##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088478_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2187710
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.401885990373497	31.0	31.0	34.0	30.0	34.0
2	31.59036572489041	31.0	31.0	34.0	30.0	34.0
3	31.697210324951662	31.0	31.0	34.0	30.0	34.0
4	35.39071723400268	37.0	35.0	37.0	33.0	37.0
5	35.27601875934196	37.0	35.0	37.0	33.0	37.0
6	35.33625389105503	37.0	35.0	37.0	33.0	37.0
7	35.159848425979675	37.0	35.0	37.0	32.0	37.0
8	35.21855684711411	37.0	35.0	37.0	32.0	37.0
9	36.74848997353397	39.0	35.0	39.0	32.0	39.0
10	36.606776492313884	39.0	35.0	39.0	32.0	39.0
11	36.64111559575995	39.0	35.0	39.0	32.0	39.0
12	36.48566446192594	38.0	35.0	39.0	32.0	39.0
13	36.51129400149014	38.0	35.0	39.0	32.0	39.0
14	37.45167595339419	39.0	36.0	41.0	32.0	41.0
15	37.48414826462374	39.0	36.0	41.0	32.0	41.0
16	37.34607603384361	39.0	36.0	41.0	32.0	41.0
17	37.42135932093376	39.0	36.0	41.0	32.0	41.0
18	37.29726746232362	39.0	36.0	41.0	32.0	41.0
19	37.38195510373861	39.0	36.0	41.0	32.0	41.0
20	37.28355449305438	39.0	36.0	41.0	31.0	41.0
21	37.12687970526258	39.0	36.0	40.0	31.0	41.0
22	37.0299605523584	39.0	36.0	40.0	31.0	41.0
23	36.825928025195296	39.0	35.0	40.0	30.0	41.0
24	37.020913649432515	39.0	36.0	40.0	31.0	41.0
25	36.98895374615466	39.0	36.0	41.0	31.0	41.0
26	36.802162992352734	39.0	36.0	40.0	30.0	41.0
27	36.73685726170287	39.0	35.0	40.0	30.0	41.0
28	36.53342353419786	39.0	35.0	40.0	30.0	41.0
29	36.358167673046246	39.0	35.0	40.0	30.0	41.0
30	36.17652202531414	38.0	35.0	40.0	30.0	41.0
31	35.87591042688474	38.0	35.0	40.0	29.0	41.0
32	35.724946176595616	38.0	34.0	40.0	28.0	41.0
33	35.503921909211	38.0	34.0	40.0	27.0	41.0
34	35.37213981743467	38.0	34.0	40.0	27.0	41.0
35	35.130752247784216	38.0	34.0	40.0	25.0	41.0
36	34.9701294961398	38.0	33.0	40.0	25.0	41.0
37	34.80162772945226	38.0	33.0	40.0	24.0	41.0
38	34.709425837976696	38.0	33.0	40.0	24.0	41.0
39	34.591775418131284	38.0	33.0	40.0	24.0	41.0
40	34.45074027179105	38.0	33.0	40.0	23.0	41.0
41	34.21767556028907	38.0	33.0	40.0	22.0	41.0
42	33.977997540807515	38.0	33.0	40.0	21.0	41.0
43	33.62319868721174	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	7.0
15	22.0
16	30.0
17	99.0
18	182.0
19	479.0
20	1050.0
21	2098.0
22	3984.0
23	6806.0
24	10741.0
25	16065.0
26	23206.0
27	31884.0
28	42885.0
29	54919.0
30	68313.0
31	83774.0
32	101674.0
33	123480.0
34	149326.0
35	180281.0
36	227489.0
37	301295.0
38	379729.0
39	377885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32394147304716	21.43277673914733	14.069872149416513	23.173409638389
2	19.35078232489681	23.74025807808165	34.553482865644895	22.355476731376644
3	19.781369559950814	24.77618148657729	32.67055505528612	22.771893898185773
4	14.13130625174269	16.561792925022058	35.80986511009229	33.497035713142964
5	12.5052223557967	38.85039607626239	34.731980015632786	13.91240155230812
6	32.50023997696221	37.229843077921664	13.385914952164594	16.884001992951532
7	25.94644628401388	33.115312358585	21.309999954290102	19.628241403111016
8	27.24213904036641	34.503796206992696	18.755456619021718	19.49860813361917
9	24.616928203463896	14.44775587257909	21.608485585383804	39.32683033857321
10	16.743946866815072	27.72853806034621	32.965749573755204	22.561765499083517
11	33.27611977821558	22.58489470725096	23.214137157118632	20.92484835741483
12	21.08465015929899	27.501542709042788	30.579372951625217	20.834434180033
13	31.759693926525912	21.050596285613725	25.96623866965914	21.223471118201225
14	20.502168934639418	23.267023508600317	27.517358333599972	28.713449223160293
15	22.783915601245138	29.658272805810643	25.41310319923573	22.14470839370849
16	21.77656087872707	28.423511342911077	26.444912716950604	23.355015061411248
17	21.88873296734942	26.810134798487915	26.89460668918641	24.406525544976255
