FastQCFastQC Report
Wed 25 May 2016
SRR2088479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500654
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT286911.1473398558936982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT253481.013654827897022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198500.7937923439228297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109270.4369656897755547No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG66450.26573048490514883No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59810.23917743118400228No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA54630.21846285011840905No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA53390.2135041473150624No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA53130.21246441930790907No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG45800.18315208741393252No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31800.12716673318259944No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA29780.1190888463577928No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29700.11876893004789948No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.11780918111821947No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28100.11237060385003284No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28010.11201069800140283No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.10265314593702288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80250.023.7445471
TATACCG1151.3460522E-1020.9130445
TAATACT7250.017.0965524
CTAATAC6500.016.7923073
ACCGTTA4000.016.18758
CTCTAAT5750.016.0869561
ATACTGG6500.015.9384616
AATACTG8050.015.627335
TTACTCG855.367283E-415.23529419
GTATCAA125700.015.1738262
TCTAATA6300.014.976192
CGAATTA1252.9619296E-614.79999915
TCGCGAC908.2788104E-414.38888919
GTATTGG9400.014.3670221
CCGTTTA4650.014.3225827
GATGCTA6050.014.06611514
GTATTAG6000.013.8751
TTATGCG3900.013.7564114
TCTATAC1753.57104E-813.7428583
TAGGACC8650.013.68786054
Produced by FastQC (version 0.11.5)