FastQCFastQC Report
Wed 25 May 2016
SRR2088478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187710
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT277891.2702323434093186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT241651.1045796746369492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT191010.8731047533722477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104570.47798839882799826No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG59410.2715625014284343No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG57930.2647974365889446No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA53670.24532502022662966No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA52640.24061690077752537No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA51080.2334861567575227No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG47390.21661920455636258No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.14270630019518124No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA29520.13493561760928094No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28100.12844481215517597No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27850.12730206471607297No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27420.12533653912081583No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26140.11948567223260852No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.11427474391029889No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA23630.10801248794401451No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC22160.10129313300208895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79600.022.8925881
GTATTGG10250.019.1317061
TTGGACC14150.017.5194364
ATTGGAC11350.016.9515443
CTCTAAT4650.016.7096771
TGGACCC14050.016.5907485
TATACTG2600.016.3653835
TCTAATA5300.015.7075472
GGACCCT14750.015.6779666
CTAATAC5550.015.6666673
TATTGGA12400.015.3669352
GTATTAG3950.014.9873421
GTATCAA122850.014.8030122
CGTTTAC3000.014.828
TAATACT5550.014.6666674
CGAACTA2151.2732926E-1114.62790724
AGTTCCG2301.8189894E-1214.47826132
TGCTAGA3850.014.41558416
TTTACCG908.278228E-414.38888830
CCGTTTA3100.014.3225827
Produced by FastQC (version 0.11.5)