.5
93	62.0
94	50.5
95	39.0
96	39.0
97	24.5
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2309593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.28494576398399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.66745985548305	39.75691383895838
2	11.1133493706233	10.9544164197475
3	3.4029956537634845	5.031493686924198
4	1.464804418591518	2.8877122530050827
5	0.7639814012439204	1.8826390962499557
6	0.4465759968433153	1.320568427035193
7	0.32070209912365333	1.106404989319355
8	0.23401213053995956	0.9226620129420998
9	0.16569746514510053	0.7349751524615317
>10	1.18167779566542	11.37245570271934
>50	0.13235408944266405	4.474007144635536
>100	0.09360147805322815	8.975551279163483
>500	0.00811392127102322	2.801997594157119
>1k	0.004145155431935772	3.831507747541405
>5k	1.7638959284833075E-4	0.4887402222779366
>10k+	3.527791856966615E-4	3.4579544328618788
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27404	1.1865294015006107	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23266	1.0073636350647062	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18649	0.8074582837755396	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10235	0.4431516721777387	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5735	0.2483121485040871	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5509	0.23852687464847702	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4869	0.210816364614891	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4866	0.21068647159910858	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4684	0.20280629530830757	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4432	0.19189528198258307	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3003	0.13002290879821685	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2860	0.12383134171258746	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2667	0.11547489103058417	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2623	0.11356979346577513	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2612	0.11309351907457287	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2606	0.112833733043008	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2456	0.1063390822538863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.329767192747813E-5	0.0
3	0.0	0.0	0.0	4.329767192747813E-5	0.0
4	0.0	0.0	0.0	4.329767192747813E-5	0.0
5	4.329767192747813E-5	0.0	0.0	4.329767192747813E-5	0.0
6	8.659534385495626E-5	0.0	0.0	4.329767192747813E-5	0.0
7	8.659534385495626E-5	0.0	0.0	4.329767192747813E-5	0.0
8	8.659534385495626E-5	0.0	0.0	8.659534385495626E-5	0.0
9	8.659534385495626E-5	0.0	0.0	1.7319068770991252E-4	0.0
10	2.1648835963739065E-4	0.0	0.0	3.030837034923469E-4	0.0
11	2.1648835963739065E-4	0.0	0.0	5.628697350572157E-4	0.0
12	2.1648835963739065E-4	0.0	0.0	6.927627508396501E-4	0.0
13	2.1648835963739065E-4	0.0	0.0	7.360604227671282E-4	0.0
14	2.597860315648688E-4	0.0	0.0	8.226557666220845E-4	0.0
15	3.030837034923469E-4	0.0	0.0	9.525487824045189E-4	0.0
16	3.4638137541982504E-4	0.0	0.0	0.0015587161893892128	0.0
17	3.896790473473032E-4	0.0	0.0	0.002251478940228863	0.0
18	3.896790473473032E-4	0.0	0.0	0.002641157987576166	0.0
19	4.7627439120225944E-4	0.0	0.0	0.0037235997857631192	0.0
20	4.7627439120225944E-4	0.0	0.0	0.005282315975152332	0.0
21	4.7627439120225944E-4	0.0	0.0	0.009135808776697885	0.0
22	4.7627439120225944E-4	0.0	4.329767192747813E-5	0.014331529407995262	0.0
23	4.7627439120225944E-4	0.0	4.329767192747813E-5	0.018011831521830904	0.0
24	4.7627439120225944E-4	0.0	4.329767192747813E-5	0.023294147496983234	0.0
25	4.7627439120225944E-4	0.0	4.329767192747813E-5	0.027537319345876093	0.0
26	5.195720631297376E-4	0.0	4.329767192747813E-5	0.03719270018570371	0.0
27	5.195720631297376E-4	0.0	4.329767192747813E-5	0.10144644532608126	0.0
28	5.195720631297376E-4	0.0	4.329767192747813E-5	0.21518942947956632	0.0
29	5.195720631297376E-4	0.0	4.329767192747813E-5	0.3637004441908163	0.0
30	5.195720631297376E-4	0.0	4.329767192747813E-5	0.5659871674359941	0.0
31	5.195720631297376E-4	0.0	4.329767192747813E-5	0.795897805370903	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7845	0.0	22.214148	1
ACGCCTA	85	2.7250444E-5	17.411764	6
GTATTGG	1060	0.0	17.103773	1
CTCTAAT	560	0.0	16.517857	1
ATCGTAC	70	0.0025935567	15.857142	25
TCTAACG	120	1.937462E-6	15.416666	2
CTAATAC	675	0.0	15.348147	3
TCTAATA	635	0.0	15.149607	2
TAATACT	710	0.0	15.112676	4
ATACTGG	680	0.0	14.963235	6
ACCTCTA	390	0.0	14.705129	31
TATACTG	265	0.0	14.660377	5
AGTTCCG	215	1.2732926E-11	14.627907	32
AATACTG	775	0.0	14.56129	5
GTATCAA	12100	0.0	14.3719015	2
GTATTAG	495	0.0	14.202021	1
TACTGGT	680	0.0	14.1470585	7
TACCCCG	435	0.0	14.034483	5
ATCTCGT	120	3.303196E-5	13.874999	6
TTGGACC	1525	0.0	13.586885	4
>>END_MODULE