18	23.076367525860377	25.57317926050528	29.29597615771743	22.05447705591692
19	22.99587239624996	24.936714646822477	30.385243016670398	21.682169940257165
20	23.854852791274894	24.562761974850414	30.17388045033391	21.40850478354078
21	22.628090560449053	25.427821786251375	30.760795535057206	21.183292118242363
22	21.408779042926167	25.789524205676255	30.296337265908186	22.505359485489393
23	22.256971902125965	26.010211591115823	31.202033176243653	20.530783330514556
24	22.23393411375365	26.0525389562602	28.954980321889103	22.758546608097053
25	22.26218283044828	26.185874727454735	29.904831993271504	21.647110448825487
26	22.0458378852773	26.855113337691012	29.87585191821585	21.22319685881584
27	21.003789350508065	26.9708050884258	29.502219215526736	22.5231863455394
28	21.489457012126838	26.56782663150052	29.66069543038154	22.282020925991105
29	21.762710779765143	26.482577672543435	29.13951117835545	22.615200369335973
30	21.73514771153398	26.61920455636259	30.701875477097058	20.943772255006376
31	22.315206311622656	26.38690685694173	30.514373477289038	20.783513354146574
32	21.95240685465624	26.287807799022723	29.470587966412364	22.28919737990867
33	21.537955213442366	26.2595590823281	30.152853897454417	22.04963180677512
34	20.75796152140823	26.835458081738437	30.69049371260359	21.716086684249742
35	20.55510099601867	28.592089445127556	29.65703863857641	21.19577092027737
36	22.56601651955698	26.351298846739287	29.587605304176513	21.495079329527222
37	21.58393937039187	27.081285910838275	29.487454918613526	21.847319800156328
38	21.47185870156465	25.570436666651432	30.864191323347246	22.093513308436677
39	20.841016405282236	25.49117570427525	31.60455453419329	22.06325335624923
40	19.97869918773512	26.04723660814276	31.170539056821973	22.803525147300142
41	21.217803090903274	25.08673453062792	31.25761641168162	22.437845966787187
42	19.912739805550096	26.949184306877967	30.71376005046373	22.424315837108207
43	20.326048699324865	26.73123951529225	29.9849614437014	22.957750341681486
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	278.5
2	411.0
3	1003.5
4	1596.0
5	1596.0
6	2543.0
7	3490.0
8	3905.0
9	4320.0
10	6855.5
11	9391.0
12	9391.0
13	17997.5
14	26604.0
15	41661.0
16	56718.0
17	51226.5
18	45735.0
19	45735.0
20	49029.0
21	52323.0
22	41449.0
23	30575.0
24	34117.5
25	37660.0
26	37660.0
27	41379.5
28	45099.0
29	49057.0
30	53015.0
31	58292.0
32	63569.0
33	63569.0
34	71005.5
35	78442.0
36	85706.0
37	92970.0
38	98862.5
39	104755.0
40	104755.0
41	108673.0
42	112591.0
43	120149.0
44	127707.0
45	136423.5
46	145140.0
47	145140.0
48	187328.0
49	229516.0
50	215316.5
51	201117.0
52	185193.5
53	169270.0
54	169270.0
55	151044.5
56	132819.0
57	114368.0
58	95917.0
59	85313.0
60	74709.0
61	74709.0
62	65819.5
63	56930.0
64	49545.0
65	42160.0
66	36653.5
67	31147.0
68	31147.0
69	26827.0
70	22507.0
71	19133.5
72	15760.0
73	12877.5
74	9995.0
75	9995.0
76	8060.5
77	6126.0
78	4782.0
79	3438.0
80	2677.5
81	1917.0
82	1917.0
83	1483.0
84	1049.0
85	783.5
86	518.0
87	406.5
88	295.0
89	295.0
90	225.0
91	155.0
92	113.5
93	72.0
94	51.0
95	30.0
96	30.0
97	16.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2187710.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.86578703356584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87342090607473	41.13690204484328
2	10.932630463213485	11.121937057169825
3	3.3651957885197343	5.135199969152924
4	1.4102494317675063	2.8693378904197284
5	0.7912324410038948	2.0123330419076275
6	0.47395723364225445	1.446492462567895
7	0.33319795573432387	1.1863863379481132
8	0.2430671434023248	0.9891041240927888
9	0.18775107442795258	0.8595095550457864
>10	1.1865382461980467	11.573421112250504
>50	0.11506500200427988	4.042373021071443
>100	0.07832310121800004	7.5863673424886775
>500	0.004685606396728491	1.652684154784526
>1k	0.0038746360588331735	3.3927280078057613
>5k	4.505390766085086E-4	1.2591988403237915
>10k+	3.6043126128680684E-4	3.7360250381273565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27789	1.2702323434093186	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24165	1.1045796746369492	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19101	0.8731047533722477	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10457	0.47798839882799826	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5941	0.2715625014284343	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5793	0.2647974365889446	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5367	0.24532502022662966	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5264	0.24061690077752537	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5108	0.2334861567575227	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4739	0.21661920455636258	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3122	0.14270630019518124	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2952	0.13493561760928094	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2810	0.12844481215517597	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2785	0.12730206471607297	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2742	0.12533653912081583	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2614	0.11948567223260852	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2500	0.11427474391029889	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2363	0.10801248794401451	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2216	0.10129313300208895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.570989756411956E-5	0.0	0.0	0.0	0.0
6	4.570989756411956E-5	0.0	0.0	0.0	0.0
7	4.570989756411956E-5	0.0	0.0	0.0	0.0
8	4.570989756411956E-5	0.0	0.0	0.0	0.0
9	4.570989756411956E-5	0.0	0.0	2.2854948782059778E-4	0.0
10	9.141979512823912E-5	0.0	0.0	4.11389078077076E-4	0.0
11	9.141979512823912E-5	0.0	0.0	7.313583610259129E-4	0.0
12	9.141979512823912E-5	0.0	0.0	9.599078488465107E-4	0.0
13	9.141979512823912E-5	0.0	0.0	9.599078488465107E-4	0.0
14	9.141979512823912E-5	0.0	0.0	0.0012798771317953477	0.0
15	9.141979512823912E-5	0.0	0.0	0.0015998464147441846	0.0
16	1.3712969269235868E-4	0.0	0.0	0.002605464161154815	0.0
17	1.3712969269235868E-4	0.0	0.0	0.003748211600257804	0.0
18	1.3712969269235868E-4	0.0	0.0	0.004251020473463119	0.0
19	1.3712969269235868E-4	0.0	0.0	0.0051652184247455105	0.0
20	1.3712969269235868E-4	0.0	0.0	0.008136361766413282	0.0
21	1.3712969269235868E-4	0.0	0.0	0.013758679166799986	0.0
22	1.3712969269235868E-4	0.0	0.0	0.0226721091918033	0.0
23	1.3712969269235868E-4	0.0	0.0	0.02806587710436941	0.0
24	1.3712969269235868E-4	0.0	0.0	0.03629365866591093	0.0
25	1.3712969269235868E-4	0.0	0.0	0.042053105758989996	0.0
26	1.3712969269235868E-4	0.0	0.0	0.05192644363283982	0.0
27	1.3712969269235868E-4	0.0	0.0	0.1152346517591454	0.0
28	1.3712969269235868E-4	0.0	0.0	0.23970270282624295	0.0
29	1.3712969269235868E-4	0.0	0.0	0.39292227946117175	0.0
30	1.8283959025647823E-4	0.0	0.0	0.6127411768470227	0.0
31	1.8283959025647823E-4	0.0	0.0	0.865928299454681	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7960	0.0	22.892588	1
GTATTGG	1025	0.0	19.131706	1
TTGGACC	1415	0.0	17.519436	4
ATTGGAC	1135	0.0	16.951544	3
CTCTAAT	465	0.0	16.709677	1
TGGACCC	1405	0.0	16.590748	5
TATACTG	260	0.0	16.365383	5
TCTAATA	530	0.0	15.707547	2
GGACCCT	1475	0.0	15.677966	6
CTAATAC	555	0.0	15.666667	3
TATTGGA	1240	0.0	15.366935	2
GTATTAG	395	0.0	14.987342	1
GTATCAA	12285	0.0	14.803012	2
CGTTTAC	300	0.0	14.8	28
TAATACT	555	0.0	14.666667	4
CGAACTA	215	1.2732926E-11	14.627907	24
AGTTCCG	230	1.8189894E-12	14.478261	32
TGCTAGA	385	0.0	14.415584	16
TTTACCG	90	8.278228E-4	14.388888	30
CCGTTTA	310	0.0	14.32258	27
>>END_MODULE